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Das, S. K.
- Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200
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Authors
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1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
Source
Asian Journal of Research in Chemistry, Vol 6, No 7 (2013), Pagination: 613-617Abstract
Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.Keywords
Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, Mutant
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