Asian Journal of Research in Chemistry

Abstract

A simple, rapid, accurate, precise and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of Efavirenz in bulk and in pharmaceutical formulations. The quantification was carried out using Luna column C₁₈ (250 ×4.6mm i.d., 5μm particle size) column in an isocratic mode, with mobile phase comprising a mixture of Methanol, Water and Tetrahydrofuran in a ratio of 30:50:20 (% v/v/v). The flow rate was 1.0 ml/min and the detection was carried out at 259 nm. The retention time of the drug was found to be 3.675 min and the method produced linear response in the concentration range of 20-60 μg/ml (r=0.9993). The recovery studies were also carried out and % RSD from reproducibility was found to be 0.86. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Efavirenz in tablets.

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Abstract

The objective of the study is to develop a reversed phase high performance liquid chromatographic method using ultra violet detection for the determination of Dobutamine HCl in bulk and pharmaceutical dosage forms. Phenomenex Luna C₁₈ column (250 mm x 4.6 mm, 5μ) was used as the stationary phase with a mixture of Acetonitrile:Methanol:Tetrahydrofuran (70:20:10 v/v/v) as the mobile phase. The response of the drug was linear in the concentration range of 10-30 μg/ml. Limit of detection and Limit of Quantification were found to be 0.34 and 1.1 μg/ml, respectively. The percentage recovery ranged between 99.54 and 100.06%. The factors affecting column separation of the analyte were studied. The results demonstrated that this method is reliable, reproducible and suitable for routine analysis.

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