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Authors
Affiliations
1 Government College of Pharmacy, Vidyanagar, Karad-415124, Dist Satara, IN
Source
Asian Journal of Research in Chemistry, Vol 2, No 3 (2009), Pagination: 353-356
Abstract
Two sensitive, simple, rapid, accurate and precise UV spectrophotometric methods for simultaneous estimation of Rosiglitazone Maleate (RSGN) and Gliclazide (GLZ) in two component solid dosage forms have been developed. These methods employ the application of simultaneous equation and the absorbance ratio (Q-analysis). A Shimadzu 1700 U.V visible spectrophotometer with 1cm matched quartz cells, and 0.1 N NaOH as solvent were employed in the both methods. Simultaneous equation method involves the formation and solving of simultaneous equations at two wavelengths 245 nm (λmax of RSGN) and 226 nm (λmax of GLZ). Q analysis method is based on the determination of absorbance ratio at two selected wavelengths, one being the isoabsorptive point for the two drugs (235 nm) and other being the absorption maxima of RSGN. Both developed methods obeyed the Beer’s law in the concentration range of 4-32 μg/ml for RSGN and 5-40 μg/ml for GLZ. The precision and accuracy of method were confirmed by repeatability and by recovery studies. Both methods were validated statistically. The method shows good repeatability and recovery with % RSD is less than 2. The method can be successfully employed for the simultaneous estimation of RSGN and GLZ in laboratory prepared mixtures and in commercial tablet preparation.
Keywords
Rosiglitzone, Gliclazide, U.V., Simultaneous Estimation.
