Asian Journal of Research in Chemistry

A. Rama Chandra Reddy ¹, K. Roshna ¹, P. Mohanraj ¹, T. M. Kulanthavel ¹, V. S. Saravanan ¹, V. Ganesh ¹

Affiliations
1 Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Veppampalayam, Erode-638112, TN, IN

Abstract

A new simple, sensitive, precise and economical spectrophotometric method of analysis for a bulk drug was developed and validated. The method employed methanol as solvent. The drug was then estimated at 270 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r²=0.999 in the concentration range 0.5-3.0 μg/ml. The mean value of correlation coefficient, slope and intercept were 0.999, 0.0332 and 0.0002 respectively. The method was validated for precision, accuracy and recovery studies. LOD and LOQ for norethisterone was found to be 0.01987 (μg/ml) and 0.06024 (μg/ml) respectively. The method has been successfully applied in the analysis of marketed formulations.

Keywords

Norethisterone, UV Spectrophotometric Analysis.

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J. Vanitha ¹, A. Saravana Kumar ², M. Ganesh ², V. S. Saravanan ¹

Affiliations
1 Department of Analytical Chemistry, Erode College of Pharmacy, Erode-112, IN
2 Department of Pharmaceutical Bio-Technology, Nandha College of Pharmacy, Erode-52, IN

Abstract

Climbazole is a member of the azole class of anti-mycotic, is often incorporated in several pharmaceutical forms and in shampoo formulation it is known to be effective against fungal infection on the scalp. This paper describes a method to quantify climbazole in shampoo formulation by cylinder plat assay method and the HPLC method. The test organism used for the agar diffusion assay was candida albicans ATCC10231. Three different concentrations of climbazole were used for the diffusion assay. A mean zone diameter was obtained for each concentration. A standard curve was obtained by plotting the three values derived from the zone diameter. A prospective validation of the method showed that the method was linear (r=0.9766), precise (RSD=2.45%) and accurate. The results obtained by the cylinder plate method was statistically evaluated by analysis of variance (ANOVA) and the results obtained indicate that there is no significant different between these two methods.

Keywords

Climbazole Shampoo, Microbiological Assay, Cylinder-Plate, Candida albicans.

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