Asian Journal of Research in Chemistry

M.A. Haneef ¹, B. Rajkamal ¹, G. Tharun goud ², V. Mohan Goud ²

Affiliations
1 Ganga Pharmacy College, Nizamabad, A.P, IN
2 University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of zidovudine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of, methanol and phosphate buffer (65:35 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 264nm. The retention time of the drug was 2.55±0.02min.The method produce linear responses in the concentration range of 20-80mg/ml of zidovudine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations

Keywords

Zidovudine, RP-HPLC, Validation

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