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In vitro Micropropagation, Total Phenolic Content and Comparative Antioxidant Activity of Different Extracts of Sesbania grandiflora (L.) Pers.


Affiliations
1 Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
2 Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, P.O. Box 524, Auckland Park-2006, South Africa
3 Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, South Africa
4 Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, India
 

Sesbania grandiflora (L.) Pers. is a common traditional medicinal plant used in bronchitis, anaemia,headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism. The present study aimed to assess plant regeneration and plantlets development in vitro using explants of S. grandiflora together with the estimation of total phenolic contentand antioxidative activity of various extracts obtained from the plant. Murashige and Skoog (MS) basal medium added with different concentrations of plant growth regulators (PGRs) was used for plant tissue culture, whereas ferric reducing antioxidant power(FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)assays were used to evaluate the antioxidant potential of different extracts of the plant. In the presence of 6-benzylaminopurine (BAP; 0.1 mg/l), the highest level(85.41%) of seed germination was achieved while the highest callus formation (96.6%) was recorded with 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg/l). Inaddition, the highest shoot induction, shoot formation and shoot elongation were observed with BAP(0.1 mg/l), indole-3-butyric acid (3 mg/l) and naphthaleacetic acid + BAP (0.4 + 0.2 mg/l) respectively.The extract of dried calluses showed highest contents of proline (110.94 mg/g), phenol (16.42 mg/g) and flavonoid (22.22 mg/g), and also highest antioxidant potential with FRAP and DPPH assays. From the present study, we may conclude that the MS basal medium supplemented with PGRs is effective for the commercial production of S. grandiflora.

Keywords

Antioxidant Activity, In vitro Micropropagation, Phenolic Content, Plant Growth Regulators, Sesbania grandiflora.
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  • Shyamala Gowri, S. and Vasantha, K., Free radical scavenging and antioxidant activity of leaves from Agathi (Sesbania grandiflora L.) Pers. Am.-Eurasian J. Sci. Res., 2010, 5, 114–119.

  • Anon., The Wealth of India: A Dictionary of Raw Materials and Industrial Products, CSIR (Govt of India), New Delhi, 1972, pp. 275–299.

  • Shareef, H., Rizwani, G. H., Zia-ul-Haq, M., Ahmad, S. and Zahid, H., Tocopherol and phytosterol profile of Sesbania grandiflora (Linn.) seed oil. J. Med. Plants Res., 2012, 6, 3478–3481.

  • Sheakh, A. A., Sayyed, Z., Siddiqui, A. R., Pratapwar, A. S. and Sheakh, S. S., Wound healing activity of Sesbania grandiflora Linn. flower ethanolic extract using excision and incision wound model in wistar rats. Int. J. PharmTech Res., 2011, 3, 895–898.

  • Arjun, P., Investigations on microprogation and isolation of active compounds from Tribulus terrestris Linn. Ph D thesis, Faculty of Sciences, University of Madras, Chennai, 2011.

  • Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant., 1962, 15, 473–497.

  • Malaisamy, M. and Mohan, N., Impact of ozone on morphological, physiological, and biochemical changes in cow pea (Vigna unguiculata [L.] Walp.). Ozone: Sci. Eng., 2014, 36, 36–42.

  • Arjun, P., Samwal, D. K., Samwal, R. B., Anita Blessy Vijayan and Krishnamoorthy, M., Quality retention and shelf-life improvement of fresh-cut apple, papaya, carrot and cucumber by chitosan-soy based edible coating. Curr. Nutr. Food Sci., 2015, 11, 282–291.

  • Kim, D. O., Jeong, S. W. and Lee, C. Y., Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem., 2003, 81, 321–326.

  • Xu, B. J. and Chang, S. K. C., A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J. Food Sci., 2007, 72, 159–166.

  • Nagarajan, S., Arjun, P., Raaman, N., Anup, S., Elizabeth Sobhia, M. and Mohan Das, T., Selective synthesis of sugar-based β-lactum derivatives: docking studies and its biological evaluation. Tetrahydron, 2012, 68, 3037–3045.

  • Asghari, G. and Lockwood, B. G., Stereospecific biotransformation of phenylethyl propionate by cell cultures of Peganum harmala L. Iran. Biomed. J., 2002, 6, 43–46.

  • Mohan, J., Kumar, V. V., Aparna, V. and Vaidya, R. P., Somatic embryogenesis and plant regeneration in Tribulus terrestris L. Phytomorphology, 2000, 50, 307–311.

  • Gurel, E. and Gurel, S., Plant regeneration from unfertilized ovaries of sugar beet (Beta vulgaris L.) cultured in vitro. Traditional J. Bot., 1998, 22, 233–238.

  • Sharma, P. and Rajam, Genotype, explant and position effects on organogenesis and somatic embryogenesis in egg plant (Solanum melongena L.). J. Exp. Bot., 1995, 46, 135–141.

  • Pande, D., Malik, S., Bora, M. and Srivastava, P. S., Rapid protocol for in vitro micropropagation of Lepidium sativum Linn. and enhancement in the yield of lepidine. In Vitro Cell. Dev. Biol., 2002, 38, 451–455.

  • Szabados, L. and Savoure, A., Proline: a multifunctional amino acid. Trends Plant Sci., 2009, 15, 89–97.

  • Uttara, B., Singh, A. V., Zamboni, P. and Mahajan, R., Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr. Neuropharmacol., 2009, 7, 65–74.

  • Harborne, J. B. and Williams, C. A., Advances in flavonoid research since 1992. Phytochemistry, 2000, 55, 481–504.

  • Benzie, I. F. and Strain, J. J., The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power the FRAP assay. Anal. Biochem., 1996, 239, 70–76.

  • Arjun, P., Saranya Sivan, P. S., Mohana Priya, S., Krishnamoorthy, M. and Balasubramanian, K., Antioxidant and antimicrobial activity of Nelumbo nucifera Gaertn. leaf extracts. J. Acad. Indust. Res., 2012, 1, 15–18.

  • Muthu Kumar, T., Mary Violet Christy, A., Mangadu, A., Malaisamy, M., Sivaraj, C., Arjun, P. and Raaman, N., Antioxidant and anticancer activity of Helicteres isora dried fruit solvent extracts. J. Acad. Indust. Res., 2012, 1, 148–152.

  • Hariprasath, L., Jegadeesh, R., Arjun, P. and Raaman, N., In vitro propagation of Senecio candicans DC and comparative antioxidant properties of aqueous extracts of the in vivo plant and in vitro derived callus. S. Afr. J. Bot., 2015, 98, 134–141.

  • Balasundram, N., Sundram, K. and Samman, S., Phenolic compounds in plant and agri-industrial byproducts: antioxidant activity, occurrence, and potential uses. Food Chem., 2006, 99, 191–203.

  • Duh, P. D., Tu, Y. Y. and Yen, G. C., Antioxidant activity of aqueous extract of Hamjyur (Chrsanthemum morifolium Ramat). Lebensm. Wiss. Technol., 1999, 32, 269–277.


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  • In vitro Micropropagation, Total Phenolic Content and Comparative Antioxidant Activity of Different Extracts of Sesbania grandiflora (L.) Pers.

Abstract Views: 429  |  PDF Views: 148

Authors

Krishnamoorthy Vinothini
Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
Masilamani Sri Devi
Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
Veronica Shalini
Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, India
Sudharshan Sekar
Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, P.O. Box 524, Auckland Park-2006, South Africa
Ruchi Badoni Semwal
Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, South Africa
Pandian Arjun
Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, South Africa
Deepak Kumar Semwal
Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, India

Abstract


Sesbania grandiflora (L.) Pers. is a common traditional medicinal plant used in bronchitis, anaemia,headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism. The present study aimed to assess plant regeneration and plantlets development in vitro using explants of S. grandiflora together with the estimation of total phenolic contentand antioxidative activity of various extracts obtained from the plant. Murashige and Skoog (MS) basal medium added with different concentrations of plant growth regulators (PGRs) was used for plant tissue culture, whereas ferric reducing antioxidant power(FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)assays were used to evaluate the antioxidant potential of different extracts of the plant. In the presence of 6-benzylaminopurine (BAP; 0.1 mg/l), the highest level(85.41%) of seed germination was achieved while the highest callus formation (96.6%) was recorded with 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg/l). Inaddition, the highest shoot induction, shoot formation and shoot elongation were observed with BAP(0.1 mg/l), indole-3-butyric acid (3 mg/l) and naphthaleacetic acid + BAP (0.4 + 0.2 mg/l) respectively.The extract of dried calluses showed highest contents of proline (110.94 mg/g), phenol (16.42 mg/g) and flavonoid (22.22 mg/g), and also highest antioxidant potential with FRAP and DPPH assays. From the present study, we may conclude that the MS basal medium supplemented with PGRs is effective for the commercial production of S. grandiflora.

Keywords


Antioxidant Activity, In vitro Micropropagation, Phenolic Content, Plant Growth Regulators, Sesbania grandiflora.

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DOI: https://doi.org/10.18520/cs%2Fv113%2Fi06%2F1142-1147