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Nanocellulose as Functional Filler in Starch/Polyvinyl Alcohol Film for Preparation of Urea Biosensor


Affiliations
1 ICAR-Central Institute for Research on Cotton Technology, Matunga, Mumbai 400 019, India
 

Urea analysis is of considerable interest in clinical, agricultural and environmental chemistry. Urea biosensor helps in on-site monitoring of urea. The aim of this study was to evaluate the potential use of nanocellulose reinforced composite film as substrate for urea biosensor. Nanocellulose was prepared from non-spinnable short staple cotton fibres by controlled microbial hydrolysis process. This was used as a substrate to immobilize urease enzyme and, subsequently used to reinforce the starch/polyvinyl alcohol biocomposite film. Nanocellulose acted as both carrier for the enzyme and reinforcing agent in the film. Use of 1.5% nanocellulose increased the tensile strength of the resultant film to the tune of 2.5-fold. About 68% immobilization efficiency of urease onto nanocellulose was observed in the reported process. The biosensor could detect the presence of urea linearly in the range of 10 to 1000 ppm concentration in water with a response time between 30 sec and 1 min. The storage lifetime was 2 months when stored in 4°C with the activity more than 90%. The study revealed that the nanocomposite film could significantly improve the performance and storability of urea biosensor.

Keywords

Functional Filler, Nanocellulose, Starch Film, Urea Biosensor, Urease Immobilization.
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  • Turner, A. P. F., Biosensors: Sense and sensibility. Chem. Soc. Rev., 2013, 42, 3184–3196.

  • Ispas, C. R., Crivat, G. and Andreescu, S., Review: recent developments in enzyme-based biosensors for biomedical analysis. Anal. Lett., 2012, 45, 168–186.

  • Liu, Y. and Chen, J. Y., Enzyme immobilization on cellulose matrixes. J. Bioact. Compat. Polym., 2016, 31, 553–567.

  • Kotel’nikova, N. E., Mikhailova, S. A. and Vlasova, E. N., Immobilization of proteolytic enzymes trypsin and alpha-chymotrypsin to cellulose matrix. Russ. J. Appl. Chem., 2007, 80, 322–329.

  • Liu, Y., Matharu, Z., Howland, M. C., Revzin, A. and Simonian, A. L., Affinity and enzyme-based biosensors: Recent advances and emerging applications in cell analysis and point-of-care testing. Anal. Bioanal. Chem., 2012, 404, 1181–1196.

  • Marchenko, S. V., Kucherenko, I. S., Hereshko, A. N., Panasiuk, I. V., Soldatkin, O. O., El’skaya, A. V. and Soldatkin, A. P., Application of potentiometric biosensor based on recombinant urease for urea determination in blood serum and hemodialyzate. Sens. Actuators B Chem., 2015, 207(Part B), 981–986.

  • Incani, V., Danumah, C. and Boluk, Y., Nanocomposites of nanocrystalline cellulose for enzyme immobilization. Cellulose, 2013, 20, 191–200.

  • Monier, M. and El-Sokkary, A. M. A., Modification and characterization of cellulosic cotton fibers for efficient immobilization of urease. Int. J. Biol. Macromol., 2012, 51, 18–24.

  • Akduman, B., Uygun, M., Çoban, E. P., Uygun, D. A., Bıyık, H. and Akgol, S., Reversible immobilization of urease by using bacterial cellulose nanofibers. Appl. Biochem. Biotechnol., 2013, 171, 2285–2294.

  • Park, H.-R., Chough, S.-H., Yun, Y.-H. and Yoon, S.-D., Properties of starch/pva blend films containing citric acid as additive. J. Polym. Environ., 2005, 13, 375–382.

  • Satyamurthy, P., Jain, P., Balasubramanya, R. H. and Vigneshwaran, N., Preparation and characterization of cellulose nanowhiskers from cotton fibres by controlled microbial hydrolysis. Carbohydr. Polym., 2011, 83, 122–129.

  • Yang, Q. and Pan, X., A facile approach for fabricating fluorescent cellulose. J. Appl. Polym. Sci., 2010, 117, 3639–3644.

  • Selvamurugan, C., Lavanya, A. and Sivasankar, B., A comparative study on immobilization of urease on different matrices. J. Sci. Ind. Res., 2007, 66, 655–659.

  • Fawcett, J. K. and Scott, J. E., A rapid and precise method for the determination of urea. J. Clin. Pathol., 1960, 13, 156–159.

  • Savadekar, N., Karande, V., Vigneshwaran, N., Kadam, P. and Mhaske, S., Preparation of cotton linter nanowhiskers by highpressure homogenization process and its application in thermoplastic starch. Appl. Nanosci., 2015, 5, 281–290.

  • Lee, S.-Y., Mohan, D. J., Kang, I.-A., Doh, G.-H., Lee, S. and Han, S. O., Nanocellulose reinforced pva composite films: effects of acid treatment and filler loading. Fibers Polym., 2009, 10, 77–82.

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., Protein measurement with the folin phenol reagent. J. Biol. Chem., 1951, 193, 265–275.

  • Dindar, B., Karakuş, E. and Abasıyanık, F., New urea biosensor based on urease enzyme obtained from helycobacter pylori. Appl. Biochem. Biotechnol., 2011, 165, 1308–1321.


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  • Nanocellulose as Functional Filler in Starch/Polyvinyl Alcohol Film for Preparation of Urea Biosensor

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Authors

Prasad Satyamurthy
ICAR-Central Institute for Research on Cotton Technology, Matunga, Mumbai 400 019, India
Vigneshwaran Nadanathangam
ICAR-Central Institute for Research on Cotton Technology, Matunga, Mumbai 400 019, India

Abstract


Urea analysis is of considerable interest in clinical, agricultural and environmental chemistry. Urea biosensor helps in on-site monitoring of urea. The aim of this study was to evaluate the potential use of nanocellulose reinforced composite film as substrate for urea biosensor. Nanocellulose was prepared from non-spinnable short staple cotton fibres by controlled microbial hydrolysis process. This was used as a substrate to immobilize urease enzyme and, subsequently used to reinforce the starch/polyvinyl alcohol biocomposite film. Nanocellulose acted as both carrier for the enzyme and reinforcing agent in the film. Use of 1.5% nanocellulose increased the tensile strength of the resultant film to the tune of 2.5-fold. About 68% immobilization efficiency of urease onto nanocellulose was observed in the reported process. The biosensor could detect the presence of urea linearly in the range of 10 to 1000 ppm concentration in water with a response time between 30 sec and 1 min. The storage lifetime was 2 months when stored in 4°C with the activity more than 90%. The study revealed that the nanocomposite film could significantly improve the performance and storability of urea biosensor.

Keywords


Functional Filler, Nanocellulose, Starch Film, Urea Biosensor, Urease Immobilization.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi04%2F897-901