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Identification of Bioactive Compound from Citrus maxima Fruit against Carbohydrate-Hydrolysing Enzymes


Affiliations
1 Department of Fruit and Vegetable Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India
2 Department of Food Protectants and Infestation Control, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India
3 Department of Spices and Flavor Science, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India
 

The aim of this study was to identify the bioactive compound of Citrus maxima fruit that targets the enzymes responsible for hyperglycaemia. Preliminary in vitro screening of extracts of fruit parts revealed that juicy segments (ethanol, methanol and aqueous) showed the highest inhibition towards the carbohydrate- hydrolysing enzymes. Further, juicy segment extracts were subjected to preparative HPLC for separation and isolation of compounds. After a series of separations, in vitro analysis and characterization, naringin was identified to be the bioactive compound that is responsible for inhibiting carbohydrate-hydrolysing enzyme activities. Further application, such as developing food formulations from C. maxima will benefit diabetic populations.

Keywords

Bioactive Compound, Citrus maxima, Carbohydrate- Hydrolysing Enzymes, Hyperglycaemia.
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  • Identification of Bioactive Compound from Citrus maxima Fruit against Carbohydrate-Hydrolysing Enzymes

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Authors

S. K. Reshmi
Department of Fruit and Vegetable Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India
H. K. Manonmani
Department of Food Protectants and Infestation Control, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India
J. R. Manjunatha
Department of Spices and Flavor Science, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India
M. N. Shashirekha
Department of Fruit and Vegetable Technology, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India

Abstract


The aim of this study was to identify the bioactive compound of Citrus maxima fruit that targets the enzymes responsible for hyperglycaemia. Preliminary in vitro screening of extracts of fruit parts revealed that juicy segments (ethanol, methanol and aqueous) showed the highest inhibition towards the carbohydrate- hydrolysing enzymes. Further, juicy segment extracts were subjected to preparative HPLC for separation and isolation of compounds. After a series of separations, in vitro analysis and characterization, naringin was identified to be the bioactive compound that is responsible for inhibiting carbohydrate-hydrolysing enzyme activities. Further application, such as developing food formulations from C. maxima will benefit diabetic populations.

Keywords


Bioactive Compound, Citrus maxima, Carbohydrate- Hydrolysing Enzymes, Hyperglycaemia.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi10%2F2099-2105