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Authors
Affiliations
1 Department of Pharmaceutical Analysis, Gautham College of Pharmacy (Affiliated to Rajiv Gandhi University of Health Sciences), R.T. Nagar post, Bangalore, Karnataka-560032, IN
2 Karuna College of Pharmacy (Affiliated to Kerala University of Health Sciences), Irringuttur, Thirumittacode P.O. Palakad Dist. Kerala-679533, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 9 (2011), Pagination: 1371-1377
Abstract
A simple, selective, rapid and precise RP-HPLC has been developed for the simultaneous estimation of Metamizole sodium and Pitofenone HCl in presence of Fenpiverinium bromide of combined Pharmaceutical dosage forms. The Inertsil ODS 3V C-18 Column was used for Metamizole sodium and Pitofenone HCL separation. The Mobile phase used was sodium dihydrogen phosphate buffer : Methanol,(0.05M, pH 5.0), (53:47 v/v) at a flow rate of 1ml/min at 286nm. The calibration curves was linear at a concentration range of 700.90 μg/ml to 1301.73 μg/ml and 7.01 μg/ml to 13.01 μg/ml with its regression coefficient (r2=0.9992 and 0.9995) for Metamizole sodium and Pitofenone HCl respectively was obtained. The LOD and LOQ was found to be in the range of 21.6174 μg/ml and 65.507 μg/ml for Metamizole sodium and 0.1701 μg/ml and 0.5155 μg/ml for Pitofenone HCl respectively. The method is highly sensitive and successfully applied for determination of Metamizole sodium and Fenpiverinium bromide in tablet dosage forms.
Keywords
Metamizole Sodium, Pitofenone HCl, Reverse Phase High Performance Liquid Chromatography.
