Asian Journal of Research in Chemistry

Arvind B. Karadi ¹, S. Appal Raju ¹, Shobha Manjunath ¹, Venugopal Darak ¹

Affiliations
1 Department of Pharmaceutical Analysis, HKES’s College of Pharmacy, Sedam Road, Gulbarga, Karnataka-585105, IN

Abstract

Three simple, sensitive spectrophotometric methods (Method A, B and C) are developed based on the diazotization of Cefditoren Pivoxil with nitrous acid (NaNo₂/HCl) at cold temperature followed by its coupling in-situ with β napthol (Method A) to yield deep purple coloured chromogen, diphenyl amine (Method B) to yield pink coloured chromogen, BMR (Method C) to yield purple coloured chromogen, exhibiting absorption maxima at 542, 506 and 539nm, respectively. Beer's law was obeyed in the concentration ranges of 20-60, 10-50 and 5-30μg/ml respectively. The coloured chromogen was stable for 3-5hrs. These methods were extended to pharmaceutical formulations and there was no interference from any common excepients which are usually present in tablet dosage formulations. The results of analysis have been validated statistically and by recovery studies.

Keywords

Cefditoren Pivoxil, Spectrophotometry, β Napthol, Diphenyl Amine, BMR.

Full Text

     

Venugopal Darak ¹, Arvind B. Karadi ¹, Md. Arshad ¹, S. Appal Raju ¹

Affiliations
1 Department of Pharmaceutical Analysis, HKES’s College of pharmacy, Sedam Road, Gulbarga, Karnataka-585105, IN

Abstract

Two simple, sensitive spectrophotometric methods (Method A and B) are developed based on the diazotization of Mesalamine with nitrous acid (NaNo2/ HCl) at cold temperature followed by its coupling in-situ with Chromotropic acid (Method A) to yield cherry red coloured chromogen, phloroglucinol (Method B) to yield yellow coloured chromogen, exhibiting absorption maxima at 433nm and 421nm , respectively. Beer's law was obeyed in the concentration ranges of 5-25 and 8-40g/ml respectively. The coloured chromogen was stable for 4hrs. These methods were extended to pharmaceutical formulations and there was no interference from any common excepients which are usually present in tablet dosage formulations. The results of analysis have been validated statistically and by recovery studies.

Keywords

Mesalamine, Spectrophotometry, Chromotropic Acid, Phloroglucinol.

Full Text