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Detection of IHHN Virus in a Specific Pathogen Free Bonafide Penaeus vannamei (Boone, 1931) Broodstock Consignment Imported to India


Affiliations
1 Rajiv Gandhi Centre for Aquaculture, MPEDA (Ministry of Commerce and Industry, Government of India), TNFDC Hatchery Complex, Kapaleeswarar Nagar, Neelankarai, Chennai 600 041, India
2 National Institute of Ocean Technology, Palli Karanai, Chennai 600 100, India
3 Rajiv Gandhi Centre for Aquaculture, Technology Transfer Training and Administrative Complex, MPEDA (Ministry of Commerce and Industry, Govt of India), 3/197, Poompuhar Road, Karaimedu, Sattanathapuram 609 109, India
 

The World Organization for Animal Health (formerly Office International des Epizooties OIE) listed pathogenic shrimp virus, infectious hypodermal haematopoetic necrosis virus (IHHNV), has been detected in a specific pathogen free (SPF) declared consignment imported from an approved SPF vannamei broodstock supplier to India. The Aquatic Quarantine Facility (AQF), Chennai the only Government-approved quarantine premise for ensuring the SPF status of imported Penaeus vannamei broodstock detected this pathogen during its routine screening process. The AQF, which has so far screened about 1068 SPF batches of vannamei consignment, recorded that one particular batch imported to India was not SPF, though the exporting country declared the stock as SPF. The presence of the viral pathogen was confirmed by both nested PCR and quantitative PCR (qPCR). Sequencing data of the target viral genome revealed 100% similarity with the already published decapod penstyldensovirus-1 isolate IHHNV partial genome (GenBank accession no. KJ862253). The test results declared by AQF were further validated by the Central Institute of Brackishwater Aquaculture, Chennai, which is the referral laboratory of AQF. As the import of non-SPF stock into the country is a source of biosecurity threat, the entire batch was incinerated according to the standard operating procedure of AQF. Thus the stringent biosecurity measures and disease diagnostic strategies followed at AQF served to safeguard the industry from this virus, which would have otherwise gained entry into the country through the falsely claimed SPF stock. This communication highlights the importance of SPF stock and detection of IHHNV.

Keywords

Bonafide, Shrimp Aquaculture, Broodstock, Infectious Hypodermal Haematopoetic Necrosis Virus, Specific Pathogen Free.
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  • Remany, M. C., Cyriac, D., Nagaraj, S., Rao, B., Panda, A. K., Kumar, J. and Thampi Samraj, Y. C., Specific pathogen free assurance of imported Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in the aquatic quarantine facility, Chennai. Curr. Sci., 2010, 99, 1656–1658.

  • Press Information Bureau, Ministry of Commerce and Industry, Government of India, dated 3 July 2015; http://commerce.nic.in/MOC/press_release.asp?id=3215.

  • FAO, State of world aquaculture. FAO Fisheries Technical Paper 500, Food and Agriculture Organization of the United Nations, Rome, 2006, p. 134.

  • List of approved suppliers for import of SPF broodstock of L. vannamei; http://www.caa.gov.in/broodstock_suppliers.html

  • Department of Animal Husbandry, Dairying and Fisheries order No. 35029/13/2008-Fy (T&E), Standard operating procedures for Aquatic Quarantine Facility for L. vannamei, 2 June 2009.

  • Lightner, D. V., Biosecurity in Aquaculture Production Systems: Exclusion of Pathogens and other Undesirables, The World Aquaculture Society, Batton Rouge, Louisiana, USA, 2003, pp. 81–116.

  • Arthur, J. R., Bondad-Reantoso, M. G. and Subasinghe, R. P., Procedures for the quarantine of live aquatic animals – a manual, FAO Fisheries Tech. Paper No. 502, 2008.

  • Lightner, D. V., Redman, R. M., Arce, S. and Moss S. M., Specific pathogen free (SPF) shrimp stocks in shrimp farming facilities as a novel method for disease control in crustaceans. In Shellfish Safety and Quality (eds Shumway, S. and Rodrick, G.), Woodhead Publishers, London, 2009, pp. 384–424.

  • Lightner, D. V. and Redman, R. M., Shrimp diseases and current diagnostic methods. Aquaculture, 1998, 164, 201–220.

  • IQ 2000 IHHNV (nested). Detection and prevention system. 2015/09 Manual; http://www.iq2000kit.com

  • Remany, M. C. et al., Effect of preservatives, temperature and storage duration on stability of nucleic acids of pleopod tissues of P. vannamei and screening of viral infections. Indian J. Exp. Biol., 2015, 53, 665–670.

  • IQREALTM IHHNV Quantitative System.2015/02 Manual; http://www.iq2000kit.com

  • Heid, C. A., Stevens, L., Livak, K. J. and Williams, P. M., Real time quantitative PCR. Genome Res., 1996, 6, 986–994.

  • Tang, K. F. J. and Lightner, D. V., Detection and quantification of IHHNV in penaeid shrimp by real time PCR. Dis. Aquat. Org., 2001, 44, 79–85.

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S., Mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 2011, 28, 2731–2739.

  • Silvaa, D. C. D., Nunes, A. R. D., Teixeirac, D. I. A., Lima, J. P. M. S. and Lanzaa, D. C. F., Infectious hypodermal and hematopoietic necrosis virus from Brazil: sequencing, comparative analysis and PCR detection. Virus. Res., 2014, 189, 136–146.

  • Lightner, D. V. and Redman, R. M., Infectious hypodermal and haematopoetic necrosis, a newly recognized viral disease of penaeid shrimp. J. Invertebr. Pathol., 1983, 142, 62–70.

  • Tang, K. F. J. and Lightner, D. V., Low sequence variation among isolates of infectious hypodermal and hematopoietic necrosis virus (IHHNV) originating from Hawaii and the Americas. Dis. Aquat. Org., 2002, 49, 93–97.

  • Bonami, J. R., Trumper, B., Mari, J., Brehelin, M. and Lightner, D. V., Purification and characterization of the infectious hypodermal and hematopoietic necrosis virus of penaeid shrimps. J. Gen. Virol., 1990, 71, 2657–2664.

  • OIE, In Manual of Diagnostic Tests for Aquatic Animal Diseases, Infectious hypodermal and hematopoietic necrosis, Office International des Epizooties, Paris, France, 2009, 6th edn, p. 467.

  • Tang, K. F. J. and Lightner, D. V., Infectious hypodermal and hematopoietic necrosis virus (IHHNV) in the genome of the black tiger prawn Penaeus monodon from Africa and Australia. Virus Res., 2006, 118, 185–191.

  • Primavera, J. H. and Quinitio, E. T., Runt-deformity syndrome in cultured giant tiger prawn Penaeus monodon. J. Crustacean Biol., 2000, 20, 796–802.

  • Gunalan, B., Soundarapandian, P., Anand, T., Kotiya Anil, S. and Simon, N. T., Disease occurrence in Litopenaeus vannamei shrimp culture systems in different geographical regions of India. Int. J. Aquacult., 2014, 4(4), 24–28.

  • Motte, E. et al., Prevention of IHHNV vertical transmission in the white shrimp Penaeus vannamei. Aquaculture, 2003, 219, 57–70.

  • Tang, K. F. J., Poulos, B. T., Wang, J., Redman, R. M., Hsiu-HuiShih and Lightner, D. V., Geographic variations among IHHNV isolates and characteristics of their infection. Dis. Aquat. Org., 2003, 53, 91–99.

  • Tang, K. F. J., Validation of IQ 2000TM nested IHHNV PCR detection and prevention system developed by Farming Intelligene Technology Corp. Validation certificate issued by the University of Arizona to M/s Genereach Biotechnology Corp, 14 September 2008.


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  • Detection of IHHN Virus in a Specific Pathogen Free Bonafide Penaeus vannamei (Boone, 1931) Broodstock Consignment Imported to India

Abstract Views: 450  |  PDF Views: 156

Authors

M. C. Remany
Rajiv Gandhi Centre for Aquaculture, MPEDA (Ministry of Commerce and Industry, Government of India), TNFDC Hatchery Complex, Kapaleeswarar Nagar, Neelankarai, Chennai 600 041, India
R. Kirubagaran
National Institute of Ocean Technology, Palli Karanai, Chennai 600 100, India
Daly Cyriac
Rajiv Gandhi Centre for Aquaculture, MPEDA (Ministry of Commerce and Industry, Government of India), TNFDC Hatchery Complex, Kapaleeswarar Nagar, Neelankarai, Chennai 600 041, India
O. C. Sruthi Prem
Rajiv Gandhi Centre for Aquaculture, MPEDA (Ministry of Commerce and Industry, Government of India), TNFDC Hatchery Complex, Kapaleeswarar Nagar, Neelankarai, Chennai 600 041, India
P. Krishnakanth Varadaraju
Rajiv Gandhi Centre for Aquaculture, MPEDA (Ministry of Commerce and Industry, Government of India), TNFDC Hatchery Complex, Kapaleeswarar Nagar, Neelankarai, Chennai 600 041, India
A. K. Panda
Rajiv Gandhi Centre for Aquaculture, MPEDA (Ministry of Commerce and Industry, Government of India), TNFDC Hatchery Complex, Kapaleeswarar Nagar, Neelankarai, Chennai 600 041, India
Jaideep Kumar
Rajiv Gandhi Centre for Aquaculture, Technology Transfer Training and Administrative Complex, MPEDA (Ministry of Commerce and Industry, Govt of India), 3/197, Poompuhar Road, Karaimedu, Sattanathapuram 609 109, India
Y. C. Thampi Samraj
Rajiv Gandhi Centre for Aquaculture, Technology Transfer Training and Administrative Complex, MPEDA (Ministry of Commerce and Industry, Govt of India), 3/197, Poompuhar Road, Karaimedu, Sattanathapuram 609 109, India

Abstract


The World Organization for Animal Health (formerly Office International des Epizooties OIE) listed pathogenic shrimp virus, infectious hypodermal haematopoetic necrosis virus (IHHNV), has been detected in a specific pathogen free (SPF) declared consignment imported from an approved SPF vannamei broodstock supplier to India. The Aquatic Quarantine Facility (AQF), Chennai the only Government-approved quarantine premise for ensuring the SPF status of imported Penaeus vannamei broodstock detected this pathogen during its routine screening process. The AQF, which has so far screened about 1068 SPF batches of vannamei consignment, recorded that one particular batch imported to India was not SPF, though the exporting country declared the stock as SPF. The presence of the viral pathogen was confirmed by both nested PCR and quantitative PCR (qPCR). Sequencing data of the target viral genome revealed 100% similarity with the already published decapod penstyldensovirus-1 isolate IHHNV partial genome (GenBank accession no. KJ862253). The test results declared by AQF were further validated by the Central Institute of Brackishwater Aquaculture, Chennai, which is the referral laboratory of AQF. As the import of non-SPF stock into the country is a source of biosecurity threat, the entire batch was incinerated according to the standard operating procedure of AQF. Thus the stringent biosecurity measures and disease diagnostic strategies followed at AQF served to safeguard the industry from this virus, which would have otherwise gained entry into the country through the falsely claimed SPF stock. This communication highlights the importance of SPF stock and detection of IHHNV.

Keywords


Bonafide, Shrimp Aquaculture, Broodstock, Infectious Hypodermal Haematopoetic Necrosis Virus, Specific Pathogen Free.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi01%2F196-200