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Comparison of Culture and PCR Methods for Diagnosis of Group B Streptococcus in Women


Affiliations
1 Department of Microbiology, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of
2 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Islamic Republic of
3 Department of Microbiology, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of
4 Student Research Committee, Department of Epidemiology and Biostatistics, Mashhad University of Medical Sciences, Mashhad, Iran, Islamic Republic of
5 School of Medicine, Islamic Azad University, Sari Branch, Sari, Iran, Islamic Republic of
 

Group B Streptococcus (GBS), as a risk factor for newborns, is one of the most important causes of meningitis and septicaemia in newborns, intrauterine infections in women and colonization in the vaginal region in late gestation. We evaluate GBS prevalence and infection among pregnant women through culture and PCR methods, and then compare these methods. To this end, vaginal and urine swabs were separately collected from 246 women at the Women’s Hospital of Sari, Iran. Next, the samples were enriched in selective culture media Todd–Hewitt broth for 24 h at 37°C, recognized using blood agar media, and finally were amplified STREP gene by PCR technique. The indicated that the frequency of GBS in samples collected from urine and vaginal cultures and PCR method was positive. In addition, no significant relationship was found among the positive results of culture, maternal age, gestational age, a history of abortion and infection. With regard to the cultivation method as a standard technique, the sensitivity of PCR test was 100% and specificity was 96%. Moreover, it was found that the colonization rate of GBS in women was significant in Sari. Therefore, PCR is recommended as a reliable and rapid method for detection of GBS.

Keywords

Culture, Detection, Group B Streptococcus, Polymerase Chain Reaction, Women, Pregnancy.
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  • Comparison of Culture and PCR Methods for Diagnosis of Group B Streptococcus in Women

Abstract Views: 390  |  PDF Views: 129

Authors

Mohtaram Nasrollahi
Department of Microbiology, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of
Masoud Moghadaszadeh
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Islamic Republic of
Maryam Abdollahi
Department of Microbiology, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of
Zeynab Marzhoseyni
Department of Microbiology, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of
Maryam Salehian
Department of Microbiology, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of
Eisa Nazar
Student Research Committee, Department of Epidemiology and Biostatistics, Mashhad University of Medical Sciences, Mashhad, Iran, Islamic Republic of
Shukufe Sharif
School of Medicine, Islamic Azad University, Sari Branch, Sari, Iran, Islamic Republic of
Behnam Hashemi
Department of Microbiology, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of

Abstract


Group B Streptococcus (GBS), as a risk factor for newborns, is one of the most important causes of meningitis and septicaemia in newborns, intrauterine infections in women and colonization in the vaginal region in late gestation. We evaluate GBS prevalence and infection among pregnant women through culture and PCR methods, and then compare these methods. To this end, vaginal and urine swabs were separately collected from 246 women at the Women’s Hospital of Sari, Iran. Next, the samples were enriched in selective culture media Todd–Hewitt broth for 24 h at 37°C, recognized using blood agar media, and finally were amplified STREP gene by PCR technique. The indicated that the frequency of GBS in samples collected from urine and vaginal cultures and PCR method was positive. In addition, no significant relationship was found among the positive results of culture, maternal age, gestational age, a history of abortion and infection. With regard to the cultivation method as a standard technique, the sensitivity of PCR test was 100% and specificity was 96%. Moreover, it was found that the colonization rate of GBS in women was significant in Sari. Therefore, PCR is recommended as a reliable and rapid method for detection of GBS.

Keywords


Culture, Detection, Group B Streptococcus, Polymerase Chain Reaction, Women, Pregnancy.

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DOI: https://doi.org/10.18520/cs%2Fv114%2Fi08%2F1738-1741