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Hamrapurkar, Purnima D.
- Optimization and Establishment of Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Mefenamic Acid and Paracetamol from Tablet Dosage form
Authors
1 Department of Pharmaceutical Analysis, Prin K.M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaokar Marg, Cuffe Parade, Colaba, Mumbai-400 005
Source
Asian Journal of Research in Chemistry, Vol 6, No 10 (2013), Pagination: 926-931Abstract
A simple, selective and precise gradient HPLC method has been developed for simultaneous estimation of Mefenamic acid and Paracetamol combination from pharmaceutical dosage form. The separation was accomplished on an HiQSil reversed phase C18 column, 250 mm ×4.6mm I.D., 5µm column using two mobile phases i.e. A as methanol: 50mM potassium dihydrogen orthophosphate (pH = 7.5)(20:80 v/v) and B as methanol in a gradient elution mode. The flow rate was 1mL/min. The eluents were monitored with a UV detector set at 245 nm as detection wavelength. The investigated validation elements showed the method has acceptable specificity, accuracy, linearity, precision, robustness and high sensitivity. Detection and quantification limits were established at 0.02 µg/ml and 0.05 µg/ml respectively. A stability indicating HPLC method has been established for analysis of Mefenamic acid and Paracetamol combination. The drug was subjected to stress conditions of hydrolysis, oxidation, photolysis, and thermal decomposition.Keywords
Mefenamic Acid, Paracetamol, Gradient Elution, Stability Indicating Method, HPLC
References
- Maryadele J.O; Patricia E.H, Cherie B.K., Kristin J.R., Catherine M.K., Maryann R.D. The Merck index.14th edition .United states Pharmaceutical Company Merck & co.2006, 5798 page no.1001.
- Sean Sweetman, Martindale: The complete Drug Reference, 36th Edition, Page no.80.
- Pringsheim T, Davenport WJ, Dodick D. Acute treatment and prevention of menstrually related migraine headache: evidencebased review. Neurology. 2008;70(17):1555-1563.
- Sean Sweetman, Martindale: The complete Drug Reference, 36th Edition, Page no.108.
- Hinz, B.; Cheremina, O.; Brune, K. (2008). The FASEB J. Federation of American Societies for Experimental Biology 22 (2): 383–390.
- K.D. Tripathi, “Essentials of Medical pharmacology, 6th-edition 2008.Page No.198-199
- Erdal Dinc¸ Cem Yu¨cesoy, Feyyaz Onur,. J. of Pharm. Biomed. Anal, 28(2002)1091-1100.
- Ali Babaei, Balal Khalilzadeh • Mohammad Afrasiabi, J Appl Electrochem (2010) 40:1537–1543.
- P.D. Sethi. “Quantitative Analysis of Drug in Pharmaceutical Formulation” 2001 Page No. 390-391.
- Harish L. Rau, A.R. Arora and P. Gundu Rao. Indian Drugs, 28(12) 563-565,1991.
- ICH Guidelines: Validation of Analytical Procedures: Test and Methodology, Q2(R1), (2005).
- A Validated and Simplified RP-HPLC of Metoprolol Succinate from Bulk Drugs
Authors
1 Department of Pharmaceutical Analysis, Prin. K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaonkar Marg, Cuffe Parade, Colaba, Mumbai- 400 005, IN
Source
Asian Journal of Research in Chemistry, Vol 2, No 2 (2009), Pagination: 119-122Abstract
A simple, specific, accurate, and precise reverse phase liquid chromatographic method (RP-HPLC) was developed and validated for the estimation of Metoprolol succinate from bulk drugs. A RP Spherisorb C-18 (Waters) 10μm column having 250×4.6 mm ID in isocratic mode, with mobile phase containing acetonitrile: methanol: 10 mM aqueous phosphate buffer (20:20:60%v/v) was used. The flow rate was 1.0 ml/min and effluents were monitored at 254 nm. The retention time of Metoprolol succinate was 5.1 min. The linearity of the method was good (r > 0.998), as also were intra-day and inter-day precision (RSD <2%).
The method was validated for accuracy, specificity, limit of quantification, limit of detection, robustness and stability. The results showed that proposed method is successfully applied for the quantitative determination of Metoprolol succinate in bulk drugs.