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Development of a New in Ovo Model for the Assessment of Nephrotoxicity and its Comparison with an Existing in Vivo Model


Affiliations
1 Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi 835 215, India
2 Department of Veterinary Pathology, Ranchi Veterinary College, Birsa Agricultural University, Kanke, Ranchi 834 006, India
 

In this study, the chick embryo model has been used as an alternative to the mammalian model for assessing nephrotoxicity. Resemblance of the chick embryo to mammals in having metanephric kidney has prompted us to explore the possibility of using this system for the assessment of kidney toxicity caused mainly by nephrotoxic drugs, namely gentamicin, cisplatin and doxorubicin. Fertilized hens' eggs incubated at 38°±2°C and 58%-60% relative humidity, were injected with the above-mentioned drugs on embryonic day 11. A significant decrease in the uric acid level of amniotic fluid was shown by embryos of cisplatin and doxorubicin-treated groups when compared to control. Embryos of the gentamicin-treated group showed significant increase in urea concentration. Alterations in uric acid, urea and creatinine levels were found in all groups. Histopathological study showed nephron degeneration. Similar results were found in a mouse model.

Keywords

Chick Embryo, Kidney Cells, Mammalian Model, Nephrotoxic Drugs.
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  • Development of a New in Ovo Model for the Assessment of Nephrotoxicity and its Comparison with an Existing in Vivo Model

Abstract Views: 365  |  PDF Views: 119

Authors

Joyani Das
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi 835 215, India
Dinakar Sasmal
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi 835 215, India
Papiya Mitra Mazumder
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi 835 215, India
Kaushal Kumar Singh
Department of Veterinary Pathology, Ranchi Veterinary College, Birsa Agricultural University, Kanke, Ranchi 834 006, India

Abstract


In this study, the chick embryo model has been used as an alternative to the mammalian model for assessing nephrotoxicity. Resemblance of the chick embryo to mammals in having metanephric kidney has prompted us to explore the possibility of using this system for the assessment of kidney toxicity caused mainly by nephrotoxic drugs, namely gentamicin, cisplatin and doxorubicin. Fertilized hens' eggs incubated at 38°±2°C and 58%-60% relative humidity, were injected with the above-mentioned drugs on embryonic day 11. A significant decrease in the uric acid level of amniotic fluid was shown by embryos of cisplatin and doxorubicin-treated groups when compared to control. Embryos of the gentamicin-treated group showed significant increase in urea concentration. Alterations in uric acid, urea and creatinine levels were found in all groups. Histopathological study showed nephron degeneration. Similar results were found in a mouse model.

Keywords


Chick Embryo, Kidney Cells, Mammalian Model, Nephrotoxic Drugs.



DOI: https://doi.org/10.18520/cs%2Fv112%2Fi05%2F965-972