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A Comparative Study on Biodegradation of Chlorpyrifos by Wild E. coli and Pseudomonas fluorescens Bacterial Isolates Inhabiting Different Ecosystems of Kashmir Valley


Affiliations
1 Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
2 Department of Biotechnology, School of Life Sciences, Central University of Kashmir, Srinagar 190 004, India
3 RCRQ Laboratory, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
4 Department of Biotechnology, Amity University, Sector-125, Noida 201 313, India
 

Among 1081 naturally occurring wild isolates exam-ined for E. coli and Pseudomonas fluorescens, EC1 (E. coli) from Dal Lake (Srinagar district) and PF1 (P. fluorescens) from soil samples of Ganderbal district showed maximum tolerance (11 mg/ml) towards chlorpyrifos. The high performance liquid chroma-tography (HPLC) based chlorpyrifos (CP) degrada-tion analysis demonstrated that each isolate degraded chlorpyrifos much more efficiently than the reference strain E. coli MTCC-533 used in the current study. The present study suggests that EC1 and PF1 bacteri-al isolates inhabiting different ecosystems, degrade chlorpyrifos efficiently via genetic determinants and OPP enzymatic system and provide strong basis for development of bioremediation strategies in the area.

Keywords

Bioremediation, Chlorpyrifos, E. coli, HPLC, Pseudomonas fluorescens, Resistance.
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  • A Comparative Study on Biodegradation of Chlorpyrifos by Wild E. coli and Pseudomonas fluorescens Bacterial Isolates Inhabiting Different Ecosystems of Kashmir Valley

Abstract Views: 320  |  PDF Views: 123

Authors

Imtiyaz Murtaza
Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
Bushra
Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
Sageera Showkat
Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
Shah Ubaid-Ullah
Department of Biotechnology, School of Life Sciences, Central University of Kashmir, Srinagar 190 004, India
Omi Laila
Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
Sumyra Majid
Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
Neyiaz A. Dar
Biochemistry and Molecular Biotechnology Laboratory, Biochemistry Section, Division of Basic Sciences, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
Mukhtar Ahmad
RCRQ Laboratory, SKUAST-K, Shalimar Campus, Srinagar 190 025, India
Girish Sharma
Department of Biotechnology, Amity University, Sector-125, Noida 201 313, India

Abstract


Among 1081 naturally occurring wild isolates exam-ined for E. coli and Pseudomonas fluorescens, EC1 (E. coli) from Dal Lake (Srinagar district) and PF1 (P. fluorescens) from soil samples of Ganderbal district showed maximum tolerance (11 mg/ml) towards chlorpyrifos. The high performance liquid chroma-tography (HPLC) based chlorpyrifos (CP) degrada-tion analysis demonstrated that each isolate degraded chlorpyrifos much more efficiently than the reference strain E. coli MTCC-533 used in the current study. The present study suggests that EC1 and PF1 bacteri-al isolates inhabiting different ecosystems, degrade chlorpyrifos efficiently via genetic determinants and OPP enzymatic system and provide strong basis for development of bioremediation strategies in the area.

Keywords


Bioremediation, Chlorpyrifos, E. coli, HPLC, Pseudomonas fluorescens, Resistance.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi4%2F753-758