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A 'Six-Step-Strategy' to Evaluate Competence of Plant Growth Promoting Microbial Consortia


Affiliations
1 Plant and Microbial Biotechnology Group, Biotechnology Department, Jaypee Institute of Information Technology, Sector-62, Noida 201 307, India
 

In this study a stepwise, statistically verifiable scientific protocol - 'six-step-strategy' - to develop a consortium is presented. Additionally, it introduces a novel in vivo plant bioassay - 'tube-in-tube' method - that gives faster (< three weeks) and reproducible results for selecting the most desirable consortia combinations. The study employs eight plant growth promoting microbes (PGPMs) with pre-established growth supporting abilities and no mutual antagonism. Following a two-factorial design, 15 consortia combinations (CCs) were developed from these PGPMs. Applying the principles of the 'six-step-strategy', combinations CC11, CC13 and CC4 showing significant increments (> 100%) in ischolar_main length and dry weight were recognized as the best performing consortia. The method thus shortlists the best and manageable number of consortia for further field trials.

Keywords

Bioassay, Consortia Combinations, Plant Growth Promoting Microbes.
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PDF Views: 119




  • A 'Six-Step-Strategy' to Evaluate Competence of Plant Growth Promoting Microbial Consortia

Abstract Views: 421  |  PDF Views: 119

Authors

N. Mishra
Plant and Microbial Biotechnology Group, Biotechnology Department, Jaypee Institute of Information Technology, Sector-62, Noida 201 307, India
S. Krishna Sundari
Plant and Microbial Biotechnology Group, Biotechnology Department, Jaypee Institute of Information Technology, Sector-62, Noida 201 307, India

Abstract


In this study a stepwise, statistically verifiable scientific protocol - 'six-step-strategy' - to develop a consortium is presented. Additionally, it introduces a novel in vivo plant bioassay - 'tube-in-tube' method - that gives faster (< three weeks) and reproducible results for selecting the most desirable consortia combinations. The study employs eight plant growth promoting microbes (PGPMs) with pre-established growth supporting abilities and no mutual antagonism. Following a two-factorial design, 15 consortia combinations (CCs) were developed from these PGPMs. Applying the principles of the 'six-step-strategy', combinations CC11, CC13 and CC4 showing significant increments (> 100%) in ischolar_main length and dry weight were recognized as the best performing consortia. The method thus shortlists the best and manageable number of consortia for further field trials.

Keywords


Bioassay, Consortia Combinations, Plant Growth Promoting Microbes.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi01%2F63-70