Molecular Analysis of Genetic Stability in In vitro Regenerated Plants of Broccoli (Brassica oleracea L. var. italica)

Genetic integrity of tissue culture-raised plantlets of broccoli cv. Solan green head from India was assessed using RAPD. First, highly efficient, reliable and highfrequency shoot regeneration was achieved in leaf (62.96%) and petiole (91.11%) explants on MS medium containing 4.5 mg/l BAP + 0.019 mg/l NAA, and 4.0 mg/l BAP and 0.5 mg/l NAA respectively. Maximum ischolar_maining ability (93.99%) with healthy and vigorous ischolar_mains was observed on MS medium containing 0.20 mg/l NAA. The regenerated plantlets with welldeveloped shoot and ischolar_main system were acclimatized successfully. For genetic stability studies, a total of 66 amplicons were amplified using 15 informative primers with a high degree of monomorphism (88.45%) across the mother plant and 20 randomly selected in vitro regenerated plantlets.