Current Science

Open Access Open Access  Restricted Access Subscription Access

Evaluation of the Genetic Diversity and Population Structure in Drumstick (Moringa oleifera L.) Using SSR Markers


Affiliations
1 M.S. Swaminathan Research Foundation, 3rd Cross Street, Institutional Area, Taramani, Chennai 600 113, India
 

Moringa belongs to the family Moringaceae comprising 13 species of which Moringa oleifera is more widely cultivated. It is an economically important multipurpose tree with immense nutritional value and has significant potential to address malnutrition. In the present study, a total of 97 accessions collected from different districts of Tamil Nadu, Andhra Pradesh and Odisha were genotyped using 20 simple sequence repeat (SSR) markers to assess the genetic diversity and population structure. A total of 140 alleles were detected with the polymorphic information content value of 0.6832 and gene diversity 0.7292. Population structure analysis through a model- based approach divided the accessions into two subgroups. Molecular variance analysis using principal coordinate analysis (PCoA) summarized a 18.32% variation in the first 3 axes and analysis of molecular variance analysis indicates a 2% variance among the population with the remaining 98% variance attributed to variation within individuals. Cluster analysis based on unweighted neighbour-joining showed a clear separation of samples into two subgroups. Further comparison of the cluster subgroup showed high consistency with the STRUCTURE pattern and PCoA plot. The findings reveal a high diversity in the analysed genotypes from which a few distinct accessions could be utilized for further exploration based on their nutritional content and for conservation of nutritionally superior germplasm.

Keywords

Genetic Diversity, Moringa oleifera, Population Structure, SSR.
User
Notifications
Font Size

  • Ramachandran, C., Peter, K. V. and Gopalakrishnan, P. K., Drumstick (Moringa oleifera): a multipurpose Indian vegetable. Econ. Bot., 1980, 34, 276–283.

  • Jyothi, P. V., Atluri, J. B. and Reddi, C. S., Pollination ecology of Moringa oleifera (Moringaceae). Proc: Plant Sci., 1990, 100, 33–42.

  • Muluvi, G. M. et al., Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam. Mol. Ecol., 1999, 8, 463–470.

  • Pandey, A., Pradheep, K., Gupta, R., Nayar, E. R. and Bhandari, D. C., Drumstick tree (Moringa oleifera Lam.): a multipurpose potential species in India. Genet. Resour. Crop Evol., 2011, 58, 453–460.

  • Fahey, J. W., Moringa oleifera: a review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees Life J., 2005, 1, 1–15.

  • Ndabigengesere, A. and Narasiah, K. S., Influence of operating parameters on turbidity removal by coagulation with Moringa oleifera seeds. Environ. Technol., 1996, 17, 1103–1112.

  • Varshney, R. K., Graner, A. and Sorrells, M. E., Genic microsatellite markers in plants: features and applications. Trends Biotechnol., 2005, 23, 48–55.

  • Agarwal, M., Shrivastava, N. and Padh, H., Advances in molecular marker techniques and their applications in plant sciences. Plant Cell. Rep., 2008, 27, 617–631.

  • Doyle, J. J. and Doyle, J. L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue Phytochem. Bull., 1987, 19, 11–15.

  • Wu, J. C., Yang, J., Gu, Z. J. and Zhang, Y. P., Isolation and characterization of twenty polymorphic microsatellite loci for Moringa oleifera (Moringaceae). Hortic. Sci., 2010, 45, 690–692.

  • Schuelke, M., An economic method for the fluorescent labeling of PCR fragments. Nat. Biotechnol., 2000, 18, 233–234.

  • Liu, K. and Muse, S. V., Powermarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21, 2128–2129.

  • Nei, M., Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci., 1973, 70, 3321–3323.

  • Peakall, R. and Smouse, P. E., Genalex 6.5: genetic analysis in excel. Population genetic software for teaching and research – an update. Bioinformatics, 2012, 28, 2537–2539.

  • Perrier, X. and Jacquemond-Collet, J. P., Darwin software, 2006.

  • Pritchard, J. K., Stephens, M. and Donnelly, P., Inference of population structure using multilocus genotype data. Genetics, 2000, 155, 945–959.

  • Evanno, G., Regnaut, S. and Goudet, J., Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol., 2005, 14, 2611–2620.

  • Earl, D. A., STRUCTURE Harvester: a website and program for visualizing structure output and implementing the evanno method. Conserv. Genet. Resour., 2012, 4, 359–361.

  • Shahzad, U., Khan, M. A., Jaskani, M. J., Khan, I. A. and Korban, S. S., Genetic diversity and population structure of Moringa oleifera. Conserv. Genet., 2013, 14, 1161–1172.

  • Saini, R., Saad, K., Ravishankar, G., Giridhar, P. and Shetty, N., Genetic diversity of commercially grown Moringa oleifera Lam. Cultivars from India by RAPD, ISSR and cytochrome P450-based markers. Plant Syst. Evol., 2013, 299, 1205–1213.

  • Ganesan, S. K., Singh, R., Choudhury, D. R., Bharadwaj, J., Gupta, V. and Singode, A., Genetic diversity and population structure study of drumstick (Moringa oleifera Lam.) using morphological and SSR markers. Ind. Crop Prod., 2014, 60, 316–325.

  • Rufai, S., Hanafi, M., Rafii, M., Ahmad, S., Arolu, I. and Ferdous, J., Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker. BioMed. Res. Int., 2013, 2013.

  • Hamrick, J., Isozymes and the analysis of genetic structure in plant populations. In Isozymes in Plant Biology (ed. Soltis, D. E.), Springer, Netherlands, 1989, pp 87–105.

  • Ben El Hadj Ali, I., Guetat, A. and Boussaid, M., Genetic diversity, population structure and relationships of tunisian Thymus algeriensis Boiss. et Reut. and Thymus capitatus Hoffm. et Link. Assessed by isozymes. Ind. Crop Prod., 2012, 36, 149–163.

  • Padmesh, P., Sabu, K. K., Seeni, S. and Pushpangadam, P., The use of RAPD in assessing genetic variability in Andrographis paniculata Nees, a hepatoprotective drug. Curr. Sci., 1999, 76, 833–835.

  • Singh, R., Narzary, D., Bhardwaj, J., Singh, A. K., Kumar, S. and Kumar, A., Molecular diversity and SSR transferability studies in Vetiver grass (Vetiveria zizanioides L. Nash). Ind. Crop Prod., 2014, 53, 187–198.

  • Mgendi, M. G., Manoko, M. K. and Nyomora, A. M., Genetic diversity between cultivated and non-cultivated Moringa oleifera Lam. Provenances assessed by RAPD markers. J. Cell Mol. Biol., 2010, 8, 95–102.

  • Desplanque, B., Boudry, P., Broomberg, K., Saumitou-Laprade, P., Cuguen, J. and Van Dijk, H., Genetic diversity and gene flow between wild, cultivated and weedy forms of Beta vulgaris L.(Chenopodiaceae), assessed by RFLP and microsatellite markers. Theor. Appl. Genet., 1999, 98, 1194–1201.

  • Li, Z. and Nelson, R. L., RAPD marker diversity among cultivated and wild soybean accessions from four chinese provinces. Crop Sci., 2002, 42, 1737–1744.

  • Prasun, K. D., Bhavani, R. V. and Swaminathan, M. S., A farming system model to leverage agriculture for nutritional outcomes. Agric. Res., 2014, 3, 193–203.


Abstract Views: 400

PDF Views: 137




  • Evaluation of the Genetic Diversity and Population Structure in Drumstick (Moringa oleifera L.) Using SSR Markers

Abstract Views: 400  |  PDF Views: 137

Authors

R. Rajalakshmi
M.S. Swaminathan Research Foundation, 3rd Cross Street, Institutional Area, Taramani, Chennai 600 113, India
S. Rajalakshmi
M.S. Swaminathan Research Foundation, 3rd Cross Street, Institutional Area, Taramani, Chennai 600 113, India
Ajay Parida
M.S. Swaminathan Research Foundation, 3rd Cross Street, Institutional Area, Taramani, Chennai 600 113, India

Abstract


Moringa belongs to the family Moringaceae comprising 13 species of which Moringa oleifera is more widely cultivated. It is an economically important multipurpose tree with immense nutritional value and has significant potential to address malnutrition. In the present study, a total of 97 accessions collected from different districts of Tamil Nadu, Andhra Pradesh and Odisha were genotyped using 20 simple sequence repeat (SSR) markers to assess the genetic diversity and population structure. A total of 140 alleles were detected with the polymorphic information content value of 0.6832 and gene diversity 0.7292. Population structure analysis through a model- based approach divided the accessions into two subgroups. Molecular variance analysis using principal coordinate analysis (PCoA) summarized a 18.32% variation in the first 3 axes and analysis of molecular variance analysis indicates a 2% variance among the population with the remaining 98% variance attributed to variation within individuals. Cluster analysis based on unweighted neighbour-joining showed a clear separation of samples into two subgroups. Further comparison of the cluster subgroup showed high consistency with the STRUCTURE pattern and PCoA plot. The findings reveal a high diversity in the analysed genotypes from which a few distinct accessions could be utilized for further exploration based on their nutritional content and for conservation of nutritionally superior germplasm.

Keywords


Genetic Diversity, Moringa oleifera, Population Structure, SSR.

References





DOI: https://doi.org/10.18520/cs%2Fv112%2Fi06%2F1250-1256