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Saraf, Swarnlata
- Simple and Rapid Spectrofluorometric Fingerprint Development of Quinine in Unani Formulation
Authors
1 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (Chhattisgarh)- 492010, IN
Source
Asian Journal of Research in Chemistry, Vol 5, No 1 (2012), Pagination: 14-17Abstract
A simple, precise and accurate spectrofluorometric method has been established for quantitative determination of quinine. Conditions were also optimized for best possible extraction of quinine via varying concentrations of different solvents for maximum recovery of quinine at excitation wavelength 372 nm and emission wavelength 456 nm. Methanol found to be best for highest possible recovery of target analyte quinine. The linear range of 1.0- 7.0 μg/mL was obtained for quinine. The detection limit was 0.1768 and quantitation limit was found to be 0.5411for quinine. The recovery of the quinine was found to be 97.35-99.85 %. The proposed method was used for the determination of quinine alkaloids in pharmaceuticals and herbal formulations with satisfactory result.
Keywords
Unani Medicine, Validation, Spectrofluorometer, Quinine.- TLC Densitometry Method for Determination of Cinnamaldehyde in a Traditional Indian Formulation
Authors
1 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492010, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 12 (2011), Pagination: 1953-1956Abstract
A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method developed and validated for the determination of Cinnamaldehyde in Indian traditional formulation (sitopaladi churna) and crude drug extracts. Analysis was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of Toluene: Ethyl acetate: Methanol (8:1:1) at room temperature (25±2°C). Camag TLC scanner III was used for spectrdensitometric scanning and analysis in absorbance mode at 295 nm. The system was found to give compact spots for Cinnamaldehyde (Rf value of 0.55±0.02). The linear regression analysis data for the calibration plots showed good linear relationship (r2 = 0.996±0.0003) in the concentration range 200-1200 ng spot−1 with respect to peak area. According to the International Conference on Harmonization (ICH) guidelines, the method was validated for precision, recovery, robustness and ruggedness. The limits of detection and quantification were determined. The Cinnamaldehde content was quantified and estimated from the formulation and the cinnamomum zeylanicum plant part. Statistical analysis of the data showed that the method is reproducible and selective for the quantitatve determination of Cinnamaldehyde.Keywords
Cinnamaldehyde, High-Performance Thin Layer Chromatography, Indian Traditional Formulation, Quantitative Analysis.- HPLC Determination of Piperine in ‘Trikatu Churna’ a Potent Ayurvedic Formulation for Routine Quality Control
Authors
1 Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (CG), IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 2 (2011), Pagination: 183-186Abstract
Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Ayurvedic medicines/herbal formulations. ‘Trikatu churna’ is one of the oldest and popular Ayurvedic preparations, is official in Ayurvedic formulary of India used widely for disorder of respiratory tract and digestive system. It comprised of the fruits Piper longum (Pippali), Piper nigrum (Marica) and rhizomes of Zingiber officianalis (Saunth). The present study is an attempt to develop the fingerprint method for Trikatu Churna by simple high-performance liquid chromatography (HPLC) determination using Piperine as a standard, which is as an important and major content in formulation. RP- HPLC methods for determination of Piperine from the fruits of Pippali, Marica and Trikatu Churna have been developed. A C 18 LUNA (5 micron 25 cm×4.6 mm) column from Phenomenex in binary gradient mode with mobile phase methanol at flow rate is 1.0ml/min, and effluent was monitored at 342 nm. Validation of the method was done with a view to demonstrate its selectivity, linearity, precision and accuracy. The concentration of Piperine present in raw material was found to be 4.1%± 0.42w/w in marica, and 2.05% ± 0.39w/w in pippali respectively and in three identical laboratory batch of Trikatu Churna name TK-I, TK-II, TK-III, was 2.02%±0.61, 2.23%±0.49, 2.21%±0.53w/w respectively with mean value 2.15%±0.54w/w. The Piperine content of all the three batches is found to be in close proximities with each other. Obtained results were compared with marketed formulations.