Asian Journal of Research in Chemistry

Tentu Nageswara Rao ¹, Muralidhar Reddy Avuthu ², B. Venkata Reddy ³, S. N. V. S. Murthy ⁴

Affiliations
1 Department of Chemistry, Krishna University, Machilipatnam, A.P., IN
2 Department of Chemistry, SVKP & Dr. K.S Raju Arts & Science College, Penugonda, A.P, IN
3 Department of Animal Science, Chonbuk National University, Jeonju, KP
4 Department of Chemistry, DLR PG College, G. Mamidada, AP, IN

Abstract

A simple and inexpensive method was developed with high performance liquid chromatography with PDA detection for determination of Terbinafine HCl and related impurities. The chromatographic separations were achieved on (150×4.6 mm), 5.0 μm make: YMC-Pack ODS-AM, 120A column employing Acetonitrile: methanol and Water pH 7.5, in the ratio of 20:10:10 v/v/v as mobile phase with gradient programmed at flow rate 1.0 mL/min was chosen. Three impurities were eluted within 35 minutes. The detector wavelength of 230 nm was employed.

Keywords

Forced Degradation, HPLC, Method Validation, Terbinafine.

Full Text