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Comparative Expression Analysis of Defence-Related Genes in Bacillus-Treated Glycine Max upon Challenge Inoculation with Selective Fungal Phytopathogens


Affiliations
1 Mandsaur University, Mandsaur 458 001, India
2 Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India
 

Activation of defence-related genes by the application of beneficial bacteria leads to prior protection against pathogens through induced systemic resistance. The present study was carried out to examine the qRT– PCR-based relative quantification of differently expressed defence-related genes in soybean (Glycine max L. Merrill) plants primed with Bacillus sp. strain SJ-5 against the fungal pathogen Rhizoctonia solani and Fusarium oxysporum. In this context, molecular characterization of plant growth promoting and biocontrol genes of SJ-5 was done by PCR followed by homology analysis. In the GC-MS analysis of SJ-5 volatile organic compounds, potent antifungal compound bis(2-ethyl-hexyl) phthalate and antioxidant compound butylated hydroxy toluene were reported with the highest peak area 47.96% and 21.82% respectively, along with other antifungal compounds in small proportion. Qualitative expression of different defence-related genes like lipoxygenase, phenylalanine ammonia-lyase 2, peroxidase, polyphenol oxidase, endo-1,3-beta-glucanase, catalase, defensin-like protein, vegetative storage protein and chitinase class I was found elicited in the plants primed with SJ-5 against the fungal pathogens. In the qPCR analysis, the highest upregulation was observed in the transcript profile of ppojh2 in the treatments T5 and T6 with 4.12- and 4.06-fold increase respectively.

Keywords

Defence-Related Genes, Induced Systemic Resistance, Plant Growth Promoting Bacteria, Volatile Organic Compounds.
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  • Hashem, E. A., Abdalla, H. E., Hussein, Y. A. and Abd-Elnabi, M. A., In vitro selection of soybean callus resistant to Fusarium oxysporum metabolites. Res. J. Agric. Biol. Sci., 2009, 5, 588–596.

  • Jain, S., Varma, A., Tuteja, N. and Choudhary, D. K., Plant growth promoting microbial-mediated induced systemic resistance in plants: induction, mechanism and expression. In MicrobialMediated Induced Systemic Resistance in Plants (eds Choudhary, D. K. and Varma, A.), Springer, Singapore, 2016, pp. 213–226.

  • Pieterse, C. M. J., Leon-Reyes, A., Van der Ent, S. and Van Wees, S. C. M., Networking by small-molecule hormones in plant immunity. Nature Chem. Biol., 2009, 5, 308–316.

  • Yang, Y. X., Ahammed, G. J., Wu, C., Fan, S. Y. and Zhou, Y. H., Crosstalk among jasmonate, salicylate and ethylene signaling pathways in plant disease and immune responses. Curr. Protein Pept. Sci., 2015, 16, 450–461.

  • McConn, M., Creelman, R. A., Bell, E., Mullet, J. E. and Browse, J., Jasmonate is essential for insect defense in Arabidopsis. Proc. Natl. Acad. Sci. USA, 1997, 94, 5473–5477.

  • Mauch, F., Mauch-Mani, B. and Boller, T. Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinases and b-1, 3-glucanase. Plant Physiol., 1988, 88, 936–942.

  • Ryu, C. M., Hu, C. H., Reddy, M. S. and Kloepper, J. W., Different signaling pathways of induced resistance by rhizobacteria in Arabidopsis thaliana against two pathovars of Pseudomonas syringae. New Phytol., 2003, 160, 413–420.

  • Choudhary, D. K., Sharma, K. P. and Gaur, R. K., Biotechnological perspectives of microbes in agro-ecosystems. Biotechnol. Lett., 2011, 33, 1905–1910.

  • Bollet, C., Gevaudan, M. J., de Lamballerie, X., Zandotti, C. and de Micco, P., A simple method for the isolation of chromosomal DNA from Gram positive or acid fast bacteria. Nucleic Acids Res., 1991, 19, 1955.

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S., MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol., 2013, 30, 2725–2729.

  • Jain, S., Vaishnav, A., Kumari, S., Varma, A., Tuteja, N. and Choudhary, D. K., Chitinolytic Bacillus-mediated induction of jasmonic acid and defense-related proteins in soybean (Glycine max L. Merrill) plant against Rhizoctonia solani and Fusarium oxysporum. J. Plant Growth. Regul., 2017, 36, 200–214.

  • Deubel, A., Gransee, A. and Merbach, W., Transformation of organic rhizodeposits by rhizoplane bacteria and its influence on the availability of tertiary calcium phosphate. J. Plant Nutr. Soil. Sci., 2000, 163, 387–392.

  • de Werra, P., Péchy-Tarr, M., Keel, C. and Maurhofer, M., Role of gluconic acid production in the regulation of biocontrol traits of Pseudomonas fluorescens CHA0. Appl. Environ. Microbiol., 2009, 75, 4162–4174.

  • Spaepen, S., Vanderleyden, J. and Remans, R., Indole-3-acetic acid in microbial and microorganism–plant signaling. FEMS Microbiol. Rev., 2007, 31, 425–448.

  • Sun, Y., Cheng, Z. and Glick, B. R., The presence of a 1-aminocyclopropane1-carboxylate (ACC) deaminase deletion mutation alters the physiology of the endophytic plant growth-promoting bacterium Burkholderia phytofirmans PsJN. FEMS Microbiol. Lett., 2009, 296, 131–136.

  • Xu, T., Zhu, T. and Li, S., β-1, 3-1, 4-glucanase gene from Bacillus velezensis ZJ20 exerts antifungal effect on plant pathogenic fungi. World J. Microbiol. Biotechnol., 2016, 32, 1–9.

  • Yang, C. Y., Ho, Y. C., Pang, J. C., Huang, S. S. and Tschen, J. S., Cloning and expression of an antifungal chitinase gene of a novel Bacillus subtilis isolate from Taiwan potato field. Bioresour. Technol., 2009, 100, 1454–1458.

  • Beligni, M. V., Fath, A., Bethke, P. C., Lamattina, L. and Jones, R. L., Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers. Plant Physiol., 2002, 129, 1642–1650.

  • Kavitha, A., Prabhakar, P., Vijayalakshmi, M. and Venkateswarlu, Y., Production of bioactive metabolites by Nocardia levis MK-VL_113. Lett. Appl. Microbiol., 2009, 49, 484–490.

  • Al-Bari, M. A., Rahman, M. S., Islam, M. A., Flores, M. E. and Bhuiyan, M. S., Purification and characterization of a β-(1,4)-endoxylanase of Streptomyces bangladeshiensis sp. Res. J. Cell. Mol. Biol., 2007, 1, 31–36.

  • Hoang, V. L., Li, Y. and Kim, S. K., Cathepsin B inhibitory activities of phthalates isolated from a marine Pseudomonas strain. Bioorg. Med. Chem. Lett., 2008, 18, 2083–2088.

  • Namikoshi, M., Fujiwara, T., Nishikawa, T. and Ukai, K., Natural abundance 14C content of dibutyl phthalate (DBP) from three marine algae. Mar. Drugs, 2006, 4, 290–297.

  • Amade, P., Mallea, M. and Bouaicha, N., Isolation, structural identification and biological activity of two metabolites produced by Penicillium olsonii Bainier and Sartory. J. Antibiot., 1994, 47, 201–207.

  • Ahsan, T., Chen, J., Zhao, X., Irfan, M. and Wu, Y., Extraction and identification of bioactive compounds (Eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf. AMB Exp., 2017, 7, 54.

  • Kether, F. B., Mahjoub, M. A., Mahjoub, S. A., Salah, K. B., Helal, A. N. and Mighri, Z., Chemical composition, in vitro antifungal and antioxidant activities of essential oil from Cotula coronopifolia L. growing in Tunisia. Afr. J. Microbiol. Res., 2012, 6, 4388–4395.

  • Pohl, C. H., Kock, J. L. and Thibane, V. S., Antifungal free fatty acids: a review. In Science against Microbial Pathogens: Current Research and Technological Advances (ed. Mendez-Vilas, A.), FORMATEX Research Center, Spain, 2011, pp. 61–71.

  • Barrera-Necha, L. L., Garduno-Pizana, C. and Garcia-Barrera, L. J., In vitro antifungal activity of essential oils and their compounds on mycelial growth of Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen. Plant Pathol. J., 2009, 8, 17–21.

  • Desmond, O. J., Edgar, C. I., Manners, J. M., Maclean, D. J., Schenk, P. M. and Kazan, K., Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum. Physiol. Mol. Plant Pathol., 2006, 67, 171–179.

  • Ebadzad, G. and Cravador, A., Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection. Springerplus, 2014, 3, 1–13.

  • Dos Santos, C. V., Letousey, P., Delavault, P. and Thalouarn, P., Defense gene expression analysis of Arabidopsis thaliana parasitized by Orobanche ramosa. Phytopathology, 2003, 93, 451–457.


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  • Comparative Expression Analysis of Defence-Related Genes in Bacillus-Treated Glycine Max upon Challenge Inoculation with Selective Fungal Phytopathogens

Abstract Views: 356  |  PDF Views: 110

Authors

Shekhar Jain
Mandsaur University, Mandsaur 458 001, India
Anukool Vaishnav
Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India
Ajit Varma
Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India
Devendra Kumar Choudhary
Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India

Abstract


Activation of defence-related genes by the application of beneficial bacteria leads to prior protection against pathogens through induced systemic resistance. The present study was carried out to examine the qRT– PCR-based relative quantification of differently expressed defence-related genes in soybean (Glycine max L. Merrill) plants primed with Bacillus sp. strain SJ-5 against the fungal pathogen Rhizoctonia solani and Fusarium oxysporum. In this context, molecular characterization of plant growth promoting and biocontrol genes of SJ-5 was done by PCR followed by homology analysis. In the GC-MS analysis of SJ-5 volatile organic compounds, potent antifungal compound bis(2-ethyl-hexyl) phthalate and antioxidant compound butylated hydroxy toluene were reported with the highest peak area 47.96% and 21.82% respectively, along with other antifungal compounds in small proportion. Qualitative expression of different defence-related genes like lipoxygenase, phenylalanine ammonia-lyase 2, peroxidase, polyphenol oxidase, endo-1,3-beta-glucanase, catalase, defensin-like protein, vegetative storage protein and chitinase class I was found elicited in the plants primed with SJ-5 against the fungal pathogens. In the qPCR analysis, the highest upregulation was observed in the transcript profile of ppojh2 in the treatments T5 and T6 with 4.12- and 4.06-fold increase respectively.

Keywords


Defence-Related Genes, Induced Systemic Resistance, Plant Growth Promoting Bacteria, Volatile Organic Compounds.

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DOI: https://doi.org/10.18520/cs%2Fv115%2Fi10%2F1950-1956