Asian Journal of Research in Chemistry

Chetan Ramolia ¹, Zarna Dedania ², Ronak Dedania ², N. R. Sheth ³, G. Vidya Sagar ², Bhavna Patel ⁴, K. K. Bhatt ⁵

Affiliations
1 CRO, Veeda, Ahmedabad, IN
2 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, IN
3 Department of Pharmaceutical Sciences, Saurastra University, Rajkot, Gujarat, IN
4 CVM Institute for Degree Course in Pharmacy, New Vallabh Vidhyanagar, IN
5 Ipcowala Indukaka College of Pharmacy, Vallabh Vidhyanagar, IN

Abstract

A simple, specific, accurate and stability indicating reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of and Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in tablet dosage forms. Detector consists of photodiode array detector; the reversed phase column used was RP-C18 (2.27 μm size, 250 mm´4.6 mm i.d.) at ambient temperature, in isocratic mode, with mobile phase containing Methnol: Acetonitrile: Phosphate Buffer, pH 5.39 (20: 40: 40% v/v/v) adjusted to pH 5.39 using ortho phosphoric acid was used. The flow rate was 1.0 ml/min and effluents were monitored at 238 nm. The retention times of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride were 3.69 min, 8.18 min and 12.5 min respectively.

The calibration curves were linear in the concentration range of 4-24 μg/ml for Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride. The proposed method was validated and successfully applied to the estimation of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in combined tablet dosage forms. Linearity was obtained in the concentration range of 4 to 24 μg/ml of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride with a correlation coefficient of 0.9963, 0.9992 and 0.9957 respectively. Detector consists of photodiode array detector; the reversed phase column used was RP-C18 (2.27 μm size, 250 mm, 4.6 mm i.d.) at ambient temperature. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride in routine analysis.

Keywords

Metformin Hydrochloride, Rosiglitazone Maleate and Glimepiride, RP-HPLC.

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Zarna Dedania ¹, Ronak Dedania ¹, Vaishali Karkhanis ², G. Vidya Sagar ¹, Meeta Baldania ¹, N. R. Sheth ³

Affiliations
1 Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch, Gujarat, IN
2 A.R. College of Pharmacy, Vallabh, Vidhyanagar, Gujarat, IN
3 Department of Pharmaeutical Sciences, Saurastra University, Rajkot, Gujarat, IN

Abstract

A RP-HPLC method is developed for simultaneous estimation of omeprazole and ondansetron in combined tablet dosage form. Omeprazole is a proton pump inhibitor and in the treatment of gastro-oesophageal reflux disease (GERD), peptic ulcer and Zollinger-Ellison syndrome. Ondansetron is used as selective 5-HT3 receptor antagonist and used in the management of nausea and vomiting induced by cytotoxic chemotherapy and radio therapy and also post operative nausea and vomiting. The mobile phase used was a combination of Methanol: Acetonitrile (90:10). The detection of the combined dosage form was carried out at 218 nm and a flow rate employed was 0.5 ml/min. The retention time for omeprazole and ondansetron was found to be 5.39 and 11.08 min respectively. Linearity was obtained in the concentration range of 4 to 20 μg/ml of omeprazole and 4 to20 μg/ml of ondansetron with a correlation coefficient of 0.997 and 0.9967. Detector consists of photodiode array detector; the reversed phase column used was RP-C18 (2.27μm size, 250 mm´4.6 mm i.d.) at ambient temperature. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of ondansetron and omeprazole in routine analysis.

Keywords

Simultaneous Estimation, RP-HPLC, Omeprazole, Ondansetron.

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