Asian Journal of Research in Chemistry

T. C. Borole ¹, M. G. Dewani ¹, S. P. Gandhi ¹, M. C. Damle ¹

Affiliations
1 Department of Quality Assurance, AISSMS College of Pharmacy, Pune - 411001, Maharashtra, IN

Abstract

A Simple, fast and precise reverse phase high performance liquid chromatographic method is developed for the simultaneous determination of Rosuvastatin Calcium and Fenofibrate. Chromatography was carried out at 40°C and Separation of these drugs were performed on BDS C18 column (250 x 4.6 mm, 5 μ) as stationary phase with a mobile phase comprising of Acetonitrile: water (pH adjust to 2.5 with O-Phosphoric Acid) in the ratio of 70:30 (v/v) at a flow rate of 1.5 ml/min and Detection wavelength of Rosuvastatin Calcium and Fenofibrate ware 287nm. The Retention time for Rosuvastatin Calcium and Fenofibrate were 2min and 8.5min respectively. The linearity of Rosuvastatin Calcium and Fenofibrate was in the range of 1 to 5 μg/mL. The recovery was calculated by standard addition method. The proposed method was found to be accurate, precise and rapid for simultaneous determination of Rosuvastatin Calcium and Fenofibrate. It can be used for routine analysis.

Keywords

Rosuvastatin Calcium, Fenofibrate, RP-HPLC, Validation.

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R. K. Godge ¹, M. C. Damle ¹, S. R. Pattan ¹, P. N. Kendre ¹, S. N. Lateef ¹, P. J. Burange ¹

Affiliations
1 Pravara Rural College of Pharmacy, Pravaranagar, Dist. Ahemadnagar 413 736. (MS), IN

Abstract

A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed for the simultaneous estimation of Pseudoephidrine sulphate and Desloratidine from pharmaceutical formulation by using internal standard Nabumetone. The method was carried out on a HiQ sil C18 W (250mm x 4.6 mm) column, with a mobile phase consisting of Acetonitrile: Methanol :Ammonium acetate (45:40:15 v/v) at a flow rate of 1.5 ml/min. Detection was carried out at 265 nm. The retention time of Pseudoephidrine sulphate, Desloratidine, and Nabumetone were 2.067, 3.050 and 3.750min. Respectively. The developed method was validated in terms of accuracy, precision, linearity, Limit of detection, Limit of quantitation. The proposed method can be used for estimation of these drugs in combined dosage form for routine analysis.

Keywords

Pseudoephidrine Sulphate, Desloratidine, Nabumetone, RP-HPLC.

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