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Enhanced Oil Accumulation in Tobacco (Nicotiana tabacum L.) Leaves by Ectopic Overexpression of VgDGAT1a for Renewable Production of Biofuels


Affiliations
1 Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
2 Department of Life Science, Heze University, Heze, Shandong 274015, China
 

To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5–5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.

Keywords

Biofuels, Metabolic Engineering, Plant Oil, Tobacco Leaves, VgDGAT1a Gene.
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  • Enhanced Oil Accumulation in Tobacco (Nicotiana tabacum L.) Leaves by Ectopic Overexpression of VgDGAT1a for Renewable Production of Biofuels

Abstract Views: 449  |  PDF Views: 131

Authors

Chang-Yong Gao
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
Xue Mao
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
Hong-Qin Shang
Department of Life Science, Heze University, Heze, Shandong 274015, China
Fang Li
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
Run-Zhi Li
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China

Abstract


To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5–5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.

Keywords


Biofuels, Metabolic Engineering, Plant Oil, Tobacco Leaves, VgDGAT1a Gene.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi06%2F1234-1240