Functional Characterization of Alcohol Dehydrogenase from Crabtree-Negative Yeast Komagataella (Pichia) pastoris

Alcohol dehydrogenases (ADHs) are fermentative enzymes involved in the inter-conversion of acetaldehyde and ethanol; they are present in multiple copies in Crabtree-positive yeasts. Some have a very narrow substrate range such as acetaldehyde and ethanol, others have a broader substrate range which includes medium and long chain aldehydes and alcohols. They have also been reported in Crabtree-negative yeasts such as Kluyveromyces lactis, Scheffersomyces stipitis, Wickerhamomyces anomalus, Ogataea angusta, Komagataella (Pichia) pastoris, etc.