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Authors
Affiliations
1 Department of Pharmaceutical Chemistry, Manonmaniam Sundaranar University, Tirunelveli – 627 012, Tamil Nadu, IN
2 The Madras Pharmaceuticals, Chennai – 600 096, Tamil Nadu, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 6 (2011), Pagination: 882-886
Abstract
A simple, reproducible and efficient reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for estimation of Clobazam in its tablet dosage form. Separation was done by using mobile phase consists of HPLC Grade Water and Acetonitrile in the ratio of 60:40. Chromatography separations were carried out on prontosil C18 column (150X4.6mn; 5μm) at a flow rate of 1.0 ml/min and UV detection at 230nm and the retention time for clobazam is 10.03 minutes. The linear dynamic response was found to be in the concentration of 80μg- 130μg/ml with correlation coefficient of 0.9976. The percentage recovery of clobazam was found to be 101.64%. The proposed method was found to be simple, accurate, precise and rapid and could be used for routine analysis. This condition was applied only for tablet dosage form. The statistical parameters and recovery studies were carried out and reported.
Keywords
Clobazam, RP-HPLC, Tablets, Validation.
