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In vitro Plant Development of Eleusine coracana via Indirect Organogenesis and Somatic Embryogenesis Using Mature Seeds as Explants


Affiliations
1 Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, India
2 Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, India
 

A complete plant regeneration system has been developed using mature seeds as explants. Mature embryos were used for induction of callus and regeneration of finger millet GE-3885 genotype. Highest callus induction and proliferation was found with MS + 1.5 mg/l NAA and MS+0.5 mg/l NAA respectively. Somatic embryos were obtained in MS + 0.5 mg/l 2,4-D. Histological analysis of somatic embryos revealed globular-shaped structure. Plantlets attained good length of shoots and ischolar_mains on MS + 1.5 mg/l BA and MS + 1.5 mg/l IBA respectively, and were acclimatized under glasshouse conditions after proliferation of ischolar_mains in hydroponics system.

Keywords

Embryogenesis, Finger Millet, Indirect Organogenesis, Mature Seeds, Somatic Millets.
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  • In vitro Plant Development of Eleusine coracana via Indirect Organogenesis and Somatic Embryogenesis Using Mature Seeds as Explants

Abstract Views: 555  |  PDF Views: 139

Authors

Anamika Kashyap
Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, India
Santosh Madanrao Penak
Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, India
Arnab Saha
Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, India
B. R. Singh
Govind Ballabh Pant University of Agriculture and Technology, Pantnagar 263 145, India

Abstract


A complete plant regeneration system has been developed using mature seeds as explants. Mature embryos were used for induction of callus and regeneration of finger millet GE-3885 genotype. Highest callus induction and proliferation was found with MS + 1.5 mg/l NAA and MS+0.5 mg/l NAA respectively. Somatic embryos were obtained in MS + 0.5 mg/l 2,4-D. Histological analysis of somatic embryos revealed globular-shaped structure. Plantlets attained good length of shoots and ischolar_mains on MS + 1.5 mg/l BA and MS + 1.5 mg/l IBA respectively, and were acclimatized under glasshouse conditions after proliferation of ischolar_mains in hydroponics system.

Keywords


Embryogenesis, Finger Millet, Indirect Organogenesis, Mature Seeds, Somatic Millets.

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DOI: https://doi.org/10.18520/cs%2Fv115%2Fi1%2F91-98