Asian Journal of Research in Chemistry

Amit Rathi ¹, Dinesh Dhamecha ¹, Saifee Maria ¹, Mohd Hassan G. Dehghan ¹

Affiliations
1 Y.B.Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Maulana Azad Education Trust, Rauza Bagh, Aurangabad (MS) 431001, IN

Abstract

The present research work discusses the development of a UV estimation method for Idebenone. Simple, accurate, cost efficient and reproducible spectrophotometric method has been developed for the estimation of Idebenone in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement at maximum wavelength (λmax) 282nm. The percentage recovery of Idebenone ranged from 99.68 to 99.82% in pharmaceutical dosage form. Beers law was obeyed in the concentration range of 4-20μg/ml having line equation y = 0.0485x - 0.0239 with correlation coefficient of 0.9978. Results of the analysis were validated statistically and by recovery study.

Keywords

UV Spectrophotometry, Idebenone.

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Santosh Shelke ¹, Santosh Dongre ¹, Amit Rathi ², Dinesh Dhamecha ², Saifee Maria ², Mohd Hassan G. Dehghan ²

Affiliations
1 Yash Institute of Pharmacy, South City, Bajaj Nagar, Post Box no. 968, Waluj, Aurangabad-431134, IN
2 Y.B.Chavan College of Pharmacy, Maulana Azad Education Trust, Dr. Rafiq Zakaria Marg, Aurangabad-431001, IN

Abstract

A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of cefuroxime axetil in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement of absorption at maximum wavelength 281nm.The percentage recovery of cefuroxime axetil ranged from (99.97 ± 0.3969) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 4-28μg/ml having line equation y = 0.0346x + 0.0566 with correlation coefficient of 0.9999. Results of the analysis were validated statistically and by recovery study.

Keywords

UV Spectrophotometry, Cefuroxime Axetil.

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