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IHHNV Infection from the Wild Shrimps of Andaman and Nicobar Islands, India


Affiliations
1 ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
2 ICAR-Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, Raja Annamalai Puram, Chennai 600 028, India
 

The present study was intended to screen the wild shrimps of Andaman and Nicobar Islands (ANI) against infectious diseases. A total of 175 shrimp samples (35 pools) consisting of Fenneropenaeus indicus, Penaeus monodon, Penaeus merguiensis and Metapenaeus monoceros were collected from different landing centres across ANI. Out of 35 pools of samples analysed by polymerase chain reaction (PCR), a total of 10 pools of Penaeus monodon collected from Betapur (1 pool), Lohabarrack (4 pools) and Campbell Bay (5 pools) were found positive for Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV). Nucleotide sequence of IHHNV isolated from ANI showed 100% identity to the sequences of IHHNV reported from Vietnam, Taiwan, Australia, China, Egypt, USA, Ecuador, 99% identity to IHHNV reported from Brazil, Venezuela, Korea, 96% identity to IHHNV reported from Thailand and 95% identity to IHHNV reported from India. Based on phylogenetic tree analysis, IHHNV of ANI is closely related to IHHNV of Vietnam. Histopathological analysis revealed typical eosinophilic intranuclear cowdry type A inclusion bodies in gill lamellae which further confirmed the IHHNV infection. The present study provides a definitive evidence for the first report of infectious IHHNV in wild P. monodon from ANI.

Keywords

Andaman and Nicobar Islands, Disease Surveillance, IHHNV, Penaeus monodon, Wild Shrimp.
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  • IHHNV Infection from the Wild Shrimps of Andaman and Nicobar Islands, India

Abstract Views: 321  |  PDF Views: 127

Authors

K. Saravanan
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
P. Puneeth Kumar
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
Arunjyoti Baruah
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
J. Praveenraj
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
T. Sathish Kumar
ICAR-Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, Raja Annamalai Puram, Chennai 600 028, India
S. Pramod Kumar
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
T. Sivaramakrishnan
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
A. Anuraj
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
J. Raymond Jani Angel
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
R. Kiruba Sankar
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India
S. Dam Roy
ICAR-Central Island Agricultural Research Institute, Port Blair 744 105, India

Abstract


The present study was intended to screen the wild shrimps of Andaman and Nicobar Islands (ANI) against infectious diseases. A total of 175 shrimp samples (35 pools) consisting of Fenneropenaeus indicus, Penaeus monodon, Penaeus merguiensis and Metapenaeus monoceros were collected from different landing centres across ANI. Out of 35 pools of samples analysed by polymerase chain reaction (PCR), a total of 10 pools of Penaeus monodon collected from Betapur (1 pool), Lohabarrack (4 pools) and Campbell Bay (5 pools) were found positive for Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV). Nucleotide sequence of IHHNV isolated from ANI showed 100% identity to the sequences of IHHNV reported from Vietnam, Taiwan, Australia, China, Egypt, USA, Ecuador, 99% identity to IHHNV reported from Brazil, Venezuela, Korea, 96% identity to IHHNV reported from Thailand and 95% identity to IHHNV reported from India. Based on phylogenetic tree analysis, IHHNV of ANI is closely related to IHHNV of Vietnam. Histopathological analysis revealed typical eosinophilic intranuclear cowdry type A inclusion bodies in gill lamellae which further confirmed the IHHNV infection. The present study provides a definitive evidence for the first report of infectious IHHNV in wild P. monodon from ANI.

Keywords


Andaman and Nicobar Islands, Disease Surveillance, IHHNV, Penaeus monodon, Wild Shrimp.

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DOI: https://doi.org/10.18520/cs%2Fv113%2Fi10%2F2027-2031