Current Science

Open Access Open Access  Restricted Access Subscription Access

Behaviour of Laboratory-Selected Cry1Ac-Tolerant Strain of Helicoverpa armigera (Hubner) (Lepidoptera:Noctuidae) on Bt-Cotton


Affiliations
1 Department of Entomology, Punjab Agricultural University, Ludhiana 141 004, India
 

The effect of Bacillus thuringiensis toxin Cry1Ac on the behaviour of a laboratory-selected resistant population (72-fold) of Helicoverpa armigera on Bt-cotton was evaluated. Compared with non-Bt-cotton and Cry1Ac toxin incorporated in semi-synthetic diet, resistant larvae reared on Bt-cotton had only 0.13% survival and slower development. The results suggest that Cry1Ac from Bt-cotton exerts a greater toxic effect in terms of larval mortality coupled with decline in larval growth rate compared to semi-synthetic diet.

Keywords

Bt-Cotton, Cry1Ac Toxin, Helicoverpa armigera, Resistant Population.
User
Notifications
Font Size

  • Choudhary, B. and Gaur, K., Biotech cotton in India, 2002 to 2014. ISAAA Series of Biotech Crop Profiles, ISAAA, Ithaca, NY, 2015.

  • Huang, F., Buschman, L. L., Higgins, R. A. and McGaughey, W. H., Inheritance of resistance to Bacillus thuringiensis toxin (Dipel ES) in the European corn borer. Science, 1999, 284, 965–967.

  • Gould, F., Anderson, A., Reynolds, A., Bumgarner, L. and Moar, W., Selection and genetic analysis of a Heliothis virescens (Lepidoptera : Noctuidae) strain with high levels of resistance to Bacillus thuringiensis toxin. J. Econ. Entomol., 1995, 88, 1545–1559.

  • Liu, Y. B., Tabashnik, B. E., Dennehy, T. J., Patin, A. L. and Bartlett, A. C., Development time and resistance to Bt crops. Nature, 1999, 400, 519.

  • Tabashnik, B. E., Evolution of resistance to Bacillius thuringiensis. Annu. Rev. Entomol., 1994, 39, 47–79.

  • Wirth, M. C., Georghiou, G. P. and Federici, B. A., CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus. Proc. Natl. Acad. Sci. USA, 1997, 94, 10536–10540.

  • Frutos, R., Rang, C. and Royer, M., Managing insect resistance to plants producing Bacillus thuringiensis toxins. Crit. Rev. Biotech., 1999, 19, 227–276.

  • Whalon, M. E. and McGaughey, W. H., Bacillus thuringiensis: use and resistance management. In Insecticides with Novel Modes of Action: Mechanism and Application (eds Ishaaya, I. and Degheele, D.), Springer, Berlin, 1998, pp. 106–137.

  • Ojha, A. K. et al., Analysis of resistance to Cry1Ac in fieldcollected pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae), populations. GM Crops Food, 2014, 5(4), 280–286.

  • Kranthi, K. R., Ali, S. and Banerjee, S. K., Resistance to Cry 1AC -endotoxin of Bacillus thuringiensis in a laboratory selected strain of Helicoverpa armigera (Hübner). Curr. Sci., 2000, 78, 1001–1004.

  • Kaur, P. and Dilawari, V. K., Inheritance of resistance to Bacillus thuringiensis Cry1Ac toxin in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) from India. Pest. Manage. Sci., 2011, 67, 1294–1302.

  • Gujar, G. T., Kumari, A., Kalia, V. and Chandrashekar, K., Spatial and temporal variation in susceptibility of the American bollworm, Helicoverpa armigera (Hübner) to Bacillus thuringiensis var. kurstaki in India. Curr. Sci., 2000, 78, 995–1001.

  • Nair, R., Kalia, V., Aggarwal, K. K. and Gujar, G. T., Variation in the cadherin gene sequence of Cry1Ac susceptible and resistant Helicoverpa armigera (Lepidoptera: Noctuidae) and the identification of mutant alleles in resistant strains. Curr. Sci., 2013, 104, 215–223.

  • Tabashnik, B. E., Gassmann, A. J., Crowder, D. W. and Carriere, Y., Insect resistance to Bt crops: evidence versus theory. Nature Biotechnol., 2008 26, 199–202.

  • Liu, F. et al., Evidence of field-evolved resistance to Cry1Acexpressing Bt cotton in Helicoverpa armigera (Lepidoptera: Noctuidae) in northern China. Pest Manage. Sci., 2010, 66, 155–161.

  • Li, G. P., Wu, K. M., Gould, F., Wang, J. K., Miao, J., Gao, X. W. and Guo, Y. Y., Increasing tolerance to Cry1Ac cotton in cotton bollworm, Helicoverpa armigera, was conformed in Bt cotton farming area of China. Ecol. Entomol., 2007, 32, 366–375.

  • Gould, F. and Tabashnik, B. E., Bt-cotton resistance management. In Now or Never, Serious New Plans to Save a Natural Pest Control (eds Mellon, M. and Rissler, J.), Union of Concerned Scientists, Cambridge, MA, 1998, pp. 67–105.

  • Gould, F., Sustainability of transgenic insecticidal cultivars: integrating pest genetics and ecology. Annu. Rev. Entomol., 1998, 43, 701–726.

  • Liu, Y. B. and Tabashnik, B. E., Experimental evidence that refuges delay insect adaptation to Bacillus thuringiensis. In Proc. R. Soc. London, Ser. B, 1997, 264, 605–610.

  • Shelton, A. M., Tang, J. D., Roush, R. T., Metz, T. D. and Earle, E. D., Field tests on managing resistance to Bt-engineered plants. Nature Biotechnol., 2000, 18, 339–342.

  • Kumar, R., Effect of Bt-cotton genotypes and Bt cry gene interaction on the performance of Helicoverpa armigera. M Sc thesis, Punjab Agricultural University, Ludhiana, Punjab, India, 2005.

  • Waldbauer, G. P., The consumption and utilization of food by insects. Adv. Insect Physiol., 1968, 5, 229–282.

  • Tabashnik, B. E., Liu, Y. B., Dennehy, T. J., Sims, M. A., Sisterson, M. S., Biggs, R. W. and Carrière, Y., Inheritance of resistance to Bt toxin Cry1 Ac in a field-derived strain of pink bollworm (Lepidoptera : Gelechiidae). J. Econ. Entomol., 2002, 95, 1018–1026.

  • Ferre, J. and Van Rie, Biochemistry and genetics of insect resistance to Bacillus thuringiensis. Annu. Rev. Entomol., 2002, 47, 501–533.

  • Liu, Y. B., Tabashnik, B. E., Meyer, S. K., Carriere, Y. and Bartlett, A. C., Genetics of pink bollworm resistance to Bacillus thuringiensis toxin Cry1Ac. J. Econ. Entomol., 2001, 94, 248–252.

  • Tabashnik, B. E., Patin, A. L., Dennehy, T. J., Liu, Y. B., Carriere, Y., Sims, M. A. and Antilla, L., Frequency of resistance to Bacillus thuringiensis in field populations of pink bollworm. In Proc. Natl. Acad. Sci. USA, 2000, 97, 12980–12984.

  • Liu, Y. B., Tabashnik, B. E., Dennehy, T. J., Patin, A. L., Sims, M. A., Meyer, S. K. and Carriere, Y., Effects of Bt cotton and Cry1Ac toxin on survival and development of pink bollworm (Lepidoptera : Gelechiidae). J. Econ. Entomol., 2001, 94, 1237–1242.

  • Gould, F., Kennedy, G. G. and Johnson, M. T., Effects of natural enemies on the rate of herbivore adaptation to resistant host plants. Entomol. Exp. Appl., 1991, 58, 1–14.

  • Benry, B. and Denno, R. F., The slow growth–high mortality hypothesis: a test using the cabbage butterfly. Ecology, 1997, 78, 987–999.

  • Peck, S. L., Gould, F. and Ellner, S. P., Spread of resistance in spatially extended regions of transgenic cotton: implications for management of Heliothis virescens (Lepidoptera : Noctuidae). J. Econ. Entomol., 1999, 92, 1–16.

  • Nadaf, A. R. M. and Goud, K. B., Effect of Bt cotton on growth and development of pink bollworm, Pectinophora gossypiella. Indian J. Plant Prot., 2007, 35, 57–59.


Abstract Views: 211

PDF Views: 85




  • Behaviour of Laboratory-Selected Cry1Ac-Tolerant Strain of Helicoverpa armigera (Hubner) (Lepidoptera:Noctuidae) on Bt-Cotton

Abstract Views: 211  |  PDF Views: 85

Authors

Paramjit Kaur
Department of Entomology, Punjab Agricultural University, Ludhiana 141 004, India
Vinod Kumar Dilawari
Department of Entomology, Punjab Agricultural University, Ludhiana 141 004, India

Abstract


The effect of Bacillus thuringiensis toxin Cry1Ac on the behaviour of a laboratory-selected resistant population (72-fold) of Helicoverpa armigera on Bt-cotton was evaluated. Compared with non-Bt-cotton and Cry1Ac toxin incorporated in semi-synthetic diet, resistant larvae reared on Bt-cotton had only 0.13% survival and slower development. The results suggest that Cry1Ac from Bt-cotton exerts a greater toxic effect in terms of larval mortality coupled with decline in larval growth rate compared to semi-synthetic diet.

Keywords


Bt-Cotton, Cry1Ac Toxin, Helicoverpa armigera, Resistant Population.

References





DOI: https://doi.org/10.18520/cs%2Fv112%2Fi07%2F1579-1583