Asian Journal of Research in Chemistry

Sachin U. Kushare ¹, Mahantesh Mali ¹, Atul Phatak ¹, Praveen D. Chaudhari ¹

Affiliations
1 P. E. Society’s Modern College of Pharmacy, Nigdi, Pune 411 044, Maharashtra, IN

Abstract

The simple, selective, rapid, precise and economical spectrophotometric method has been developed for simultaneous estimation of Desloratadine and Pseudoephedrine Hydrochloride, from two components solid dosage forms and dissolution media. Dissolution media 0.1N Hydrochloride acid and 0.1M Potassium Phosphate Buffer P^H 7.5 was used. The simultaneous equation method involves the measurement of absorbance at two wavelength 281.5 nm (λ_max of Desloratadine) and 256.5 nm (λ_max of Pseudoephedrine Hydrochloride) in 0.1N Hydrochloride acid. 241.5 nm (λ_max of Desloratadine) and 256.5 nm (λ_max of Pseudoephedrine Hydrochloride) in 0.1M Potassium Phosphate Buffer (PH 7.5). The linearity lies between 2.5-25 μg/ml for Desloratadine and 120-960 μg/ml for Pseudoephedrine Hydrochloride in both media. The developed method was validated in terms of accuracy, precision, Linearity, Limit of detection, Limit of quantitation. The method showed good reproducibility and recovery with % RSD less than 1.5. The methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of Desloratadine and Pseudoephedrine Hydrochloride from Tablets and dissolution media.

Keywords

Desloratadine, Pseudoephedrine Hydrochloride, Spectrophotometry, Simultaneous Equation Method.

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Pavan P. Chintawar ¹, Poonam N. Pawar ¹, Minal T. Harde ¹, Suhas V. Joshi ², Praveen D. Chaudhari ¹

Affiliations
1 Modern College of Pharmacy, Nigdi, Pune-411044, Maharashtra, IN
2 Modern College of Pharmacy for Ladies, Moshi, Pune-412105, Maharashtra, IN

Abstract

Three simple spectrophotometric methods have been developed for simultaneous estimation of Moxifloxacin HCl and Ketorolac Tromethamine from ophthalmic dosage form. Acidic methanol was used as solvent. Method-I involves, formation of Q-absorbance equation at 307 nm (isoabsorptive point) and 295 nm (λ_max of Moxifloxacin); Method-II simultaneous equation method involves the measurement of absorbance at two wavelength 295 (λ_max of Moxifloxacin) and 320 (λ_max of Ketorolac Tromethamine) and Method-III multicomponent mode of analysis involves measurement of absorbance at two wavelength 295 (λ_max of Moxifloxacin) and 320 (λ_max of Ketorolac Tromethamine);The linearity lies between 1-14 μg/ml for Moxifloxacin and 1-14 μg/ml for Ketorolac Tromethamine for all the three methods. The accuracy and precision of methods were determined and validated statistically. All the method showed good reproducibility and recovery with % RSD less than 1.5. All methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of Moxifloxacin HCl and Ketorolac Tromethamine in bulk and ophthalmic dosage form.

Keywords

Moxifloxacin HCl, Ketorolac Tromethamine, Q-Absorbance Ratio Method, Simultaneous Equation Method.

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