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Phytoecdysteroid Profiling of Silene vulgaris by UPLC-ESI-MS


Affiliations
1 Natural Products Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, India
 

Silene vulgaris is a wild edible plant consumed in both raw as well as cooked forms in several parts of Europe. The phytoconstituents of Silene species include phytoecdysteroids, triterpenoidal saponins, terpenoids, flavonoids and phenolics. Silene vulgaris is a relatively unexplored species and the chemical profiling of this plant has not been attempted so far. Hence the UPLC-ESI-MS approach was applied to the extracts of flowers, leaves and ischolar_mains of S. vulgaris for the profiling of phytoecdysteroids. The relative distribution of these compounds varied between flowers and leaves; however, the qualitative composition was similar. Only traces of phytoecdysteroids were present in the ischolar_mains. The aglycones, sugars and other moieties were determined on the basis of ESI-MS. A total of eight previously known phytoecdysteroids were identified. Partial characterization of eight other phytoecdysteroids was also attempted.

Keywords

Chemical Profiling, Chromatographic Analysis, Phytoecdysteroids, Silene vulgaris.
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  • Phytoecdysteroid Profiling of Silene vulgaris by UPLC-ESI-MS

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Authors

Jasmeen Sidana
Natural Products Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, India
Renuka Devi
Natural Products Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, India
Pawan Kumar
Natural Products Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, India
Bikram Singh
Natural Products Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, India
Om P. Sharma
Natural Products Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, India

Abstract


Silene vulgaris is a wild edible plant consumed in both raw as well as cooked forms in several parts of Europe. The phytoconstituents of Silene species include phytoecdysteroids, triterpenoidal saponins, terpenoids, flavonoids and phenolics. Silene vulgaris is a relatively unexplored species and the chemical profiling of this plant has not been attempted so far. Hence the UPLC-ESI-MS approach was applied to the extracts of flowers, leaves and ischolar_mains of S. vulgaris for the profiling of phytoecdysteroids. The relative distribution of these compounds varied between flowers and leaves; however, the qualitative composition was similar. Only traces of phytoecdysteroids were present in the ischolar_mains. The aglycones, sugars and other moieties were determined on the basis of ESI-MS. A total of eight previously known phytoecdysteroids were identified. Partial characterization of eight other phytoecdysteroids was also attempted.

Keywords


Chemical Profiling, Chromatographic Analysis, Phytoecdysteroids, Silene vulgaris.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi10%2F1986-1992