Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Sadananda Maiya, P.
- In Vitro Antiproliferative and Hepatoprotective Activity Studies of Momordica cymbalaria
Abstract Views :321 |
PDF Views:0
Authors
Affiliations
1 Dr. P. Sadananda Maiya Centre for Food Science and Research, Bengaluru (Karnataka), IN
2 Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), IN
1 Dr. P. Sadananda Maiya Centre for Food Science and Research, Bengaluru (Karnataka), IN
2 Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), IN
Source
Asian Science, Vol 9, No 1&2 (2014), Pagination: 41-46Abstract
Natural products are well known to exhibit the antiproliferative and hepatoprotective activities. The present study aimed at assessing the potency of hydroalcoholic extracts of fruits, aerial parts and ischolar_mains of Momordica cymbalaria. Antiproiferative activity of the extracts assessed using in vitro cell lines such as MCF-7, HepG2, HeLa, PC3, A549 and Vero cell lines. The ability of extracts to exert toxic insult on cancer cells has been the basis of anticancer activity. The extracts were evaluated for hepatoprotective activity by employing primary rat hepatocytes.In the case of HeLa cells, MCR was found to be most potentially toxic with average CTC50 of 67 μg/ ml. MCR possesses more toxicity to PC3 cells than the others. Potential toxicity towards Vero cell lines was exhibited by MCR with average CTC50 value of 63 μg/ml. The results clearly demonstrate that the extract MCR exert potential anticancer activity. In vitro hepatoprotective activity of the plant extracts was studied by employing primary rat hepatocytes. Our results indicate that the drug Silymarin was found to exhibit 96 per cent protection against Paracetamol induced toxicity in Hep G2 cells at the tested concentration of 250 μg/ml. Among the parts of Momordica cymbalaria, MCA and MCR found to have slightly lesser activity compared to Sylimarin. In paracetamol induced toxicity of primary rat hepatocytes, the drug Silymarin was found to exhibit 85.28 per cent protection against Paracetamol induced toxicity in Primary rat hepatocytes at the tested concentration of 250 μg/ml. MCA was found to exhibit comparatively similar protective power than silymarin.Keywords
Momordica cymbalaria, Antiproliferative, Hepatoprotective, Hydroalcoholic Extract.
References
- Bharathi, D.P., Jegadeesan, M. and Kavimani, S. (2010). Antiulcer activity of aqueous extract of fruits of Momordica cymbalaria Hook f. in Wistar rats. Pharmacognosy Res., 2(1): 58-61.
- Bharathi, L.K., Munshi, A.D., Chandrashekhara, S., Behera, T.K., Das, A.B., John, K.J. and Vishalnath (2011). Cytotaxonomical analysis ofMomordica L. (Cucurbitaceae) species of Indian occurrence. J. Geneti., 90(1): 21-30.
- Fernandes, P.N., Lagishetty, C.V., Panda, V.S. and Naik, S.R. (2007). An experimental evaluation of the antidiabetic and antilipidemic properties of a standardized Momordica charantia fruit extracts. BMC Compl. Altern. Med., 7: 29.
- Francis, D. and Rita, L. (1986). Rapid colourometric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunological Methods, 89: 271-277.
- Osinubi, A.A., Enye, L.A., Adesiyun, A.E. and Ajayi, G.O. (2008). Comparative effects of three herbs and standard hypoglycaemic agents on blood glucose in normoglycaemic, hyperglycemic and alloxan-induced diabetic male rats. Afr. J. Endocrinol. & Metab., 7: 1.
- Pramod, K., Deval, R.G., Lakshmayya and Ramachandra, S.S. (2008). Antioxidant and hepatoprotective activity of tubers of Momordica tuberosa Cogn. against CCl4 induced liver injury in rats. Indian J. Exp. Biol., 46: 510-513.
- Pramod, K., Deval, R.G., Lakshmayya and Ramachandra, S.S. (2011). Nephroprotective and nitric oxide scavenging activity of tubers of Momordica tuberosa in rats. Avicenna J. Med. Biotech., 3(2): 87-93.
- Parvathi, S. and Kumar, V.J. (2002). Studies on chemical composition and utilization of wild edible vegetable Athalakkai (Momordica tuberose). Plant Foods Hum Nutr., 57(3-4): 215-222.
- In Vitro Anticancer and Hepatoprotective Activity Studies of Garcinia xanthochymus
Abstract Views :407 |
PDF Views:0
Authors
Affiliations
1 Dr. P. Sadananda Maiya Centre for Food Science and Research, Jayanagara, Bengaluru (Karnataka), IN
2 Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), IN
1 Dr. P. Sadananda Maiya Centre for Food Science and Research, Jayanagara, Bengaluru (Karnataka), IN
2 Department of Studies and Research in Chemistry, Tumkur University, Tumkur (Karnataka), IN
Source
Asian Science, Vol 9, No 1&2 (2014), Pagination: 56-62Abstract
Natural products are very well known to exhibit anticancer activities. The present study aimed at assessing the potency of various extracts of Garcinia xanthochymus against cancer using in vitro cell lines. MCF 7, HepG2, HeLa, PC3, A549 and Vero cell lines were employed for the assessment. The ability of extracts to exert toxic insult on cancer cells has been the basis of anticancer activity. GxF was fond to be very potentially toxic (80 mg/ml) to HEP G2 cell lines among all the tested extracts. Also, it was found to be the most toxic compared to GxA and GxF whose average CTC50 was found to be 180 and 118 mg/ml, respectively. Among all the tested extracts, GxF was found to be potentially toxic to the MCF 7 cell lines whose CTC50 was found to be73 mg/ml. GxA possesses the CTC50 of 810 mg/ ml. GxR was found to be toxic with average CTC50 of 303 mg/ml. This was followed by GxF and GxA with average CTC50 values of 303 and 456 mg/ml. In vitro hepatoprotective activity of the plant extracts was studied by employing primary rat hepatocytes. The drug silymarin was found to exhibit 85.28 per cent protection against paracetamol induced toxicity in primary rat hepatocytes at the tested concentration of 250 mg/ml. It was found that GxF and GxR were found to have comparatively similar protective power like silymarin. These extract exhibited 83.63 per cent and 79.58 per cent protection against paracetamol induced toxicity in primary rat hepatocytes at the concentration of 200 mg/ml, respectively. GxA did not exhibit considerable activity with 55.61 per cent protection.Keywords
Garcinia xanthochymus, In Vitro, Anticancer, Hepatoprotective, Cell Lines.References
- Ambasta, S.P. (1986). The useful plants of India; Publications and Information Directorate, Council of Scientific and Industrial Research: New Delhi, 231pp.
- Baggett, S., Protiva, P., Mazzola, E.P., Yang, H., Ressler, E.T., Basile, M.J., Weinstein, I.B. and Kennelly, E.J. (2005). Bioactive benzophenones from Garcinia xanthochymus fruits. J. Nat. Prod., 68(3): 354-360.
- Baslas, R.K. and Kumar, P. (1981). Isolation and characterisation of biflavanone and xanthones in the fruits of Garcinia xanthochymus. Acta Ciencia Indica Chem., 7(1): 31-34.
- Bawa, A.S. and Khanum, F. (2003). Nutraceuticals: Mechanism of action. Indian Food Industry, 22(6): 44-51.
- Bennett, G.J. and Lee, H.H. (1989). Xanthones from Guttiferae. Phytochemistry, 28(4): 967-998.
- Canter, P.H., Thomas, H. and Ernst, E. (2005). Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends in Biotechnol., 23: 180-185.
- Chandalia, M., Garg, A., Lutjohnn, D., Von Bergmann, K., Grundy, S.M. and Brinkley, L.J. (2000). Beneficial effects of high dietary fibre intake in patients with type II diabetes mellitus. New England J. Med., 342: 1392-1398.
- Chanmahasathien, W., Li, Y., Satake, M., Oshima, Y., Ishibashi, M., Ruangrungsi, N. and Ohizumi, Y. (2003a). Prenylated xanthones fromGarcinia xanthochymus. Chem. Pharm. Bull. Tokyo, 51(11) : 1332-1334.
- Chanmahasathien, W., Li, Y., Satake, M., Oshima, Y., Ruangrungsi, N. and Ohizumi, Y. (2003b). Prenylated xanthones with NGF-Potentiating activity from Garcinia xanthochymus. Phytochemistry, 64(5): 981-986.
- Chen, Y., Fan, H., Yang,G.Z., Jiang, Y., Zhong, F.F. and He, H.W. (2010). Prenylated xanthones from the bark of Garcinia xanthochymus and their 1, 1'-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. Molecules, 15(10): 7438-7449.
- Das, P., Goswami, K., Chinniah, S., Panda, A., Banerjee, N., Sahu, S. and Achari, N.P.B. (2007). Wood for dia fruticosa: traditional uses and recent findings. J. Ethnopharmacol., 110: 189-199.
- Duthie, G.G. and Brown, K.M. (1994). Reducing the risk of cardiovascular disease, published in functional foods, designer foods, pharma foods and nutraceutical. Chapman and Hall Publishers, New York, USA (Ed.) Israel Goldberg. 78-85pp.
- Facciola, S. (1998). Cornucopia II: A source book of edible plants; Kampong Publications: California, 79pp.
- Faisal, Z.A.M., Sotheeswaran, S. and Wijesundera, C. (1982). Economically useful plants of Sri Lanka. Part V. Seed fats of some Garcinia species (Guttiferae), ibid. J. Natl. Sci. Counc. Sri Lanka. 10: 221-223.
- Favero, J., Corbeau, P., Nicolas, M., Benkirane, M., Trave, G., Dixon, J.F.P., Aucouturier, P., Rasheed, S., Liautard, J.P.,
- Devaux, C. and Dornand, J. (1993). Inhibition of human immuno deficiency virus infection by the lectin Jacalin and by a derived peptide showing a sequence similarity with gp120. European J. Immunol., 23: 179-185.
- Gustafson, K.R., Blunt, J.W., Munro, M.H.G., Fuller, R.W.,
- McKee, T.C., Cardellina, J.H., McMahon, J.B., Cragg, G.M. and Boyd, M.R. (1992). The guttiferones, HIV-inhibitory benzophenones fromSymphonia globulifera, Garcinia livingstonei, Garcinia ovalifolia and Clusia rosea. Tetrahedron, 48: 10093-10102.
- Gutierrez, R.M.P., Mitchell, S. and Solis, R.V. (2008). Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J. Ethnopharmacol., 117: 1-27.