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Synthesis and Characterization of Nano-selenium and its Antibacterial Response on Some Important Human Pathogens


Affiliations
1 Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram - 624 302, India
 

Synthesis of nano-selenium was achieved from sodium selenite by a simple precipitation method using the reducing power of ascorbic acid. The high-speed centrifuge was used to separate selenium nanoparticles from aqueous solution. The selenium nanoparticles were characterized by UV–Vis spectroscopy, X-ray diffraction, Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy, energy dispersive X-ray analysis and transmission electron microscope. Presence of various functional groups responsible for the production and stability of the nanoparticles was confirmed by FT-IR. Some of the important human pathogens like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used for examining the antibacterial response of selinium nanoparticles. Results of this study demonstrate that synthesized selenium nanoparticles exhibit a spherical shape with average diameter range between 15 and 18 nm. They can be used as an antibacterial agent and also in medicinal applications for the treatment of humans with certain bacterial diseases.

Keywords

Antibacterial Activity, Characterization, Human Pathogens, Synthesis, Selenium Nanoparticles.
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  • Mongillo, F. J., Nanotechnology, Greenwood Press, Westport, Connecticut, USA, 2007, p. 101.

  • Maynard, A. D., Baron, P. A., Foley, M., Shvedova, A. A., Kisin, E. R. and Castranova, V., Exposure to carbon nanotube material: aerosol release during the handling of unrefined single-walled carbon nanotube material. J. Toxicol. Environ. Health, Part A, 2004, 67, 87-108.

  • Mohanraj, V. J. and Chen, Y., Nanoparticles - a review. Trop. J. Pharm. Res., 2006, 5(1), 561-573.

  • Wang, Y. and Herron, N., Nanometer-sized semiconductor clusters: materials synthesis, quantum size effects, and photophysical properties. J. Phys. Chem., 1991, 95, 525-532.

  • Romero-Perez, A., Garcia-Garcia, E. and Zavaleta-Manceraetal, A., Designing and evaluation of sodium selenite nanoparticles in vitro to improve selenium absorption in ruminants. Vet. Res. Commun., 2010, 34, 71-79.

  • Nath, S., Ghosh, S. K., Panigahi, S., Thundat, T. and Pal, T., Synthesis of selenium nanoparticle and its photocatalytic application for decolorization of methylene blue under UV irradiation. Langmuir, 2004, 20, 7880.

  • Cremonini, E. et al., Biogenic selenium nanoparticles: characterization, antimicrobial activity and effects on human dendritic cells and fibroblasts. Microb. Biotechnol., 2016, 9(6), 758-771.

  • Nguyen, T. H. D., Vardhanabhuti, B., Lin, M. and Mustapha, A., Antibacterial properties of selenium nanoparticles and their toxicity to Caco-2 cells. Food Control, 2017, 77, 17-24.

  • Choi, O., Deng, K. K., Kim, N. J., Ross Jr, L., Surampalli, R. Y. and Hu, Z., The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth. Water Res., 2008, 42(12), 3066-3074.

  • Yip, J., Liu, L., Wong, K. H., Leung, P. H., Yuen, C. W. M. and Cheung, M. C., Investigation of antifungal and antibacterial effects of fabric padded with highly stable selenium nanoparticles. J. Appl. Polym. Sci., 2014, 131(17), 40728 (1-8).

  • Kheradmand, E., Rafii, F., Yazdi, M. H., Sepahi, A. A., Shahverdi, A. R. and Oveisi, M. R., The antimicrobial effects of selenium nanoparticle-enriched probiotics and their fermented broth against Candida albicans. DARU, 2014, 22(1), 48.

  • Shakibaie, M., Foischolar_mainanfar, H., Golkari, Y., MohammadiKhorsand, T. and Shakibaie, M. R., Anti-biofilm activity of biogenic selenium nanoparticles and selenium dioxide against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Proteus mirabilis. J. Trace Elem. Med. Biol., 2015, 29, 235-241.

  • Malhotra, S., Jha, N. and Desai, K., A superficial synthesis of selenium nanospheres using wet chemical approach. Int. J. Nanotechnol. Appl., 2014, 3(4), 7-14.

  • Li, Q., Chen, T. and Yang, F., Facile and controllable one-step fabrication of selenium nanoparticles assisted by L-cysteine. Mater. Lett., 2010, 64, 614-617.

  • Khurana, J. M. and Vij, K., Nickel nanoparticles as semiheterogeneous catalyst for one-pot, three-component synthesis of 2-amino4H-pyrans and pyran annulated heterocyclic moieties. Synth.

  • Commun., 2013, 43(17), 2294-2304.

  • Fesharaki, P. J., Nazari, P., Shakibaie, M., Rezaie, S., Banoee, M., Abdollahi, M. and Shahverdi, A. R., Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process. Braz. J. Microbiol., 2010, 41, 461- 466.

  • Zhang, W., Chen, Z., Liu, H., Zhang, L., Gao, P. and Li, D., Biosynthesis and structural characteristics of selenium nanoparticles by Pseudomonas alcaliphila. Colloids Surf., B, 2011, 88, 196-201.

  • Harikrishnan, H., Abdullah, N. A., Ponmurugan, K. and Shyam Kumar, R., Microbial synthesis of selenium nanocomposite using Saccharomyces cerevisiae and its antimicrobial activity against pathogens causing nosocomial infection. Chalcogenide Lett., 2012, 9, 509-515.

  • Dorofeey, G. A., Streletskii, A. N., Povstugar, I. V., Protasov, A. V. and Elsukov, E. P., Determination of nanoparticle sizes by X-ray diffraction. Colloid J., 2012, 74, 675-685.

  • Khiralla, G. M. and El-Deeb, B. A., Antimicrobial and antibiofilm effects of selenium nanoparticles on some food borne pathogens. LWT - Food Sci. Technol., 2015, 63, 1001-1007.

  • Prasad, K. S., Patel, H., Patel, T., Patel, K. and Selvaraj, K., Biosynthesis of Se nanoparticles and its effect on UV-induced DNA damage. Colloids Surf. B, 2013, 103, 261-266.

  • Mallikarjuna, K., Narasimha, G., Dillip, G. R., Praveen, B. and Shreedhar, B., Green synthesis of silver nanoparticles using Ocimum leaf extract and their characterization. Dig. J. Nanomater. Biostruct., 2011, 6, 181-186.

  • Pal, D., Mishra, P., Sachan, N. and Ghosh, A. K., Biological activities and medicinal properties of Cajanus cajan (L) Millsp. J. Adv. Pharm. Technol. Res., 2011, 2(4), 207-214.

  • Hariharan, Al-Dhabi, N. A., Karuppiah, P. and Rajaram, S. K., Microbial synthesis of selenium nanocomposite using Saccharomyces cerevisiae and its antimicrobial activity against pathogens causing nosocomial infection. Chalcogenide Lett., 2012, 12, 509- 515.

  • Razi, K., Sarraf Maamoury, M. R. and Banihashemi, S., Preparation of nano selenium particles by water solution phase method from industrial dust. Int. J. Nano Dimens., 2011, 1, 261-267.

  • Keerthika, V., Ramesh, R. and Rajan, M. R., Toxicity assessment of iron oxide nanoparticles in Labeo rohita. Int. National J. Fish. Aquat. Stud., 2017, 5(4), 1-6.

  • Hu, C. H., Li, Y. I., Xiong, L., Zhnag, H. M., Song, J. and Xia, M. S., Comparative effects of nano elemental selenium and sodium selenite on selenium retention in broiler chickens. Anim. Feed Sci. Technol., 2012, 177, 204-210.

  • Chen, T., Wonga, Y.-S., Zhengb, W., Baib, Y. and Huangb, L., Selenium nanoparticles fabricated in Undaria pinnatifida polysaccharide solutions include mitochondria-mediated apotosis in A375 human melanoma cells. Colloids Surf. B, 2008, 67, 26-31.

  • Hamouda, T. et al., A novel surfactant nanoemulsion with broadspectrum sporicidal activity against Bacillus species. Infect. Dis., 1999, 180, 1939.

  • Klabunde, K. J. et al., Nanocrystals as stoichiometric reagents with unique surface chemistry. Phys. Chem., 1996, 12, 142.

  • Stoimenov, P. K., Klinger, R. L., Marchin, G. L. and Klabunde, K. J., Metal oxide nanoparticles as bactericidal agents. Langmuir, 2002, 18, 6679.

  • Zhang, L., Jiang, Y., Ding, Y., Malcolm, P. and David, Y., Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids). J. Nanopart. Res., 2007, 9, 479.

  • Chudobova, D., Cihalova, K., Dostalova, S., Ruttkay-Nedecky, B., Rodrigo, M. A. M. and Tmejova, K., Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection. FEMS Microbiol. Lett., 2014, 351(2), 195-201.


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  • Synthesis and Characterization of Nano-selenium and its Antibacterial Response on Some Important Human Pathogens

Abstract Views: 346  |  PDF Views: 97

Authors

Ananth Angamuthu
Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram - 624 302, India
Keerthika Venkidusamy
Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram - 624 302, India
Ruby Rajan Muthuswami
Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram - 624 302, India

Abstract


Synthesis of nano-selenium was achieved from sodium selenite by a simple precipitation method using the reducing power of ascorbic acid. The high-speed centrifuge was used to separate selenium nanoparticles from aqueous solution. The selenium nanoparticles were characterized by UV–Vis spectroscopy, X-ray diffraction, Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy, energy dispersive X-ray analysis and transmission electron microscope. Presence of various functional groups responsible for the production and stability of the nanoparticles was confirmed by FT-IR. Some of the important human pathogens like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used for examining the antibacterial response of selinium nanoparticles. Results of this study demonstrate that synthesized selenium nanoparticles exhibit a spherical shape with average diameter range between 15 and 18 nm. They can be used as an antibacterial agent and also in medicinal applications for the treatment of humans with certain bacterial diseases.

Keywords


Antibacterial Activity, Characterization, Human Pathogens, Synthesis, Selenium Nanoparticles.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi2%2F285-290