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Fish Short-Term Reproductive Assay for Evaluating the Estrogenic Property of a Commonly used Antioxidant, Butylated Hydroxyanisole


Affiliations
1 School of Ocean Sciences and Technology, Kerala University of Fisheries and Ocean Sciences, Kochi 682 506, India
2 Department of Zoology, Mar Athanasius College, Kothamangalam 686 666, India
3 Department of Zoology, University College, Thiruvananthapuram 695 034, India
 

Fish short-term reproductive assay (FSTRA) is a tier one assay for screening the endocrine disrupting property of a compound. This study analysed the end points like variations in Gonado-Somatic index (GSI), sperm quality and count, serum hormone levels (FSH, LH, estrogen and testosterone) to study the endocrine disrupting properties of butylated hydroxyanisole (BHA). A fresh water teleost, Anabas testudineus was used as animal model in this study. LC50 value of BHA was 250 mg/kg body weight in fish. Effects of four different doses of BHA were studied - 2, 5, 50 and 75 mg/kg body weight. BHA is found toxic at high doses of 250 mg/kg body weight and above. All the four doses of BHA caused significant impact on GSI, sperm count and quality. Serum hormone assay by ELISA showed significant variations in treated groups compared to control group.

Keywords

Anabas testudineus, Anisole, Endocrine Disruption.
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  • Fish Short-Term Reproductive Assay for Evaluating the Estrogenic Property of a Commonly used Antioxidant, Butylated Hydroxyanisole

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Authors

George Paul
School of Ocean Sciences and Technology, Kerala University of Fisheries and Ocean Sciences, Kochi 682 506, India
R. N. Binitha
Department of Zoology, Mar Athanasius College, Kothamangalam 686 666, India
Francis Sunny
Department of Zoology, University College, Thiruvananthapuram 695 034, India

Abstract


Fish short-term reproductive assay (FSTRA) is a tier one assay for screening the endocrine disrupting property of a compound. This study analysed the end points like variations in Gonado-Somatic index (GSI), sperm quality and count, serum hormone levels (FSH, LH, estrogen and testosterone) to study the endocrine disrupting properties of butylated hydroxyanisole (BHA). A fresh water teleost, Anabas testudineus was used as animal model in this study. LC50 value of BHA was 250 mg/kg body weight in fish. Effects of four different doses of BHA were studied - 2, 5, 50 and 75 mg/kg body weight. BHA is found toxic at high doses of 250 mg/kg body weight and above. All the four doses of BHA caused significant impact on GSI, sperm count and quality. Serum hormone assay by ELISA showed significant variations in treated groups compared to control group.

Keywords


Anabas testudineus, Anisole, Endocrine Disruption.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi8%2F1584-1587