Asian Journal of Research in Chemistry

Ravi Kumar vejendla ¹, G. Hemanth Kumar ¹, G. Raveendra Babu ², J. Srinivasarao ³

Affiliations
1 Sri Indu Institute of Pharmacy, Sheriguda (Village), Ibrahimpatan-501 510, RR Dist., Andhra Pradesh, IN
2 Dr.Reddy’s Laboratories Ltd, Bachupally, Hyderabad, Andhra Pradesh, IN
3 United States Pharmacopeia-India Private Limited, ICICI Knowledge Park, Turkapally, Shameerpet, Hyderabad, IN

Abstract

A novel stability indicating liquid chromatographic assay method was developed and validated as per ICH guidelines for the quantitative estimation of Donepezil in tablet formulation. An isocratic reverse phase LC-method was developed using Zorbax SB C18, 150 x 4.6mm, 5μm column and a mobile phase comprising of a mixture of water: acetonitrile (68:32), pH adjusted to 4.5 with trifluoro acetic acid. The detector set at 229nm with flow rate of 1.0mL min^-1. The method is linear between 5 μg mL^-1 to 25 μg mL^-1 and the limit of detection (LOD) is 0.5 μg mL^-1. The Accuracy of the method was found to be in the range of 99.70% to 100.26%. The mean Inter and Intraday assay Relative Standard deviation (%RSD) were less than 0.69%. The Proposed method was found to be Linear, precise and accurate for the quantitative estimation of Donepezil in tablet formulations and can be used for commercial purposes.

Keywords

Donepezil, Liquid Chromatography, Stress Degradation and Method Validation.

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M. Ramayyappa ¹, G. Raveendra Babu ¹, H.S.P. Syloosha ¹, V. Alekya Satyasri ¹, G. premi ¹, Ch. Prudhvi ¹

Affiliations
1 Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, IN

Abstract

Oils and fats in the diet serve three functions in the human body: as an energy source, a structural component, and as important biological regulators. Lipid peroxidation may be accelerated by repeatedly heating soy oil. Unsaturated fatty acids that have been oxidised may play a role in the aetiology of a variety of diseases. The purpose of this study was to see if repeatedly heating edible oil had any negative consequences on illness development. In this investigation, four different edible oils were heated to their regular boiling point and then heated for another 3 hours at a 1hour interval. The heated samples were subjected to qualitative examination. After analysing the results, it was discovered that saponification values declined as continuous heating, i.e. 3 hours, was applied to all different oils, resulting in low saponification values when compared to 2 hours, 1 hour, and normal boiling point. Lower the saponification value, the more free fatty acids and triglycerides are produced, which may contribute to cardio vascular illness. The acid values grew as the heating progressed, according to the findings. A high acid number suggests that there is a lot of acid in the system. The values of iodine fell as the heating progressed, according to the results. The presence of saturated fatty acids is indicated by a low iodine value. Saturated fatty acids were detrimental to one's health. As the heating progressed, the peroxide levels grew. Deterioration is indicated by an increase in peroxide value. When compared to other oils, palm oil showed a high saponification value, acid value, peroxide value, and low iodine value. Total cholesterol, tri-glycerides, and glucose levels were higher in 3 hours heated oils than in 2 hours, 1 hour, and at normal boiling point in biological tests .

Keywords

Edible oil, Heating, Fatty acids, Deterioration, Peroxide values.

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Nalla Priyanka ¹, M. Sowjanya ², Miriyala Mrunalini ³, Ayinam Vasavi ⁴, G. Raveendra Babu ⁵

Affiliations
1 Department of Pharmaceutical Chemistry, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh., IN
2 Department of Chemistry, Vijaya Teja Degree College, Addanki-523201, Andhra Pradesh ., IN
3 Department of Pharmaceutics, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh ., IN
4 Department of Pharmaceutics, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh., IN
5 Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh., IN

Abstract

The study's goal is to synthesis novel benzodiazepine analogues, some of which are chalcones. The elemental analysis, infrared, and 1H nuclear magnetic resonance examinations examined the structures of the newly produced substances. To test for anti-inflammatory and anti-oxidant properties, all substances bearing a descriptor were evaluated. Here, 1,5-(disubstituted phenyl)-2,4-pentadien-1-one derivatives are created from scratch using a new process. By condensing cinnamaldehyde with different aromatic ketones in the presence of 10% NaOH as a base, 1,5-(disubstituted phenyl)-2,4-pentadien-1-one was produced. 2-(substituted phenyl)-3- styryl-2,3-dihydro-1H-benzodiazepine derivatives were synthesised from 1,5-(disubstituted phenyl)-2,4- pentadien-1-one on cyclization with o-phenylene diamine in the presence of NaOH as base. Carrageenan and formalin-induced rat paw edoema technique was used to evaluate the antioxidant and anti-inflammatory activities of the final synthesised benzodiazepine derivatives. Compared to the usual ascorbic acid and diclofenac sodium, the compounds displayed significant anti-inflammatory effect. This means that nitro, chloro, fluoro, and bromo included electron withdrawing groups, which caused an increase in anti-inflammatory action. Most of the produced drugs had strong anti-inflammatory efficacy .

Keywords

Chalcones, 1,5-benzodiazepines, Anti-inflammatory activity, Anti-oxidant activity.

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