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Optical Nanoscopy Tools for Biologists:Advancements of Fluorophores and Optics for High Resolution and Live Imaging


Affiliations
1 Mechanobiology Institute, National University of Singapore, 5A, Engineering Drive 1, 117411, Singapore
2 RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Japan
3 Laboratory of Molecular Biophysics, Institute for Protein Research, Osaka University, Osaka Prefecture 5650871, Japan
 

Optical nanoscopy has emerged as an important tool for live cell imaging at nanoscale resolution in the field of life sciences. The 2014 Nobel Prize in Chemistry for this invention proves its importance in multidisciplinary areas of science. Several optical nanoscopic methods have been introduced in the past decade to achieve diffraction-unlimited resolution by implementing new optical set-ups or utilization of unique photoswitchable fluorophores, or both. In this review we extensively discuss the biological importance of nanoscopy, and the latest advancements and types of fluorophores needed for imaging. This review will be a starter-kit for biologists working in the field of bioimaging.

Keywords

Fluorophores, Live Cell Imaging, Optical Nanoscopy, Optical Set-Up.
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  • Optical Nanoscopy Tools for Biologists:Advancements of Fluorophores and Optics for High Resolution and Live Imaging

Abstract Views: 424  |  PDF Views: 134

Authors

Dhermendra K. Tiwari
Mechanobiology Institute, National University of Singapore, 5A, Engineering Drive 1, 117411, Singapore
Manisha Tiwari
RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Japan
Bikash R. Sahoo
Laboratory of Molecular Biophysics, Institute for Protein Research, Osaka University, Osaka Prefecture 5650871, Japan

Abstract


Optical nanoscopy has emerged as an important tool for live cell imaging at nanoscale resolution in the field of life sciences. The 2014 Nobel Prize in Chemistry for this invention proves its importance in multidisciplinary areas of science. Several optical nanoscopic methods have been introduced in the past decade to achieve diffraction-unlimited resolution by implementing new optical set-ups or utilization of unique photoswitchable fluorophores, or both. In this review we extensively discuss the biological importance of nanoscopy, and the latest advancements and types of fluorophores needed for imaging. This review will be a starter-kit for biologists working in the field of bioimaging.

Keywords


Fluorophores, Live Cell Imaging, Optical Nanoscopy, Optical Set-Up.



DOI: https://doi.org/10.18520/cs%2Fv112%2Fi04%2F714-724