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Biological Control of Potato Tuber Soft Rot using N-Acyl-L-Homoserine Lactone-Degrading Endophytic Bacteria


Affiliations
1 Agricultural Genetics Institute, Pham Van Dong, Tu Liem, Hanoi, Viet Nam
2 Soils and Fertilizers Research Institute, Dong Ngac, Tu Liem, Hanoi, Viet Nam
3 Ministry of Agriculture and Rural Development, Ngoc Ha, Ba Dinh, Hanoi, Viet Nam
4 Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
 

Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103–104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.

Keywords

AHL, Pectobacterium carotovorum, Potato Tuber Soft Rot, Quorum Sensing, Quorum Quenching.
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  • Biological Control of Potato Tuber Soft Rot using N-Acyl-L-Homoserine Lactone-Degrading Endophytic Bacteria

Abstract Views: 315  |  PDF Views: 110

Authors

Nguyen Thanh Ha
Agricultural Genetics Institute, Pham Van Dong, Tu Liem, Hanoi, Viet Nam
Tran Quang Minh
Soils and Fertilizers Research Institute, Dong Ngac, Tu Liem, Hanoi, Viet Nam
Pham Xuan Hoi
Agricultural Genetics Institute, Pham Van Dong, Tu Liem, Hanoi, Viet Nam
Nguyen Thi Thanh Thuy
Ministry of Agriculture and Rural Development, Ngoc Ha, Ba Dinh, Hanoi, Viet Nam
Naruto Furuya
Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
Hoang Hoa Long
Agricultural Genetics Institute, Pham Van Dong, Tu Liem, Hanoi, Viet Nam

Abstract


Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 103–104 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.

Keywords


AHL, Pectobacterium carotovorum, Potato Tuber Soft Rot, Quorum Sensing, Quorum Quenching.

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DOI: https://doi.org/10.18520/cs%2Fv115%2Fi10%2F1921-1927