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Isolation of Listeria monocytogenes from Peridomestic Birds and Captive Wild Animals


Affiliations
1 Department of Veterinary Public Health, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
2 Department of Veterinary Pathology, Nagpur Veterinary College, Seminary Hills, Nagpur 440 006, India
3 Department of Veterinary Microbiology, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
4 Department of Veterinary Biochemistry, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
5 Rajiv Gandhi Zoological Park and Wildlife Research Center, Katraj, Pune 411 037, India
6 ICAR-National Institute of Biotic Stress Management, Raipur 493 225, India
 

Listeria monocytogenes is an important foodborne pathogen responsible for septicaemia, meningitis and abortions. There are several animal reservoirs; however, the role of wild animals and peridomestic birds remains underestimated. We have screened 270 faecal samples of wild animals in captivity (18 species) and peridomestic birds (12 species). Listeria species were isolated from seven (6.66%) mammals and two (1.21%) birds. L. monocytogenes was isolated from barking deer, porcupine, pigeon and crow. Isolated L. monocytogenes were virulent strains of 4b serogroup. There is a need to explore the role of such nonconventional sources in the spread of L. monocytogenes in nature.

Keywords

Antibiotic Sensitivity, Birds, Listeria monocytogenes, Serotyping, Wild Animals.
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  • Szymczak, B., Szymczak, M., Sawicki, W. and Dabrowski, W., Anthropogenic impact on the presence of L. monocytogenes in soil, fruits and vegetables. Folia Microbiol., 2014, 59, 23–29.

  • Rawool, D. B., Malik, S. V. S., Barbuddhe, S. B., Shakuntala, I. and Aurora, R. A., Multiplex PCR for detection of virulence associated genes in L. monocytogenes. Int. J. Food Saf., 2007, 9, 56–62.

  • Vivant, A. L., Garmyn, D. and Piveteau, P., Listeria monocytogenes, a down-to-earth pathogen. Front. Cell. Infect. Microbiol., 2013, 3, 1–10.

  • Yoshida, T., Sugimoto, T., Sato, M. and Hirai, K., Incidence of Listeria monocytogenes in wild animals in Japan. J. Vet. Med. Sci., 2000, 62, 673–675.

  • Kalorey, D. R., Kurkure, N. V., Warke, S. R., Rawool, D. B., Malik, S. V. S. and Barbuddhe, S. B., Isolation of pathogenic Listeria monocytogenes in faeces of wild animals in captivity. Comp. Immunol. Microbiol. Infect. Dis., 2006, 29, 295–300.

  • Hellstrom, S., Kiviniemi, K., Autio, T. and Korkeala, H., Listeria monocytogenes is common in wild birds in Helsinki region and genotypes are frequently similar with those found along the food chain. J. Appl. Microbiol., 2008, 104, 883–888.

  • Yadav, M. M., Roy, A., Bhanderi, B. and Jani, R. G., Prevalence of Listeria species including L. monocytogenes from apparently healthy animals at Baroda Zoo, Gujarat State, India. J. Threat. Taxa, 2011, 3, 1929–1935.

  • Saunders, D. B., Overdevest, J., Fortes, E., Windham, K., Schukken, Y., Lembo, A. and Wiedmanna, M., Diversity of Listeria species in urban and natural environments. Appl. Environ. Microbiol., 2012, 78, 4420–4433.

  • Morse, S. S., Factors in the emergence of infectious diseases. Emerg. Infect. Dis., 1995, 1, 7–15.

  • Barbuddhe, S. B., Malik, S. V. S., Kumar, J. A., Kalorey, D. R. and Chakraborty, T., Epidemiology and risk management of listeriosis in India. Int. J. Food Microbiol., 2012, 154, 113–118.

  • Doijad, S. et al., Incidence and genetic variability of Listeria species from three milk processing plants. Food Control, 2011, 22, 1900–1904.

  • Anon., ISO 11290 Part 1:1997 – horizontal method for the detection and enumeration of Listeria monocytogenes – Part 1: Detection, International Organization for Standardization, Geneva, 1997.

  • Rawool, D. B. et al., A multiplex PCR for detection of Listeria monocytogenes and its lineages. J. Microbiol. Methods, 2016, 130, 144–147.

  • Doumith, M., Buchrieser, C., Glaser, P., Jacquet, C. and Martin, P., Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J. Clin. Microbiol., 2004, 42, 3819–3822.

  • Notermans, S. H. W., Dufrenne, J., Leimeister-Wachter, M., Domann, E. and Chakraborty, T., Phosphatidylinositol specific phospholipase C activity as a marker to distinguish between pathogenic and non-pathogenic Listeria species. Appl. Environ. Microbiol., 1991, 57, 2666–2670.

  • Paziak-Domanska, B. et al., Evaluation of the API test, phosphati-dylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. Fed. Eur. Microbiol. Soc. Microbiol. Lett., 1999, 171, 209–214.

  • Suarez, M. and Vazquez-Boland, J. A., The bacterial actin nucleter protein ActA is involved in epithelial cell invasion by Listeria monocytogenes. PUBMED, 2001 (accession no. AF103807).

  • Furrer, B., Candrian, U., Hoefelein, C. and Luethy, J., Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in-vitro amplification of haemolysin gene fragments. J. Appl. Bacteriol., 1991, 70, 372–379.

  • Bauer, A. W., Kirby, W. M. M., Sherris, J. C. and Turck, M., Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Pathol., 1966, 45, 493–496.

  • Lanz, R., Kuhnert, P. and Boerlin, P., Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland. Vet. Microbiol., 2003, 91, 73–84.

  • Chasseignaux, E., Gerault, P., Toquin, M. T., Salvat, G., Colin, P. and Ermel, G., Ecology of Listeria monocytogenes in the environment of raw poultry meat and raw pork meat processing plants. FEMS Microbiol. Lett., 2002, 210, 271–275.

  • Ho, A. J., Lappi, V. R. and Wiedmann, M., Longitudinal monitoring of Listeria monocytogenes contamination patterns in a farmstead dairy processing facility. J. Dairy Sci., 2007, 90, 2517–2524.

  • Arumugaswamy, R. and Gibson, I., Listeria in zoo animals and rivers. Aust. Vet. J., 1999, 77, 819–820.

  • Bauwens, L., Vercammmen, F. and Hertsens, A., Detection of pathogenic Listeria spp. in zoo animal faeces: use of chromogenic separation and a chromogenic isolation medium. Vet. Microbiol., 2003, 91, 115–123.

  • Fenlon, D. R., Wild birds and silage as reservoirs of Listeria in the agricultural environment. J. Appl. Bacteriol., 1985, 59, 537–543.

  • Gnat, S. et al., Experimental studies of microbial populations and incidence of zoonotic pathogens in the faeces of red deer (Cervus elaphus). Lett. Appl. Microbiol., 2015, 61, 446–452.

  • Sarangi, L. N. and Panda, H. K., Occurrence of Listeria species in different captive wild animals of Nandankanan Zoo, Baranga, Odisha, India. J. Threat. Taxa, 2013, 5, 3542–3547.

  • Barbuddhe, S. B. et al., Presence of a widely disseminated Listeria monocytogenes serotype 4b clone in India. Emerg. Microbes Infect., 2016, 5, e55.


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  • Isolation of Listeria monocytogenes from Peridomestic Birds and Captive Wild Animals

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Authors

U. V. Jagtap
Department of Veterinary Public Health, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
R. P. Kolhe
Department of Veterinary Public Health, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
P. D. Deshpande
Department of Veterinary Public Health, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
N. V. Kurkure
Department of Veterinary Pathology, Nagpur Veterinary College, Seminary Hills, Nagpur 440 006, India
C. V. Dhandore
Department of Veterinary Public Health, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
D. M. Muglikar
Department of Veterinary Microbiology, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
S. N. Jadhav
Department of Veterinary Biochemistry, Krantisinh Nana Patil College of Veterinary Sciences, Shirwal 412 801, India
N. K. Nighot
Rajiv Gandhi Zoological Park and Wildlife Research Center, Katraj, Pune 411 037, India
S. B. Barbuddhe
ICAR-National Institute of Biotic Stress Management, Raipur 493 225, India

Abstract


Listeria monocytogenes is an important foodborne pathogen responsible for septicaemia, meningitis and abortions. There are several animal reservoirs; however, the role of wild animals and peridomestic birds remains underestimated. We have screened 270 faecal samples of wild animals in captivity (18 species) and peridomestic birds (12 species). Listeria species were isolated from seven (6.66%) mammals and two (1.21%) birds. L. monocytogenes was isolated from barking deer, porcupine, pigeon and crow. Isolated L. monocytogenes were virulent strains of 4b serogroup. There is a need to explore the role of such nonconventional sources in the spread of L. monocytogenes in nature.

Keywords


Antibiotic Sensitivity, Birds, Listeria monocytogenes, Serotyping, Wild Animals.

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DOI: https://doi.org/10.18520/cs%2Fv113%2Fi09%2F1783-1787