Asian Journal of Research in Chemistry

M. Seethamma ¹, B. Vijayakumar ¹, G. Sai Prasad ¹, G. Venkateshwarlu ¹

Affiliations
1 Department of Chemistry, Nizam College (O.U), Hyderabad, 500001, IN

Abstract

Three simple and sensitive extractive spectrophotometric methods have been described for the assay of Esmolol hydrochloride either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with bromothymol blue (BTB), bromophenol blue (BPB) and bromocresol green (BCG) in acidic medium. The extracted complexes showed absorbances maxima at 415,412 and 412nm with BTB, BPB and BCG respectively. Beer's law is obeyed in the concentration ranges 2.5- 25μg/ml for all three dyes. The effect of concentration of dye and pH have been studied and optimized. The limits of detection and quantification have been determined for three methods. All the three methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.

Keywords

Esmolol Hydrochloride, Bromothymol Blue, Bromophenol Blue, Bromocresol Green, Spectrophotometry.

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M. Chennaiah ¹, T. Veeraiah ², G. Venkateshwarlu ¹

Affiliations
1 Department of Chemistry, Nizam College, Hyderabad, 500001, IN
2 Department of Chemistry, SAP College Vikarabad, Ranga Reddy, Andhra Pradesh, 501101, IN

Abstract

Three simple and sensitive extractive spectrophotometric methods have been described for the assay of Ambroxol hydrochloride either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with bromothymol blue (BTB), bromophenol blue (BPB) and bromocresol purple (BCP) in acidic medium. The extracted complexes showed absorbance maxima at 414, 412 and 407nm with use of the cited reagents, respectively. Beer's law is obeyed in the concentration ranges 2.5-25, 4.0-30 and 4.0-40μg/ml with BTB, BPB and BCP respectively. The effect of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined for three methods. All the three methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.

Keywords

Ambroxol Hydrochloride, Bromothymol Blue, Bromophenol Blue, Bromocresol Purple, Spectrophotometry.

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K. Susmitha ¹, K. Radha ¹, G. Venkateshwarlu ²

Affiliations
1 Department of Chemistry, St. Anns College for Women, Hyderabad -500028, A.P., IN
2 Department of Chemistry, Nizam College, Hyderabad -500001, A.P., IN

Abstract

Four simple and sensitive extractive spectrophotometric methods have been described for the assay of Tamsulosin hydrochloride either in pure form or in pharmaceutical formulations. The developed methods involve formation of colored chloroform extractable ion-pair complexes of the drug with bromothymol blue (BTB), bromophenol blue (BPB), bromocresol purple (BCP) and bromocresol green (BCG) in acidic medium. The extracted complexes showed absorbance maxima at 415 nm for all four methods. Beer's law is obeyed in the concentration ranges 2.5-25, 2.5-25, 2.0 - 25 and 2.5 - 25. μg/ml with BTB, BPB, BCP and BCG, respectively. The effect of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined for four methods. All the four methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.

Keywords

Tamsulosin Hydrochloride, Bromothymol Blue, Bromophenol Blue, Bromocresol Green, Bromocresol Purple, Spectrophotometry.

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M. Seethamma ¹, B. Vijayakumar ¹, T. Sai Prasad ¹, G. Venkateshwarlu ¹

Affiliations
1 Department of Chemistry, Nizam College (O.U), Hyderabad, 500001, IN

Abstract

Three simple and sensitive extractive spectrophotometric methods have been described for the assay of Moxifloxacin hydrochloride either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with bromothymol blue (BTB), bromophenol blue (BPB) and bromocresol green (BCP) in acidic medium. The extracted complexes showed absorbances maxima at 415,416 and 417nm with BTB, BPB and BCG respectively. Beer's law is obeyed in the concentration ranges 2.5-25μg/ml for all three dyes. The effect of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined for three methods. All the three methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.

Keywords

Moxifloxacin Hydrochloride, Bromothymol Blue, Bromophenol Blue, Bromocresol Green, Spectrophotometry.

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K. Damodar Reddy ¹, Sayaji Rao ¹, G. Venkateshwarlu ¹

Affiliations
1 Department of Chemistry, Nizam College, Hyderabad, 500 001, IN

Abstract

Six drugs viz., Metoprolol tartrate, Torsemide, Diclofenac sodium, Chlorpheniramine, Loratadine and irbesartan were tested for the formation of charge transfer complexes with p-CA and the interaction formed a basis for quantitative determination of the drugs spectrophotometrically. CH₃CN was found to be suitable for the analysis. The methods have been validated in terms of ICH guidelunes The complexes were found to have 1:1 composition and have stability of the order 10⁵.

Keywords

Spectrophotometry, p-CA, Drug, Quantification, Validation.

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M. Chennaiah ¹, T. Veeraiah ², T. Charan Singh ¹, G. Venkateshwarlu ¹

Affiliations
1 Department of Chemistry, Nizam College (O.U), Hyderabad, 500001, IN
2 Department of Chemistry, SAP College, Vikarabad, Ranga Reddy, A P, 501101, IN

Abstract

Three simple and sensitive extractive spectrophotometric methods have been described for the assay of Pioglitazone hydrochloride either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with bromothymol blue (BTB), bromophenol blue (BPB) and bromocresol purple (BCP) in acidic medium. The extracted complexes showed absorbance maxima at 415 nm for all three methods. Beer's law is obeyed in the concentration ranges 2.0-30, 3.0-30 and 2.5-25μg/ml with BTB, BPB and BCP respectively. The effect of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined for three methods. All the three methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.

Keywords

Pioglitazone Hydrochloride, Bromothymol Blue, Bromophenol Blue, Bromocresol Purple, Spectrophotometry.

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Ravinder Burugu , G. Venkateshwarlu

Abstract

A simple, specific, accurate and precise high performance liquid chromatographic method was developed for the determination of Amlodipine and Atorvastatin in Amlodipine besylate and Atorvastatin tablet dosage forms. A Hypersil BDS C₁₈ 5 μm (250×4.6 mm I.D) with column temperature 30°C in isocratic mode, with mobile phase containing Acetonitrile:10 mM Ammonium acetate buffer pH 4.0 (50:50) was used. The flow rate was 1.0 mL/min and effluent was monitored at 238 nm.The retention time of Amlodipine was 2.5 min and for Atorvastatin was 4.7 min. The linearity for Amlodipine and Atorvastatin was in the range 0.02 mg/mL to 0.12 mg/mL. The proposed method is accurate, precise, specific and rapid estimation of Amlodipine and Atorvastatin in Amlodipine besilate and Atorvastatin tablets.

Keywords

HPLC, Amlodipine Besylate (AMD), Atorvastatin (ATVR), Dissolution, Assay, Validation, Isocratic Elution.

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Jagjitsingh C. Girase ¹, G. Venkateshwarlu ², Saurabh Kumar Banerjee ¹

Affiliations
1 School of Pharmacy Technology and Management, SVKM’s, NMIMS University, Shirpur Campus, Babulde, Near Bank of Tapi River, Shirpur-425405, Dist Dhule (M.S), IN
2 Research and Development Division, Concept Pharmaceuticals Ltd, Aurangabad, IN

Abstract

A reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Ceftriaxone Sodium and Sulbactam Sodium in bulk and Pharmaceutical Formulation. The separation was done by a Kromasil C18 column (250 mm×4.6 mm, 5 μm) using Acetonitrile: Buffer PH 7: Distilled Water (400:50:550, v/v/v) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limit of detection was approximately 0.39 μg/ml for Ceftriaxone Sodium and 0.08 μg/ml for Sulbactam sodium. Due to simplicity and accuracy the method particularly suitable for routine pharmaceutical quality control.

Keywords

RP-HPLC, Ceftriaxone Sodium, Sulbactam Sodium.

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