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Farag, Rabie S.
- Extractive Spectrophotometric Determination of Mianserin Hydrochloride by Acid-Dye Complexation Method in Pure and in Pharmaceutical Preparations
Authors
1 Al-Azhar University, Nasr City 11884, Cairo, EG
2 Chemical Laboratory of Medico-Legal Department, Ministry of Justice, Cairo, EG
Source
Asian Journal of Research in Chemistry, Vol 4, No 9 (2011), Pagination: 1388-1392Abstract
A simple and sensitive extractive spectrophometric method has been described for the assay of Mianserin hydrochloride (M-HCl) either in pure form or in pharmaceutical solid dosage form. The developed method involves formation of colored chloroform extractable ion-association complex of Mianserin hydrochloride (M-HCl) with Picric acid (PA), Chlorophenol red (ClPR), Bromthymol blue (BrTB), Bromcresol purple (BrCP) reagents. The extracted complexes showed absorbance maxima at optimum wavelength using visible spectrophotometer. Beer's law is obeyed in the concentration range of 1-42 μg/mL. Correlation coefficient was found to be ≥ 0.9985. In addition we have determined the molar absorptivity, Sandell sensitivity and the optimum conditions for quantitative analysis of the investigated drugs.Keywords
Spectrophotometry, Mianserin Hydrochloride, Chloroform, Pharmaceutical Analysis, Ion-Association Complex.
- Confirmatory Method for Determination of 11-Nor-Δ9-Tetrahydrocannabinol-9-Carboxylic Acid in Urine Samples Using Gas Chromatography–Mass Spectrometry (GC/MS)
Authors
1 Prof. of Inorganic Chemistry, Al-Azhar University, Cairo, EG
2 Drugs and Toxicology Expert, Medico-Legal Organization, Cairo, EG
Source
Asian Journal of Research in Chemistry, Vol 4, No 3 (2011), Pagination: 373-376Abstract
Gas chromatography mass spectrometry (GC/MS) analysis of 11-Nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) and the major metabolite of tetrahydrocannabinol in biological samples is reported The THC metabolite obtained by alkaline hydrolysis from urine samples were extracted using mixture of solvents followed by trimethylsilylation. The derivatized extract was submitted to GC/MS analysis of EI-SIM mode. Different factors were studied as pH, temperature and time to establish the best conditions for the determination. The calibration curves of THC-COOH derivatized (THC-COOH-TMS) in urine samples were linear in the concentration range from 10 to 150 ng/ml. The proposed method is able to determine the major metabolite of cannabis derivatives at very low level (10 ng/ml) with high specificity so is analytical procedure can be used as confirmatory method in drug testing of cannabis use.
Keywords
Cannabinoids, GC/MS Analysis, Tetrahydrocannabinol and Biological Samples.- RP-HPLC Determination of Amitriptyline Hydrochloride in Tablet Formulations and Urine
Authors
1 Prof. of Inorganic and Analytical Chemistry, Al-Azhar University, Cairo, EG
2 Forensic Chemist, Medico-Legal Organization, Ministry of Justice, Cairo, EG
Source
Asian Journal of Research in Chemistry, Vol 4, No 1 (2011), Pagination: 24-27Abstract
A new, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of amitriptyline hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50 % v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mm x 4 mm i.d.). The flow rate was 0.6 ml/min and UV detection at 254nm. The retention time for amitriptyline hydrochloride was 7.3 min. The calibration curve was linear in the range 0.5-3 μg/mL. The mean recovery for amitriptyline hydrochloride is 100.025 %. The assay was precise within day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.