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Multiresidue Determination of Pesticides in Market Honey from Northern India Using QuEChERS Approach and Assessment of Potential Risks to Consumers


Affiliations
1 CSK HP Agricultural University, Palampur - 176 062, India
2 Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, India
 

Honey has multiple beneficial properties but polluted environments have led to its contamination. Contaminated honey not only serves as a sentinel of environmental pollution, but can also pose potential risks to consumers’ health. In the present study, QuEChERS (quick, easy, cheap, effective, rugged and safe) method along with gas chromatography coupled to selective detectors (ECD/FTD/MS) was used for determining 24 pesticide residues and/or their metabolites in 150 honey samples collected from markets in Northern India. The method was optimized and validated according to the European Commission’s guidelines. Residues of pesticides were detected in 12% of samples, of which a majority contained organophosphate residues. Assessment of human health risks suggests that contaminated honey at current levels has minimal contribution to toxicological risks. However, precautionary measures should always be taken considering the customary honey feeding in infants and cumulative effect of these chemicals in the foreseeable future. This study highlights the importance of continuous monitoring of pesticide residues, and consumer awareness towards certified products to safeguard public health.

Keywords

Honey, Northern India, Pesticides, Risk Assessment, QuEChERS.
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  • Kumar, A., Gill, J. P. S., Bedi, J. S., Kumar, A. and Chhuneja, P. K., Determination of antibiotic residues in honey by HPLC-DAD and assessment of potential risks to consumers. In Proceedings of the 15th Annual Conference of IAVPHS and National Symposium on Intersectoral Approaches to Combat Zoonoses: Strategies and Challenge, Tirupati, India, 2017, p. 266.

  • Tsipi, D., Triantafyllou, M. and Hiskia, A., Determination of organochlorine pesticide residues in honey, applying solid phase extraction with RP-C18 material. Analyst, 1999, 124(4), 473–475.

  • Christodoulou, D. L., Kanari, P., Kourouzidou, O., Constantinou, M., Hadjiloizou, P., Kika, K. and Constantinou, P., Pesticide residues analysis in honey using ethyl acetate extraction method: validation and pilot survey in real samples. Int. J. Environ. Anal. Chem., 2015, 95(10), 894–910.

  • Bhardwaj, T. and Sharma, J. P., Impact of pesticides application in agricultural industry: an Indian scenario. Int. J. Agric. Food Sci. Technol., 2013, 4(8), 817–822.

  • Aktar, M. W., Sengupta, D. and Chowdhury, A., Impact of pest icides use in agriculture: their benefits and hazards. Interdis. Toxicol., 2009, 2(1), 1–12.

  • Fernandez-Muino, M. A., Sancho, M. T., Muniategui, S., Huidobro, J. F. and Simal-Lozano, J., Nonacaricide pesticide residues in honey: analytical methods and levels found. J. Food Protect., 1995, 58(11), 1271–1274.

  • Kujawski, M. W., Barganska, Z., Marciniak, K., Miedzianowska, E., Kujawski, J. K., Slebioda, M. and Namiesnik, J., Determining pesticide contamination in honey by LC-ESI-MS/MS- Comparison of pesticide recoveries of two liquid-liquid extraction based approaches. LWT – Food Sci. Technol., 2014, 56, 517–523.

  • Mullin, C. A., Frazier, M., Frazier, J. L., Ashcraft, S., Simonds, R., vanEngelsdorp, D. and Pettis, J. S., High levels of miticides and agrochemicals in North American apiaries: implications for honey bee health. PLoS ONE, 2010, 5, e9754(3).

  • Naccari, C., Macaluso, A., Giangrosso, G., Naccari, F. and Ferrantelli, V., Risk assessment of heavy metals and pesticides in honey from Sicily (Italy). J. Food Res., 2014, 3, 107.

  • Porrini, C. et al., Honey bees and bee products as monitors of the environmental contamination. Apiacta, 2003, 38, 63-70.

  • Aliferis, K. A., Tarantilis, P. A., Harizanis, P. C. and Alissandrakis, E., Botanical discrimination and classification of honey samples applying gas chromatography/mass spectrometry fingerprinting of headspace volatile compounds. Food Chem., 2010, 121(3), 856–862.

  • Brouwer, A. et al., Characterization of potential endocrine related health effects at low dose levels of exposure to PCBs. Environ. Health Perspect., 1999, 107, 639.

  • Crisp, T. M. et al., Environmental endocrine disruption: an effects assessment and analysis. Environ. Health Perspect., 1999, 106, 11.

  • Hurley, P. M., Hill, R. N. and Whiting, R. J., Mode of carcinogenic action of pesticides inducing thyroid follicular cell tumours in rodents. Environ. Health Perspect., 1998, 106, 437.

  • Fernandez, M., Pico, Y. and Manes, J., Analytical methods for pesticide residue determination in bee products. J. Food. Prot., 2002, 65(9), 1502–1511.

  • Blasco, C., Fernández, M., Pena, A., Lino, C., Silveira, M. I., Font, G. and Picó, Y., Assessment of pesticide residues in honey samples from Portugal and Spain. J. Agric. Food Chem., 2003, 51(27), 8132–8138.

  • Debayle, D., Dessalces, G. and Grenier-Loustalot, M. F., Multiresidue analysis of traces of pesticides and antibiotics in honey by HPLC-MS-MS. Anal. Bioanal. Chem., 2008, 391(3), 1011–1020.

  • De Pinho, G. P., Neves, A. A., de Queiroz, M. E. L. R. and Silverio, F. O., Optimization of the liquid–liquid extraction method and low temperature purification (LLE–LTP) for pesticide residue analysis in honey samples by gas chromatography. Food Control, 2010, 21(10), 1307–1311.

  • Rissato, S. R., Galhiane, M. S., de Almeida, M. V., Gerenutti, M. and Apon, B. M., Multiresidue determination of pesticides in honey samples by gas chromatography–mass spectrometry and application in environmental contamination. Food Chem., 2007, 101(4), 1719–1726.

  • Anastassiades, M., Lehotay, S. J., Tajnbaher, D. and Schenck, F. J., Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce. J. AOAC Int., 2003, 86(2), 412–431.

  • Barakat, A., Badawy, H. M. A., Salama, E., Attallah, E. and Maatook, G., Simple and rapid method of analysis for determination of pesticide residues in honey using dispersive solid phase extraction and GC determination. J. Food, Agric. Environment, 2007, 5(2), 97–100.

  • Barganska, Z., Slebioda, M. and Namiesnik, J., Determination of pesticide residues in honeybees using modified QUEChERS sample work-up and liquid chromatography-tandem mass spectrometry. Molecules, 2014, 19(3), 2911–2924.

  • Malhat, F. M., Haggag, M. N., Loutfy, N. M., Osman, M. A. M. and Ahmed, M. T., Residues of organochlorine and synthetic pyrethroid pesticides in honey, an indicator of ambient environment, a pilot study. Chemosphere, 2015, 120, 457–461.

  • Irungu, J., Raina, S. and Torto, B., Determination of pesticide residues in honey: a preliminary study from two of Africa’s largest honey producers. Int. J. Food Contam., 2016, 3(1), 14.

  • AOAC (Association of Official Analytical Chemist). AOAC official method 2007.01: pesticide residues in foods by acetonitri le extraction and partitioning with magnesium sulfate. AOAC Int., Gaithersburg, USA, 2007.

  • EC (European Commission). Document number SANTE/11945/2015. Guidance document on analytical quality control and method validation procedures for pesticides residues analysis in food and feed, 2016.

  • ICH (International Conference on Harmonisation). Harmonised Tripartite Guideline Validation of Analytical Procedures: text and methodology Q2 (R1), 2005.

  • Eissa, F., El-Sawi, S. and Zidan, N. E., Determining pesticide residues in honey and their potential risk to consumers. Pol. J. Environ. Stud., 2014, 23(5), 1573–1580.

  • Lalhmangaihi, R. and Laha, R. C., Beekeeping: a nature eco-friendly business. In Science and Technology for Shaping Future of Mizoram – Proceedings of the Mizoram Science Congress (ed. Lalchhandama, K.), Allied Publishers, New Delhi, 2017, pp. 35–39.

  • FAO/WHO. Pesticide residues in food. In Report of the joint meeting of the FAO panel of experts on pesticide residues in food and the environment and the WHO core assessment group on pesticide residues. FAO plant production and protection paper, 2016, p. 229; http://www.fao.org/fileadmin/templates/agphome/documents/Pests_Pesticides/JMPR/Report2016/JMPR_2016_Report_full.pdf. (accessed on 13 September 2017).

  • EC (European Commission). Regulation (EC) No 396/2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending council directive 91/414/EEC. Official J. Eur. Commun., 2005, pp. 1–16;. http://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX%3A32005 R0396 (accessed on 15 March 2016).

  • EIC (Export Inspection Council), India. Honey residue monitoring plan (RMP) for export to EU Year 2017-18. EIC/RMP/HN/201718; Rev-0. https://www.eicindia.gov.in/Services/Pre-Compliance/ PDF/RMP%202017-18%20Honey.pdf (accessed on 25 November 2017).

  • Kumari, B., Madan, V. K. and Kathpal, T. S., Status of insecticide contamination of soil and water in Haryana, India. Environ. Monit. Assess., 2008, 136(1), 239–244.

  • Lari, S. Z., Khan, N. A., Gandhi, K. N., Meshram, T. S. and Thacker, N. P., Comparison of pesticide residues in surface water and ground water of agriculture intensive areas. J. Environ. Health Sci. Eng., 2014, 12(1), 11.

  • Ananda G. S. R. and Somashekar, R. K., Evaluation of pesticide residues in farmgate samples of vegetables in Karnataka, India. Bull. Environ. Contam. Toxicol., 2012, 89(3), 626–632.

  • Harinathareddy, A., Prasad, N. B. L. and Devi, L. K., Pesticide residues in vegetable and fruit samples from Andhra Pradesh, India. J. Biol. Chem. Res., 2014, 31, 1005–1015.

  • Bedi, J. S., Gill, J. P. S., Aulakh, R. S. and Kaur, P., Occurrence and spatial distribution of pesticide residues in butter and ghee (clarified butter fat) in Punjab (India). Environ. Monit. Assess., 2016, 188(2), 100.

  • Ramesh, B. K. and Selvanayagam, M., Pesticides pollution in water, sediment and fishes of Kolavai lake in Chengalpet, Tamil Nadu, India. Int. J. Chem. Concepts, 2015, 1, 9–14.

  • Directorate of plant protection, quarantine and storage, Government of India. State-wise estimated demand of chemical pesticides during 2011–12 to 2015–16 and projected demand for 2016–17, 2017; http://ppqs.gov.in/divisions/pesticides-monitoring-documentation. (accessed on 14 March 2017).

  • Panseri, S., Catalano, A., Giorgi, A., Arioli, F., Procopio, A., Britti, D. and Chiesa, L. M., Occurrence of pesticide residues in Italian honey from different areas in relation to its potential contamination sources. Food Control, 2014, 38, 150–156.


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  • Multiresidue Determination of Pesticides in Market Honey from Northern India Using QuEChERS Approach and Assessment of Potential Risks to Consumers

Abstract Views: 409  |  PDF Views: 118

Authors

Atul Kumar
CSK HP Agricultural University, Palampur - 176 062, India
Jatinder Paul Singh Gill
Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, India
Jasbir Singh Bedi
Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, India

Abstract


Honey has multiple beneficial properties but polluted environments have led to its contamination. Contaminated honey not only serves as a sentinel of environmental pollution, but can also pose potential risks to consumers’ health. In the present study, QuEChERS (quick, easy, cheap, effective, rugged and safe) method along with gas chromatography coupled to selective detectors (ECD/FTD/MS) was used for determining 24 pesticide residues and/or their metabolites in 150 honey samples collected from markets in Northern India. The method was optimized and validated according to the European Commission’s guidelines. Residues of pesticides were detected in 12% of samples, of which a majority contained organophosphate residues. Assessment of human health risks suggests that contaminated honey at current levels has minimal contribution to toxicological risks. However, precautionary measures should always be taken considering the customary honey feeding in infants and cumulative effect of these chemicals in the foreseeable future. This study highlights the importance of continuous monitoring of pesticide residues, and consumer awareness towards certified products to safeguard public health.

Keywords


Honey, Northern India, Pesticides, Risk Assessment, QuEChERS.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi2%2F283-291