Current Science

Open Access Open Access  Restricted Access Subscription Access

Occurrence of Fusarium Species in Asymptomatic Barley Plants Grown in Field Conditions Under Differential Phosphate Regimes


Affiliations
1 CONICET-Universidad de Buenos Aires, Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), Buenos Aires, Argentina
2 CONICET-BIOLAB (CICBA-INBIOTEC-CONICET), Facultad de Agronomía (UNCPBA), Microbiología, Rep. de Italia No. 780, Azul B7300BZD, Buenos Aires, Argentina
 

Occurrence of genus Fusarium as endophytes in ischolar_mains of healthy barley plants cultivated in fields under two levels of phosphorous (P) was examined. F. avenaceum, F. graminearum, F. konzum, F. oxysporum, F. sacchari, F. scirpi, F. solani were isolated from asymptomatic surface sterilized ischolar_main tissues. F. konzum and F. solani were only present in the ischolar_mains of fertilized plants, whereas F. sacchari was present in unfertilized plants. F. scirpi was isolated in highest frequency and relative density, and was higher under P deficiency suggesting that P availability would determinate the occurrence of this species, suggesting the possibility of their association with stressed plants. This is the first report on the occurrence of Fusarium spp. as endophytes in healthy barley plants. However, it remains to be determined whether Fusarium spp. isolated are nonpathogenic forms and beneficial to barley plants or latent pathogens that could produce disease symptoms in other conditions.

Keywords

Barley (HGordeum Vulgare L.), Endophytic Fungi, Fusarium Species, Latent Pathogens, Nutritional Regime.
User
Notifications
Font Size

  • Leslie, J. F. and Summerell, B. A., The Fusarium Laboratory Manual, Blackwell Publishing, Ames, Iowa, USA, 1st edn, 2006.

  • Griffin, M. R., Biocontrol and bioremediation: two areas of endophytic research which hold great promise. In Advances in Endophytic Research (eds Verma, V. C. and Gange, A. C.), Springer India, 2014, pp. 257–282.

  • Stone, J. K., Bacon, C. W. and White Jr, J. F., An overview of endophytic microbes: endophytism defined. In Microbial Endophytes (eds Bacon, C. W. and White Jr, J. F.), Marcel Dekker, New York, 2000, pp. 3–29.

  • Kuldau, G. A. and Yates, I. E., Evidence for Fusarium endophytes in cultivated and wild plants. In Microbial Endophytes (eds Bacon, C. W. and White Jr, J. F.), Marcel Dekker, New York, 2000, pp. 85–117.

  • Petrini, O., Fungal endophytes of tree leaves. In Microbial Ecology of Leaves (eds Andrews, J. H. and Hirano, S. S.), SpringerVerlag, New York, 1991, pp. 179–197.

  • Schulz, B. and Boyle, C., The endophytic continuum. Mycol. Res., 2005, 109, 661–686.

  • Maciá-Vicente, J. G., Jansson, H.-B., Abdullah, S. K., Descals, E., Salinas, J. and Lopez-Llorca, L. V., Fungal ischolar_main endophytes from natural vegetation in Mediterranean environments with special reference to Fusarium spp. FEMS Microbiol. Ecol., 2008, 64, 90–105.

  • Rodrigues Costa Pinto, L. S., Azevedo, J. L., Pereira, J. O., Carneiro Vieira, M. L. and Labate, C. A., Symptomless infection of banana and maize by endophytic fungi impairs photosynthetic efficiency. New Phytol., 2000, 147, 609–615.

  • Diene, O. and Narisawa, K., The use of symbiotic fungal associations with crops in sustainable agriculture. J. Dev. Sust. Agric., 2009, 4, 50–56.

  • DeBernardi, L., La cebada. Gacetilla informativa del sector agrícola. Newsletter Nro. 81. Dirección de Mercados Agrícolas. Ministerio de Agroindustria, Argentina, 2015; www.minagri.gob.ar/dimeagro

  • Cattaneo, M., Los mercados de cebada cervecera en Argentina y en el mundo. In Cebada cervecera (eds Miralles, D. J., BenechArnold, R. L. and Abeledo, L. G.), Editorial Facultad de Agronomía, Buenos Aires, 2011, pp. 275–284.

  • Briggs, D. E., Boulton, C. A., Brookes, P. A. and Stevens, R., Chemical and physical properties of beer. In Brewing Science and Practice, Elsevier Science and Technology, Woodhead Publishing Limited and CRC Press, LLC, 2004, pp. 662–715.

  • McGonigle, T. P., Miller, M. H., Evans, D. G., Fairchild, G. L. and Swan, J. A., A new method which gives an objective measure of colonization of ischolar_mains by vesicular–arbuscular mycorrhizal fungi. New Phytol., 1990, 115, 495–501.

  • Fisher, N. L., Burguess, L. W., Toussoun, T. A. and Nelson, P. E., Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology, 1982, 72, 151–153.

  • Nelson, P. E., Toussoun, T. A. and Marasas, W. F. O., Fusarium Species: An Illustrated Manual for Identification, The Pennsylvania State University Press, University Park, PA, USA, 1983.

  • Stenglein, S. A. and Balatti, P. A., Genetic diversity of Phaeoisariopsis griseola in Argentina as revealed by pathogenic and molecular markers. Physiol. Mol. Plant P., 2006, 68(4–6), 158–167.

  • Mishra, P. K., Fox, R. T. V. and Culhan, A., Development of a PCR-based assay for rapid and reliable identification of pathogenic Fusaria. FEMS Microbiol. Lett., 2003, 218, 329–332.

  • Jurado, M., Vasquez, C., Patiño, B. and Gonzalez-Jaen, M. T., PCR detection assays for the trichotecene producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides. Syst. Appl. Microbiol., 2005, 28, 562–568.

  • Marasas, W. F. O., Burgess, L. W., Anelich, R. Y., Lamprecht, S. C. and Van Schalkwyk, D. J., Survey of Fusarium species associated with plant debris in South African soils. S. Afr. J. Bot., 1988, 54, 63–71.

  • Di Rienzo, J. A., Casanoves, F., Balzarini, M. G., Gonzalez, L., Tablada, M. and Robledo, C. W. InfoStat version 2015. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina; http://www.infostat.com.ar

  • Bacon, C. W. and White Jr, J. F. (eds), Physiological adaptations in the evolution of endophytism in the clavicipitaceae. In Microbial Endophytes, Marcel Dekker, New York, 2000, pp. 341–388.

  • Aly, A. H., Debbab, A. and Proksch, P., Fungal endophytes: unique plant inhabitants with great promises. Appl. Microbiol. Biotehnol., 2011, 90, 1829–1845.

  • Hartley, S. E. and Gange, A. C., Impacts of plant symbiotic fungi on insect herbivores: mutualism in a multitrophic context. Annu. Rev. Entomol., 2009, 54, 323–342.

  • Saikkonen, K., Saari, S. S. and Helander, M., Defensive mutualism between plants and endophytic fungi? Fungal Divers., 2010, 41, 101–113.

  • Photita, W., Lumyong, S., Lumyoung, P., McKenzie, E. H. C. and Hyde, K. D., Are some endophytes of Musa acuminata latent pathogens? Fungal Divers., 2004, 16, 131–140.

  • Sanchez Marquez, S., Bills, G. F., Herrero, N. and Zabalgogeazcoa, I., Non-systemic fungal endophytes of grasses. Fungal Ecol., 2012, 5, 289–297.

  • Currie, A. F., Wearn, J., Hodgson, S., Wendt, H., Broughton, S. and Jin, L., Foliar Fungal endophytes in herbaceous plants: a marriage of convenience? In Advances in Endophytic Research (eds Verma, V. C. and Gange, A. C.), New Delhi, 2014, pp. 61–81.

  • Le, H. T. T., Padgham J. L. and Sikora R. A., Biological control of the rice ischolar_main-knot nematode Meloidogyne graminicola on rice, using endophytic and rhizosphere fungi. Int. J. Pest Manage., 2009, 55(1), 31–36.

  • Demers, J. E., Gugino, B. K. and Jiménez-Gasco, M. M., Highly diverse endophytic and soil Fusarium oxysporum populations associated with field-grown tomato plants. Appl Environ Microbiol., 2014, 81(1), 81–90.

  • Nalini, M. S., Sunayana, N. and Prakash, H. S., Endophytic fungal diversity in medicinal plants of Western Ghats, India. Int. J. Biodivers., 2014, 2014, 1–9; https://doi.org/10.1155/2014/494213.

  • Gomes Fernandes, E., Liparini Pereira, O., Cânedo da Silva, C., Braga Pereira Bento, C. and Vieira de Queiroz, M., Diversity of endophytic fungi in Glycine max. Microbiol. Res., 2015, 181, 84–92.

  • Nelson, P. E., Horst, R. K. and Woltz, S. S., Fusarium diseases of ornamental plants. In Fusarium: Diseases, Biology and Taxonomy. (eds Nelson, P. E., Toussoun, T. A. and Cook, R. J.), The Pennsylvania State University Press, University Park, London, 1981, pp. 121–128.

  • Olivain, C., Trouvelot, S., Binet, M. N., Cordier, C., Pugin, A. and Alabouvette, C., Colonization of flax ischolar_mains and early physiological responses of flax cells inoculated with pathogenic and nonpathogenic strains of Fusarium oxysporum. Appl. Environ. Microbiol., 2003, 69, 5453–5462.

  • Athman, S. Y., Dubois, T., Coyne, D., Gold, C. S., Labuschagne, N. and Viljoen, A., Effect of Fusarium oxysporum on host preference of Radapholus similis to tissue culture bananas plants. J. Nematol., 2006, 38(4), 455–460.

  • Ting, A. S. Y., Meon, S., Kadir, J., Radu, S. and Singh, G., Endophytic microorganisms as potential growth-promoters of banana. Biocontrol., 2008, 53, 541–553.

  • Ting, A. S. Y., Meon, S., Kadir, J., Radu, S. and Singh, G., Induced host resistance by non-pathogenic Fusarium endophyte as a potential defence mechanism in Fusarium wilt management of banana. Pest Technol., 2009, 3, 67–72.

  • Cabello, M. and Arambarri, A., Diversity in soil fungi from undisturbed and disturbed Celtis tala and Scutia buxifolia forests in the eastern Buenos Aires province (Argentina). Microbiol. Res., 2002, 157, 115–125.

  • Díaz Arias, M. M., Munkvold, G. P., Ellis, M. L. and Leandro, L. F. S., Distribution and frequency of Fusarium species associated with soybean ischolar_mains in Iowa. Plant Dis., 2013, 97(12), 1557–1562.

  • Silvestro, L. B., Stenglein, S. A., Forjan, H., Dinolfo, M. I., Arambarri, A. M., Manso, L. and Moreno, M. V., Occurrence and distribution of soil Fusarium species under wheat crop in zero tillage, Span. J. Agric. Res., 2013, 11(1), 72–79.

  • Edel-Hermann, V., Gautheron, N., Mounier, A. and Steinberg, C., Fusarium diversity in soil using a specific molecular approach and a cultural approach. J. Microbiol. Meth., 2015, 111, 64–71.

  • Castañares, E., Albuquerque, D. R., Dinolfo, M. I., Pinto, V. F., Patriarca, A. and Stenglein, S. A., Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina. Int. J. Food. Microbiol., 2014, 179, 57–63.

  • Lacicowa, B. and Kiecana, I., Fusariosis of spring barley cultivated in Lublin (Polonia) region. Mycotoxin Res., 1991, 2, 128–135.

  • Piacentini, K. C., Savi, G. D., Pereira, M. E. and Scussel, V. M., Fungi and the natural occurrence of deoxynivalenol and fumonisins in malting barley (Hordeum vulgare L.). Food Chem., 2015, 187, 204–209.

  • Zeller, K. A., Summerell, B. A., Bullock, S. and Leslie, J. F., Gibberella konza (Fusarium konzum) sp. nov. from prairie grasses, a new species in the Gibberella fujikuroi species complex. Mycologia, 2003, 95(5), 943–954.

  • Nur Ain Izzati, M. Z. and Wan Hasmida, W. I., Isolation of microfungi from Malay traditional vegetables and secondary metabolites produced by Fusarium species. Sains Malays., 2011, 40(5), 437–444.

  • Brum, M. C., Araújo, W. L., Maki, C. S. and Azevedo, J. L., Endophytic fungi from Vitis labrusca L. (‘Niagara Rosada’) and its potential for the biological control of Fusarium oxysporum. Genet. Mol. Res., 2012, 11(4), 4187–4197.

  • Petrovic, T., Burgess, L. W., Cowie, I., Warren, R. A. and Harvey, P. R., Diversity and fertility of Fusarium sacchari from wild rice (Oryza australiensis) in Northern Australia, and pathogenicity tests with wild rice, rice, sorghum and maize. Eur. J. Plant Pathol., 2013, 136(4), 773–788.

  • Fisher, P. J. and Petrini, O., Fungal saprobes and pathogens as endophytes of rice (Oryza sativa L.). New Phytol., 1992, 120, 137–143.

  • Bezerra, J. D. P. et al., Fungal endophytes from cactus Cereus jamacaru in Brazilian tropical dry forest: a first study. Symbiosis, 2013, 60(2), 53–63.

  • Latiffah, Z. and Ning, C. H., Endophytic Fusarium spp. from ischolar_mains of lawn grass (Axonopus compressus). Trop. Life Sci. Res., 2013, 24(2), 85–90.

  • Criado, M. V., Roberts, I. N., Veliz, C. G., Echeverria, M., Gutierrez Boem, F. H. and Caputo, C., Phloem transport of assimilates in relation ti flowering time and senescence in barley grown with different availabilities of nitrogen and phosphorus. Arch. Agron. Soil. Sci., 2018, 64(4), 492–504.

  • Kogel, K.-H., Franken, P. and Hückelhoven, R., Endophyte or parasite – what decides? Curr. Opin. Plant Biol., 2006, 9, 358–363.

  • Shukla, A., Dehariya, K., Vyas, D. and Jha, A., Interactions between arbuscular mycorrhizae and Fusarium oxysporum f. sp. ciceris: effects on fungal development, seedling growth and wilt disease suppression in Cicer arietinum L. Arch. Phytopathol. Pfl., 2014, 48, 240–252.

  • Dwivedi, S. K., Sangeeta, K. and Gopal, R., Role of mycorrhizae as biofertilizer and bioprotectant. Int. J. Pharm. Bio Sci., 2015, 6(2), B1014–B1026.

  • Ren, L., Zhang N., Wu, P., Huo, H., Xu, G. and Wu, G., Arbuscular mycorrhizal colonization alleviates Fusarium wilt in watermelon and modulates the composition of ischolar_main exudates. Plant Growth Regul., 2015, 77(1), 77–85.

  • Bourdages, J. V., Marchand, S., Rioux, S. and Belzile, F. J., Diversity and prevalence of Fusarium species from Quebec barley fields. Can. J. Plant Pathol., 2006, 28(3), 419–425.

  • Fernandez, M. R., Holzgang, G. and Turkington, T. K., Common ischolar_main rot of barley in Saskatchewan and north-central Alberta. Can. J. Plant Pathol., 2009, 31(1), 96–102.

  • Astolfi., P., dos Santos, J., Schneider, L., Gomes, L. B., Silva, C. N., Tessmann, D. J. and Del Ponte, E. M., Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in Southern Brazil. Int. J. Food Microbiol., 2011, 148(3), 197–201.

  • Beccari, G., Caproni, L., Tini, F., Uhlig, S. and Covarelli, L., Presence of Fusarium species and other toxigenic fungi in malting barley and multi-mycotoxin analysis by liquid chromatography– high-resolution mass spectrometry. J. Agric. Food Chem., 2016, 64(21), 4390–4399.

  • Hsuan, H. M., Salleh, B. and Zakaria, L., Molecular identification of Fusarium species in Gibberella fujikuroi species complex from rice, sugarcane and maize from Peninsular Malaysia. Int. J. Mol. Sci., 2011, 12(10), 6722–6732.

  • Poongothai, M., Viswanathan, R., Malathi, P. and Ramesh Sundar, A., Sugarcane wilt: pathogen recovery from different tissues and variation in cultural characters. Sugar Tech., 2014, 16(1), 50–66.


Abstract Views: 450

PDF Views: 121




  • Occurrence of Fusarium Species in Asymptomatic Barley Plants Grown in Field Conditions Under Differential Phosphate Regimes

Abstract Views: 450  |  PDF Views: 121

Authors

Mariela Echeverria
CONICET-Universidad de Buenos Aires, Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), Buenos Aires, Argentina
Maria Virginia Moreno
CONICET-BIOLAB (CICBA-INBIOTEC-CONICET), Facultad de Agronomía (UNCPBA), Microbiología, Rep. de Italia No. 780, Azul B7300BZD, Buenos Aires, Argentina
Carla Caputo
CONICET-Universidad de Buenos Aires, Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), Buenos Aires, Argentina

Abstract


Occurrence of genus Fusarium as endophytes in ischolar_mains of healthy barley plants cultivated in fields under two levels of phosphorous (P) was examined. F. avenaceum, F. graminearum, F. konzum, F. oxysporum, F. sacchari, F. scirpi, F. solani were isolated from asymptomatic surface sterilized ischolar_main tissues. F. konzum and F. solani were only present in the ischolar_mains of fertilized plants, whereas F. sacchari was present in unfertilized plants. F. scirpi was isolated in highest frequency and relative density, and was higher under P deficiency suggesting that P availability would determinate the occurrence of this species, suggesting the possibility of their association with stressed plants. This is the first report on the occurrence of Fusarium spp. as endophytes in healthy barley plants. However, it remains to be determined whether Fusarium spp. isolated are nonpathogenic forms and beneficial to barley plants or latent pathogens that could produce disease symptoms in other conditions.

Keywords


Barley (HGordeum Vulgare L.), Endophytic Fungi, Fusarium Species, Latent Pathogens, Nutritional Regime.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi5%2F939-944