Asian Journal of Research in Chemistry

V. P. Godse ¹, M. N. Deodhar ¹, A. V. Bhosale ¹, R. A. Sonawane ¹, P. S. Sakpal ¹, D. D. Borkar ¹, Y. S. Bafana ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, Seth Govind Raghunath Sable College of Pharmacy, Saswad, Pune-412301, IN

Abstract

Two simple, sensitive accurate, precise, rapid and economical methods were developed for the estimation of Ezetimibe and Atorvastatin in two components solid dosage form. First method is based on simultaneous equations and second method is based on absorbance ratio method. Ezetimibe has absorbance maxima at 232.5 nm and Atorvastatin has Absorbance maxima at 246.5 nm in methanol. The linearity was obtained in the concentration range 5-30 mcg/ml for both Ezetimibe and Atorvastatin. In the first method the concentration of the drugs were determined by using simultaneous equations and in second method, concentration of the drugs were determined by using ratio of absorbance at iso-absorptive point and at λ_max of one of the drug. The results of analysis have been validated statistically and by recovery studies.

Keywords

Ezetimibe, Atorvastatin, Simultaneous Equations, Absorbance Ratio, Iso-Absorptive Point.

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V. P. Godse ¹, M. N. Deodhar ¹, A. V. Bhosale ¹, R. A. Sonawane ¹, P. S. Sakpal ¹, D. D. Borkar ¹, Y. S. Bafana ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, Seth Govind Raghunath Sable College of Pharmacy, Saswad, Pune-412301, IN

Abstract

A simple, rapid and selective HPLC method has been developed for quantitation of aceclofenac and paracetamol from bulk drug and pharmaceutical formulations using a mobile phase consisting mixture of methanol and water (70:30 v/v) at the flow rate of 1 mL/min. An ODS C-18 (Intersile 25 cm×4.6 mm, 10 μm.) column was used as stationary phase. The retention time of aceclofenac and paracetamol were 1.8 min. and 2.7 min. respectively. Linearity was observed in the concentration range of 2-50 μg/mL for aceclofenac and 5-50 μg/mL for paracetamol. Percent recoveries obtained for aceclofenac and paracetamol were 100.6 and 100.7 respectively. The proposed method is precise, accurate, selective and rapid for the simultaneous determination of aceclofenac and paracetamol.

Keywords

Aceclofenac, Paracetamol, HPLC Method.

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