Current Science

Open Access Open Access  Restricted Access Subscription Access

Fluorescence Spectral Features of Blood Components of Pregnant Women


Affiliations
1 Laser Diagnosis of Cancers, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
2 Department of Obstetrics and Gynecology, College of Medicine, King Khalid University Hospital, King Saud University, Riyadh 11451, Saudi Arabia
3 Department of Nuclear Physics, University of Madras, Chennai 600 025, India
4 Cancer Research Chair, College of Medicine, King Saud University, Riyadh 11451, Saudi Arabia
5 College of Medicine, King Saud University, Riyadh 11451, Saudi Arabia
 

During pregnancy, women experience various metabolic and hormonal changes that contribute to foetal development. These changes are investigated in the present study in terms of fluorescent biomolecules found in blood using synchronous fluorescence spectroscopy. Comparing a set of blood samples of 14 pregnant women against age-adjusted controls, it could be seen that the amino acid tryptophan is approximately twofold higher in blood plasma of pregnant women (P < 0.1), while the metabolite flavin adenine dinucleotide is approximately 25% lower. Further, the essential oxygen-carrying protein in the haemoglobin, porphyrin, is 80% higher in pregnant women. When these results were compared with the spectral features of blood components of patients with thalassaemia, it was found that erythrocytes had approximately 25% less haemolysis during the tenure of pregnancy.

Keywords

Fluorescent Biomolecules, Pregnancy, Red Blood Cells, Synchronous Fluorescence Spectra.
User
Notifications
Font Size

  • Margaret, R., Normal hematological changes during pregnancy and the puerperium. In The Obstetric Haematology Manual (eds Pavord, S. and Hunt, B.), Cambridge University Press, Cambridge, UK, 2010, pp. 3–12.

  • Surabhi, C., Anilkumar, T., Sanjay, M., Mohammad, A. and Arvindkumar, V., Physiological changes in hematological parameters during pregnancy. Indian J. Hematol. Blood Transfus., 2012, 28, 144–146.

  • Crocker, I. P., Baker, P. N. and Fletcher, J., Neutrophil function in pregnancy and rheumatoid arthritis. Ann. Rheum. Dis., 2000, 59, 555–564.

  • Shehata, N., Burrows, R. and Kelton, J. G., Gestational thrombocytopenia. Clin. Obstet. Gynecol., 1999, 42, 327–334.

  • Masilamani, V., Al Salhi, M. S., Devanesan, S., Algahtani, F. H., Abu-Salah, K. M., Ahamad, I. and Agastian, P., Spectral detection of sickle cell anemia and thalassemia. Photodiagn. Photodyn. Ther., 2013, 10, 429–433.

  • Devanesan, S. et al., Fluorescence spectral classification of iron deficiency anemia and thalassemia. J. Biomed. Opt., 2014, 19, 027008-14.

  • AlSalhi, M. S., Algahtani, F. H., Devanesan, S., Vijmasi, V. T., Jeyaprakash, K., Alsaeed, A. H. and Masilamani, V., Spectral detection of thalassemia: a preliminary study. J. Biomed. Sci., 2014, 21, 26.

  • Alfano, R. R. and Yang, Y., Stokes shift emission spectroscopy of human tissue and key biomolecules. IEEE J. Quan. Electron., 2003, 9, 148–153.

  • Nirmala, R., Fluorescence spectroscopy of neoplastic and nonneoplastic tissues. Neoplasia, 2000, 2, 89–117.

  • Masilamani, V., Al-Zhrania, K., AlSalhi, M. S., Al-Diabb, A. and Al-Ageily, M., Cancer diagnosis by autofluorescence of blood components. J. Lumin., 2004, 109, 143–154.

  • Kalaivani, R., Masilamani, V., Sivaji, K., Elangovan, M., Selvaraj, V., Balamurugan, S. G. and AlSalhi, M. S., Fluorescence spectra of blood components for breast cancer diagnosis. Photomed. Laser Surg., 2008, 26, 251–256.

  • Liu, Q., Wang, X., Wang, P., Xiao, L. and Hao, Q., Comparison between sonodynamic effect with protoporphyrin IX and haematoporphyrin on sarcoma 180. Cancer Chemother. Pharmacol., 2007, 60, 671–680.

  • Franco, R. S., The measurement and importance of red cell survival. Am. J. Hematol., 2009, 84, 109–114.

  • Giancarlo, L., Francesco, B., Angela, L., Pierluigi, P. and Gina, R., Recommendations for the transfusion of red blood cells. Blood Transfus., 2009, 7, 49–64.

  • Beutler, E., The relationship of red cell enzymes to red cell lifespan. Blood Cells, 1998, 14, 69–91.

  • Berg, J. M., Tymoczko, J. L. and Stryer, L., Haemoglobin transport oxygen efficiency by binding oxygen cooperatively. In Biochemistry, New York, USA, 2002, 5th edn.

  • Pootrakul, P. et al., A correlation of erythrokinetics, ineffective erythropoiesis and erythroid precursor apoptosis in Thai patients with thalassemia. Blood, 2000, 96, 2606–2612.

  • McCurdy, P. R., Mahmood, L. and Sherman, A. S., Red cell life span in sickle cell-hemoglobin C disease with a note about sickle cell-hemoglobin O ARAB. Blood, 1975, 45, 279.


Abstract Views: 321

PDF Views: 116




  • Fluorescence Spectral Features of Blood Components of Pregnant Women

Abstract Views: 321  |  PDF Views: 116

Authors

Sandhanasamy Devanesan
Laser Diagnosis of Cancers, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Mashael AlShebly
Department of Obstetrics and Gynecology, College of Medicine, King Khalid University Hospital, King Saud University, Riyadh 11451, Saudi Arabia
Rudran Kalaivani
Department of Nuclear Physics, University of Madras, Chennai 600 025, India
Krishnan Sivaji
Department of Nuclear Physics, University of Madras, Chennai 600 025, India
Karim Farhat
Cancer Research Chair, College of Medicine, King Saud University, Riyadh 11451, Saudi Arabia
Mohamad Saleh AlSalhi
Laser Diagnosis of Cancers, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Mohamed AlAtawi
Department of Obstetrics and Gynecology, College of Medicine, King Khalid University Hospital, King Saud University, Riyadh 11451, Saudi Arabia
Danny Rabah
College of Medicine, King Saud University, Riyadh 11451, Saudi Arabia
Vadivel Masilamani
Laser Diagnosis of Cancers, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Abstract


During pregnancy, women experience various metabolic and hormonal changes that contribute to foetal development. These changes are investigated in the present study in terms of fluorescent biomolecules found in blood using synchronous fluorescence spectroscopy. Comparing a set of blood samples of 14 pregnant women against age-adjusted controls, it could be seen that the amino acid tryptophan is approximately twofold higher in blood plasma of pregnant women (P < 0.1), while the metabolite flavin adenine dinucleotide is approximately 25% lower. Further, the essential oxygen-carrying protein in the haemoglobin, porphyrin, is 80% higher in pregnant women. When these results were compared with the spectral features of blood components of patients with thalassaemia, it was found that erythrocytes had approximately 25% less haemolysis during the tenure of pregnancy.

Keywords


Fluorescent Biomolecules, Pregnancy, Red Blood Cells, Synchronous Fluorescence Spectra.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi03%2F457-461