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A 'Six-Step-Strategy' to Evaluate Competence of Plant Growth Promoting Microbial Consortia


Affiliations
1 Plant and Microbial Biotechnology Group, Biotechnology Department, Jaypee Institute of Information Technology, Sector-62, Noida 201 307, India
 

In this study a stepwise, statistically verifiable scientific protocol - 'six-step-strategy' - to develop a consortium is presented. Additionally, it introduces a novel in vivo plant bioassay - 'tube-in-tube' method - that gives faster (< three weeks) and reproducible results for selecting the most desirable consortia combinations. The study employs eight plant growth promoting microbes (PGPMs) with pre-established growth supporting abilities and no mutual antagonism. Following a two-factorial design, 15 consortia combinations (CCs) were developed from these PGPMs. Applying the principles of the 'six-step-strategy', combinations CC11, CC13 and CC4 showing significant increments (> 100%) in ischolar_main length and dry weight were recognized as the best performing consortia. The method thus shortlists the best and manageable number of consortia for further field trials.

Keywords

Bioassay, Consortia Combinations, Plant Growth Promoting Microbes.
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  • Rokhzadi, A., Asgharzadeh, A., Darvish, F., Nour-Mohammadi, G. and Majidi, E., Influence of plant growth-promoting rhizobacteria on dry matter accumulation and yield of chickpea (Cicer arietinum L.) under field conditions. Am.-Eurasian J. Agric. Environ. Sci., 2008, 3, 253–257.

  • BCC Research, 2012; retrieved from http://www.bccresearch.com/pressroom/chm/global-market-pesticides-reach-$65.3-billion-2017; last accessed on 2 July 2017.

  • Kholkute, R., Biofertilizers: opportunities and challenges, 2014; http://www.ifaj.org/fileadmin/filedb/a/2014/20141128_RK_Biofertilizers_Opportunities_and_Challenges.pdf (last accessed on 4 September 2015).

  • Ogut, M., Akdag, C., Duzdemir, O. and Sakin, M. A., Single and double inoculation with Azospirillum/Trichoderma: the effects on dry bean and wheat. Biol. Fertil. Soils, 2005, 41, 262–272; doi: 10.1007/s00374-004-0818-3.

  • Singh, S., Singh, B. K., Yadav, S. M. and Gupta, A. K., Potential of biofertilizers in crop production in Indian agriculture. Am. J. Plant Nutr. Fertil. Technol., 2014, 4, 33–40; doi:10.3923/ajpnft.2014.33.40.

  • Nakkeeran, S., Fernando, W. G. D. and Siddiqui, Z. A., Plant growth promoting rhizobacteria formulations and its scope in commercialization for the management of pests and diseases. In PGPR: Biocontrol and Biofertilization (ed. Siddiqui, Z. A.), Springer, Dordrecht, Netherlands, 2010, pp. 257–296.

  • Indian fertilizer scenario. Annual report from the Department of Fertilizers, Ministry of Chemicals and Fertilizers, Government of India, 2013; http://fert.nic.in/sites/default/files/Indian%20Fertilizer% 20SCENARIO-2014.pdf (accessed on 4 September 2015).

  • Majumdar, K., Bio-fertilizer use in Indian agriculture. Indian J. Res., 2015, 4, 377–381.

  • Panneerselvam, P., Selvakumar, G. and Ganeshamurthy, A. N., Microbial consortium – a specialty bioinoculant for sustainable vegetable production. Biofert. New. Lett., 2012, 20, 12–14.

  • Mishra, N. and Sundari, S. K., Native PGPM consortium: a beneficial solution to support plant growth in the presence of phytopathogens and residual organophosphate pesticides. J. Bioprocess. Biotechnol., 2015, 5, 202; doi:10.4172/2155-9821.1000202.

  • Choure, K. and Dubey, R. C., Development of plant growth promoting microbial consortium based on interaction studies to reduce wilt incidence in Cajanus cajan L. var. Manak. World J. Agric. Sci., 2012, 8, 118–128.

  • Mishra, N. and Sundari, S. K., Native PGPMs as bioinoculants to promote plant growth: response to PGPM inoculation in principal grain and pulse crops. Int. J. Agric. Food Sci. Technol., 2013, 4, 1055–1064.

  • Widdel, F., Theory and measurement of bacterial growth. Grundpraktikum Mikrobiol., 2010, 4, 1–11.

  • Long, R. A. and Azam, F., Antagonistic interactions among marine pelagic bacteria. Appl. Environ. Microbiol., 2001, 67, 4975–4983; doi:10.1128/AEM.67.11.4975–4983.2001.

  • Davis, K. E. R., Joseph, S. J. and Janssen, P. H., Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Appl. Environ. Microbiol., 2005, 71, 826–834; doi:10.1128/AEM.71.2.826–834.2005.

  • Rietveld, W. J. and Tinus, R. W., Alternative methods to evaluate ischolar_main growth potential and measure ischolar_main growth. In Proceedings of Intermountain Forest Nursery Association, Oklahoma City, USA, 1987.

  • Yang, C., Yang, L., Yang, Y. and Ouyang, Z., Rice ischolar_main growth and nutrient uptake as influenced by organic manure in continuously and alternately flooded paddy soils. Agric. Water Manage., 2004, 70, 67–81; doi:10.1016/j.agwat.2004.05.003.

  • Kothari, C. R., Research Methodology: Methods and Techniques, Newage International Pvt Ltd Publishers, New Delhi, 2004.


Abstract Views: 434

PDF Views: 128




  • A 'Six-Step-Strategy' to Evaluate Competence of Plant Growth Promoting Microbial Consortia

Abstract Views: 434  |  PDF Views: 128

Authors

N. Mishra
Plant and Microbial Biotechnology Group, Biotechnology Department, Jaypee Institute of Information Technology, Sector-62, Noida 201 307, India
S. Krishna Sundari
Plant and Microbial Biotechnology Group, Biotechnology Department, Jaypee Institute of Information Technology, Sector-62, Noida 201 307, India

Abstract


In this study a stepwise, statistically verifiable scientific protocol - 'six-step-strategy' - to develop a consortium is presented. Additionally, it introduces a novel in vivo plant bioassay - 'tube-in-tube' method - that gives faster (< three weeks) and reproducible results for selecting the most desirable consortia combinations. The study employs eight plant growth promoting microbes (PGPMs) with pre-established growth supporting abilities and no mutual antagonism. Following a two-factorial design, 15 consortia combinations (CCs) were developed from these PGPMs. Applying the principles of the 'six-step-strategy', combinations CC11, CC13 and CC4 showing significant increments (> 100%) in ischolar_main length and dry weight were recognized as the best performing consortia. The method thus shortlists the best and manageable number of consortia for further field trials.

Keywords


Bioassay, Consortia Combinations, Plant Growth Promoting Microbes.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi01%2F63-70