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Artificial Seed for Short-Term Storage:Using Nodal Buds in Aquilaria malaccensis Lam


Affiliations
1 Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
 

Artificial seed technology is known to be the best alternative for short-term storage of plant germplasm in recalcitrant species. Aquilaria malaccensis, being a recalcitrant seeded tree, possesses seasonal and highly desiccation-sensitive seeds which lose viability with slight decrease in their moisture content, hence storage becomes a difficult task. The present study deals with short-term storage of A. malaccensis nodal buds through artificial seed technology. Nodal buds encapsulated with 2.5% sodium alginate and 100 mM calcium chloride, and polymerized for 40 min were found suitable for artificial seed preparation. Maximum regeneration rate of 83.3% and 75.0% was observed from encapsulated nodal buds stored at 4°C and 23 ± 2°C respectively, for 10 days. Storage was possible for a period of 60 days at 4°C and 50 days at 23 ± 2°C with average regeneration rate of 8.3% and 16.7% respectively.

Keywords

Artificial Seeds, Encapsulation, Recalcitrant, Storage, Temperature.
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  • Artificial Seed for Short-Term Storage:Using Nodal Buds in Aquilaria malaccensis Lam

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Authors

Seram Devika Devi
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
Bateimon Kharsahnoh
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
Suman Kumaria
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
Meera C. Das
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India

Abstract


Artificial seed technology is known to be the best alternative for short-term storage of plant germplasm in recalcitrant species. Aquilaria malaccensis, being a recalcitrant seeded tree, possesses seasonal and highly desiccation-sensitive seeds which lose viability with slight decrease in their moisture content, hence storage becomes a difficult task. The present study deals with short-term storage of A. malaccensis nodal buds through artificial seed technology. Nodal buds encapsulated with 2.5% sodium alginate and 100 mM calcium chloride, and polymerized for 40 min were found suitable for artificial seed preparation. Maximum regeneration rate of 83.3% and 75.0% was observed from encapsulated nodal buds stored at 4°C and 23 ± 2°C respectively, for 10 days. Storage was possible for a period of 60 days at 4°C and 50 days at 23 ± 2°C with average regeneration rate of 8.3% and 16.7% respectively.

Keywords


Artificial Seeds, Encapsulation, Recalcitrant, Storage, Temperature.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi11%2F2103-2109