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Mohan Goud, V.
- Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form
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Authors
Affiliations
1 Ganga Pharmacy College, Nizamabad, A.P, IN
2 University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar
1 Ganga Pharmacy College, Nizamabad, A.P, IN
2 University College of Pharmaceutical Sciences, Palamur University, Mahabubnagar
Source
Asian Journal of Research in Chemistry, Vol 6, No 4 (2013), Pagination: 341-344Abstract
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of zidovudine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of, methanol and phosphate buffer (65:35 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 264nm. The retention time of the drug was 2.55±0.02min.The method produce linear responses in the concentration range of 20-80mg/ml of zidovudine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulationsKeywords
Zidovudine, RP-HPLC, Validation
References
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- Development and Validation of RP-HPLC Method for Assay of Seratrodast in Pharmaceutical Dosage Form
Abstract Views :422 |
PDF Views:2
Authors
Affiliations
1 Department of Pharmaceutical Chemistry, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist. A.P
2 Bhaskar Pharmacy College, Yenkapally, Moinabad, R.R. Dist. A.P
3 Department of Pharmaceutical Chemistry, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist.
1 Department of Pharmaceutical Chemistry, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist. A.P
2 Bhaskar Pharmacy College, Yenkapally, Moinabad, R.R. Dist. A.P
3 Department of Pharmaceutical Chemistry, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist.
Source
Asian Journal of Research in Chemistry, Vol 6, No 8 (2013), Pagination: 749-751Abstract
The present work describes a simple reverse-phase high performance liquid chromatographic method for estimation of the drug Seratrodast in pure and in tablet dosage form. The estimation was carried out on a Thermo Hypersil C 18 (150mm × 4.6 mm i.d., particle size 5μm) column with a mixture of Phosphate buffer, Acetonitrile and Water with a pH 6.8 adjusted with ortho phosphoric acid in the ratio of 50:40:10%v/v. UV detection was performed at 266nm. The retention time was 6.016 min. and the flow rate was 1.0 ml min -1. The calibration curve was linear over the concentration range of 200-600ppm. The LOD and LOQ values were found to be 2.5 and 9.6. The method was validated for accuracy, specificity, limit of quantification, limit of detection, robustness and stability. The results showed that proposed method is successfully applied for the quantitative estimation of Seratrodast in pharmaceutical dosage form.Keywords
Seratrodast, RP-HPLC, Phosphate Buffer and AcetonitrileReferences
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- M A Haneef, B Rajkamal, G Tharun Goud, V. Mohan Goud. Development and Validation by RP-HPLC Method for Estimation of Zidovudine in Bulk and Its Pharmaceutical Dosage Form. Asian J. Research Chem. 6(4): April 2013.