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Molecular Characterization and Toxin-Typing of Clostridium difficile Isolates of Dogs and Pigs from Assam and Mizoram of North East India


Affiliations
1 Department of Microbiology, Assam Agricultural University, Khanapara, Guwahati 781 022, India
2 Department of Animal Biotechnology, Assam Agricultural University, Khanapara, Guwahati 781 022, India
3 Department of Biotechnology, Gauhati University, Guwahati 781 014, India
4 Department of Microbiology, Central Agricultural University, Selesih, Aizwal, Mizoram 796 014, India
5 Department of Veterinary Clinical Medicine, Assam Agricultural University, Khanapara, Guwahati 781 022, India
 

Clostridium difficile with its virulence factors A and B toxins cause Pseudomembranous colitis. Bacterium was isolated from 57 dog and 41 pig diarrheic faecalsamples in cycloserinecefaxitin fructose agar media and molecular detection was done by amplification of gluD gene (755 bp). Variability of toxin genes in positive isolates was tested by multiplex PCR. Detection ofbinary toxin genes (cdtA and cdtB) was also done. Results showed 33.67% positivity with 18 and 15 fromdog and pig respectively, from which 10 and 5 were toxigenic and 11 pig isolates exhibited binary toxin. PCR-RFLP demonstrated toxinotype 0 in all A+B+ isolates.

Keywords

Diarrhoea, NE India, Pseudomembranous Colitis, Toxin-Typing, Virulence.
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  • Molecular Characterization and Toxin-Typing of Clostridium difficile Isolates of Dogs and Pigs from Assam and Mizoram of North East India

Abstract Views: 363  |  PDF Views: 131

Authors

B. Das
Department of Microbiology, Assam Agricultural University, Khanapara, Guwahati 781 022, India
R. K. Sharma
Department of Microbiology, Assam Agricultural University, Khanapara, Guwahati 781 022, India
P. Borah
Department of Animal Biotechnology, Assam Agricultural University, Khanapara, Guwahati 781 022, India
S. Das
Department of Biotechnology, Gauhati University, Guwahati 781 014, India
L. M. Barkalita
Department of Animal Biotechnology, Assam Agricultural University, Khanapara, Guwahati 781 022, India
R. K. Mandakini Devi
Department of Microbiology, Central Agricultural University, Selesih, Aizwal, Mizoram 796 014, India
B. C. Baishya
Department of Veterinary Clinical Medicine, Assam Agricultural University, Khanapara, Guwahati 781 022, India

Abstract


Clostridium difficile with its virulence factors A and B toxins cause Pseudomembranous colitis. Bacterium was isolated from 57 dog and 41 pig diarrheic faecalsamples in cycloserinecefaxitin fructose agar media and molecular detection was done by amplification of gluD gene (755 bp). Variability of toxin genes in positive isolates was tested by multiplex PCR. Detection ofbinary toxin genes (cdtA and cdtB) was also done. Results showed 33.67% positivity with 18 and 15 fromdog and pig respectively, from which 10 and 5 were toxigenic and 11 pig isolates exhibited binary toxin. PCR-RFLP demonstrated toxinotype 0 in all A+B+ isolates.

Keywords


Diarrhoea, NE India, Pseudomembranous Colitis, Toxin-Typing, Virulence.

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DOI: https://doi.org/10.18520/cs%2Fv113%2Fi06%2F1099-1106