Asian Journal of Research in Chemistry

V Bojaraju ¹, D Sireesha ¹, Mahesh Nasare ¹, V.V.L.N. Prasad ¹, Prakash V. Diwan ¹

Affiliations
1 Department of Pharmaceutical Analysis and Quality assurance, Lalitha College of Pharmacy, Venkatapur (V), Ghatkesar (M), R.R Dist., A.P.-501301

Abstract

A simple, rapid and selective RP -HPLC method has been developed for determination of Amoxicillin trihydrate and Tinidazole in the pharmaceutical dosage form. The chromatographic separation was achieved with reverse phase phenomenex ® (Luna 5μ C18(2) 100A (250 × 4.60 mm) Column in isocratic mode and mobile phase containing 25mM Potassium dihydrogen phosphate (adjusted to pH 4.5 by Ortho-phosphoric acid) and Acetonitrile in the ratio of 40:60 % v/v. The flow rate was 1mL min-1 and effluent was monitored at 238 nm. Retention time was found to be 2.053 min for Amoxicillin trihydrate and 2.944 min for Tinidazole , respectively. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 5-50 μg/ml for both Amoxicillin trihydrate and Tinidazole , respectively. Percentage recoveries obtained for Amoxicillin trihydrate and Tinidazole were 100.87% and 99.52% respectively. The proposed method is precise, accurate, selective and rapid for the simultaneous determination of Amoxicillin trihydrate and Tinidazole in pharmaceutical dosage forms.

Keywords

Amoxicillin Trihydrate, Tinidazole , RP-HPLC Method, Validation, Simultaneous Estimation

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P. Mehulkumar ¹, V. Ramesh ², V. Vinay Kumar ³, R. Srinivas ², Prakash V. Diwan ³

Affiliations
1 National Institute of Pharmaceutical Education and Research, Hyderabad-500 037, IN
2 National Centre for Mass Spectrometry, Indian Institute of Chemical Technology, Hyderabad 500 007, IN
3 Pharmacology Division, Indian Institute of Chemical Technology, Hyderabad 500 007, IN

Abstract

A simple, accurate and precise spectroscopic method was developed for simultaneous determination of Amlodipine besylate and Olmesartan medoximil in tablets using first derivative zero crossing method. Amlodipine showed zero crossing point at 237 nm while olmesartan showed zero crossing at 259 nm. The dA/dλ was measured at 259 nm for amlodipine and 237 nm for olmesartan and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear from 5 - 30 μg/mL for amlodipine (r² ≥ 0.9999) at 259 nm and 5 - 30 μg mL-1 for olmesartan (r² ≥0.9998) at 237 nm. The within day and between day variations showed coefficient of variation (CV%) values less than 1.5% for both drugs. The limit of detection was 0.06 and 0.14 μg/mL for amlodipine and olmesartan, respectively. The limit of quantification was 0.18 and 0.43 μg/mL for amlodipine and olmesartan, respectively. The method was successfully applied for simultaneous determination both drug in tablet dosage form.

Keywords

Amlodipine Besylate, Olmesartan Medoximil, Zero Crossing Method, Tablet Dosasge Form.

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