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Optimization of Factors Affecting Decolourization of Sulphonated Azo Dye Red HE7b in vitro by bacillus Sp. Azo1


Affiliations
1 Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi - 110 012, India
2 ICAR-Indian Institute of Soil Science, Nabibagh, Berasia Road, Bhopal - 462 038, India
 

A zo dyes are widely used organic molecules that are generally recalcitrant to biodegradation, thus causing environmental pollution. In this study, the effect of various growth conditions like temperature, pH, dye concentration, aeration and presence/absence of co-substrate on decolourization of azo dye Red HE7B by Bacillus sp. Azo1 has been determined. Decolourization of the dye was achieved under static condition. Optimum decolourization of Red HE7B was observed at 30°C and 50 mg/l dye concentration, where more than 90% decolourization was achieved within three days of inoculation. Bacillus sp. Azo1 could decolourize the dye through a range of pH values from 6 to 9. Presence of co-substrate limited the decolourization with glucose being the most potential inhibitor. Identification of factors influencing decolourization will help in rapid removal of these pollutants from contaminated habitat.

Keywords

Azo Dye, Bacillus Sp., Co-Substrate, Decolourization.
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  • Rawat, D., Mishra, V. and Sharma, R. S., Detoxification of azo dyes in the context of environmental processes. Chemosphere, 2016, 155, 591–605.

  • Solís, M., Solis, A., Perez, H. I., Manjarrez, N. and Flores, M., Microbial decolouration of azo dyes: a review. Process Biochem., 2012, 47, 1723–1748.

  • Talarposhti, M. A., Donnelly, T. and Anderson, G. K., Colour removal from a simulated dye wastewater using a two-phase anaerobic packed bed reactor. Water Res., 2001, 35, 425–432.

  • Chen, H., Recent advances in azo dye degrading enzyme research. Curr. Protein Pept. Sci., 2006, 7, 101–111.

  • Bruschweiler, B. J. and Merlot, C., Azo dyes in clothing textiles can be cleaved into a series of mutagenic aromatic amines which are not regulated yet. Regul. Toxicol. Pharmacol., 2017, 88, 214–226.

  • Elbanna, K., Sarhan, O. M., Khider, M., Elmogy, M., Abulreesh, H. H. and Shaaban, M. R., Microbiological, histological, and biochemical evidence for the adverse effects of food azo dyes on rats. J. Food Drug Anal., 2017, 25, 667–680.

  • Thakur, J. K., Paul, S., Dureja, P., Annapurna, K., Padaria, J. C. and Gopal, M., Degradation of sulphonated azo dye Red HE7B by Bacillus sp. and elucidation of degradative pathways. Curr. Microbiol., 2014, 69, 183–191.

  • Chen, K. C., Wua, J. Y., Huang, C. C., Liang, Y. M. and Hwang, S. C. J., Decolorization of azo dye using PVA-immobilized microorganisms. J. Biotechnol., 2003, 101, 241–252.

  • Kalyani, D. C., Telke, A. A., Dhanve, R. S. and Jadhav, J. P., Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. J. Hazard. Mater., 2009, 163, 735–742.

  • Telke, A., Kalyani, D., Jadhav, J. and Govindwar, S., Kinetics and mechanism of Reactive Red 141 degradation by a bacterial isolate Rhizobium radiobacter MTCC 8161. Acta Chim. Slov., 2008, 55, 320–329.

  • Mabrouk, M. E. M. and Yusef, H. H., Decolorization of Fast Red by Bacillus subtilis HM. J. Appl. Sci. Res., 2008, 4, 262–269.

  • Pandey, A., Singh, P. and Iyengar, L., Bacterial decolourization and degradation of azo dye. Int. Biodeterior. Biodegrad., 2007, 59, 73–84.

  • Zimmermann, T., Kulla, H. G. and Leisinger, T., Properties of purified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas luteola. Bioresour. Technol., 1982, 49, 47–51.

  • Banat, I. M., Nigam, P., Singh, D. and Marchant, R., Microbial decolorization of textile-dye-containing effuents: a review. Bioresour. Technol., 1996, 58, 217–227.

  • Khan, Z., Jain, K., Soni, A. and Madamwar, D., Microaerophilic degradation of sulphonated azo dye – Reactive Red 195 by bacterial consortium AR1 through co-metabolism. Int. Biodeterior. Biodegrad., 2014, 94, 167–175.

  • Wuhrmann, K., Mechsner, K. and Kappeler, T., Investigation in the rate determining factors in the microbial reduction of azo dyes. Eur. J. Appl. Microbiol. Biotechnol., 1980, 9, 325–338.

  • Ogawa, T., Shibata, M., Yatome, C. and Idaka, E., Growth inhibition of Bacillus subtilis by basic dyes. Bull. Environ. Contam. Toxicol., 1988, 40, 545–552.

  • Parshetti, G. K., Telke, A. A., Kalyani, D. C. and Govindwar, S. P., Decolorization and detoxification of sulfonated azo dye Methyl Orange by Kocuria rosea MTCC 1532. J. Hazard. Mater., 2010, 176, 503–509.

  • Gottlieb, A., Shaw, C., Smith, A., Wheatley, A. and Forsythe, S., The toxicity of textile reactive azo dyes after hydrolysis and decolourization. J. Biotechnol., 2003, 101, 49–56.

  • Gallert, C., Bauer, S. and Winter, J., Effect of ammonia on the anaerobic degradation of protein by a mesophilic and thermophilic biowaste population. Appl. Microbiol. Biotechnol., 1998, 50, 495–501.

  • Berman, N. and Rettger, L. F., The influence of carbohydrate on the nitrogen metabolism of bacteria. J. Bacteriol., 1917, 3, 389–402.

  • Van der Zee, F. P., Lettinga, G. and Field, J. A., Azo dye decolourisation by anaerobic granular sludge. Chemosphere, 2001, 44, 1169–1176.

  • Cerboneschi, M., Corsi, M., Bianchini, R., Bonanni, M. and Tegli, S., Decolorization of acid and basic dyes: understanding the metabolic degradation and cell-induced adsorption/precipitation by Escherichia coli. Appl. Microbiol. Biotechnol., 2015, 99, 8235–8245.

  • Carliell, C. M., Barelay, S. J., Naidoo, N., Buckley, C. A., Mulholland, D. A. and Senior, E., Microbial decolourization of a reactive azo dye under anaerobic conditions. Water S.A., 1995, 21, 61–69.

  • Silva, S. Q., Silva, D. C., Lanna, M. C. S., Baeta, B. E. L. and Aquino, S. F., Microbial dynamics during azo dye degradation in a UASB reactor supplied with yeast extract. Braz. J. Microbiol., 2014, 45, 1153–1160.

  • Chang, J. S., Chen, B. Y. and Lin, Y. S., Stimulation of bacterial decolorization of an azo dye by extracellular metabolites from Escherichia coli strain NO3. Bioresour. Technol., 2004, 91, 243–248.


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  • Optimization of Factors Affecting Decolourization of Sulphonated Azo Dye Red HE7b in vitro by bacillus Sp. Azo1

Abstract Views: 319  |  PDF Views: 112

Authors

Jyoti Kumar Thakur
Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi - 110 012, India
Sangeeta Paul
Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi - 110 012, India
Nishant K. Sinha
ICAR-Indian Institute of Soil Science, Nabibagh, Berasia Road, Bhopal - 462 038, India
Maheshwar Singh Rathi
Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi - 110 012, India

Abstract


A zo dyes are widely used organic molecules that are generally recalcitrant to biodegradation, thus causing environmental pollution. In this study, the effect of various growth conditions like temperature, pH, dye concentration, aeration and presence/absence of co-substrate on decolourization of azo dye Red HE7B by Bacillus sp. Azo1 has been determined. Decolourization of the dye was achieved under static condition. Optimum decolourization of Red HE7B was observed at 30°C and 50 mg/l dye concentration, where more than 90% decolourization was achieved within three days of inoculation. Bacillus sp. Azo1 could decolourize the dye through a range of pH values from 6 to 9. Presence of co-substrate limited the decolourization with glucose being the most potential inhibitor. Identification of factors influencing decolourization will help in rapid removal of these pollutants from contaminated habitat.

Keywords


Azo Dye, Bacillus Sp., Co-Substrate, Decolourization.

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DOI: https://doi.org/10.18520/cs%2Fv115%2Fi3%2F505-509